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Preparation, characterization and in vivo evaluation of alginate-coated chitosan and trimethylchitosan nanoparticles loaded with PR8 influenza virus for nasal immunization.
Asian J Pharm Sci. 2019 Mar; 14(2):216-221.AJ

Abstract

For efficient mucosal vaccine delivery, nanoparticulate antigens are better taken by microfold cells in the nasal associated lymphoid tissue and also dendritic cells. Nanoparticles based on polymers such as chitosan (CHT) and its water soluble derivative, trimethylchitosan (TMC), could be successfully used as carrier/adjuvant for this purpose. Sodium alginate, a negatively charged biopolymer, could modify the immunostimulatory properties of CHT and TMC NPs and increase their stability. Sodium alginate (ALG)-coated chitosan (CHT) and trimethylchitosan (TMC) nanoparticles (NPs) loaded with inactivated PR8 influenza virus were successfully prepared by direct coating of the virus with CHT or TMC polymers to evaluate their immunoadjuvant potential after nasal immunization. After nasal immunizations in BALB/c mice, PR8-CHT formulation elicited higher IgG2a and IgG1 antibody titers compared with PR8-TMC. ALG coating of this formulation (PR8-CHT-ALG) significantly decreased the antibody titers and a less immune response was induced than PR8-TMC-ALG formulation. PR8-TMC-ALG formulation showed significantly higher IgG2a/IgG1 ratio, as criteria for Th1-type immune response, compared with PR8-CHT-ALG and PR8 virus alone. Altogether, the PR8-TMC-ALG formulation could be considered as an efficient intranasal antigen delivery system for nasal vaccines.

Authors+Show Affiliations

Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32104453

Citation

Mosafer, Jafar, et al. "Preparation, Characterization and in Vivo Evaluation of Alginate-coated Chitosan and Trimethylchitosan Nanoparticles Loaded With PR8 Influenza Virus for Nasal Immunization." Asian Journal of Pharmaceutical Sciences, vol. 14, no. 2, 2019, pp. 216-221.
Mosafer J, Sabbaghi AH, Badiee A, et al. Preparation, characterization and in vivo evaluation of alginate-coated chitosan and trimethylchitosan nanoparticles loaded with PR8 influenza virus for nasal immunization. Asian journal of pharmaceutical sciences. 2019;14(2):216-221.
Mosafer, J., Sabbaghi, A. H., Badiee, A., Dehghan, S., & Tafaghodi, M. (2019). Preparation, characterization and in vivo evaluation of alginate-coated chitosan and trimethylchitosan nanoparticles loaded with PR8 influenza virus for nasal immunization. Asian Journal of Pharmaceutical Sciences, 14(2), 216-221. https://doi.org/10.1016/j.ajps.2018.04.005
Mosafer J, et al. Preparation, Characterization and in Vivo Evaluation of Alginate-coated Chitosan and Trimethylchitosan Nanoparticles Loaded With PR8 Influenza Virus for Nasal Immunization. Asian journal of pharmaceutical sciences. 2019;14(2):216-221. PubMed PMID: 32104453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation, characterization and in vivo evaluation of alginate-coated chitosan and trimethylchitosan nanoparticles loaded with PR8 influenza virus for nasal immunization. AU - Mosafer,Jafar, AU - Sabbaghi,Amir-Hossein, AU - Badiee,Ali, AU - Dehghan,Solmaz, AU - Tafaghodi,Mohsen, Y1 - 2018/05/10/ PY - 2017/11/23/received PY - 2018/03/14/revised PY - 2018/04/06/accepted PY - 2020/2/28/entrez PY - 2020/2/28/pubmed PY - 2020/2/28/medline KW - Alginate KW - Chitosan KW - Nasal immunization KW - PR8 influenza virus KW - Trimethyl chitosan SP - 216 EP - 221 JF - Asian journal of pharmaceutical sciences VL - 14 IS - 2 N2 - For efficient mucosal vaccine delivery, nanoparticulate antigens are better taken by microfold cells in the nasal associated lymphoid tissue and also dendritic cells. Nanoparticles based on polymers such as chitosan (CHT) and its water soluble derivative, trimethylchitosan (TMC), could be successfully used as carrier/adjuvant for this purpose. Sodium alginate, a negatively charged biopolymer, could modify the immunostimulatory properties of CHT and TMC NPs and increase their stability. Sodium alginate (ALG)-coated chitosan (CHT) and trimethylchitosan (TMC) nanoparticles (NPs) loaded with inactivated PR8 influenza virus were successfully prepared by direct coating of the virus with CHT or TMC polymers to evaluate their immunoadjuvant potential after nasal immunization. After nasal immunizations in BALB/c mice, PR8-CHT formulation elicited higher IgG2a and IgG1 antibody titers compared with PR8-TMC. ALG coating of this formulation (PR8-CHT-ALG) significantly decreased the antibody titers and a less immune response was induced than PR8-TMC-ALG formulation. PR8-TMC-ALG formulation showed significantly higher IgG2a/IgG1 ratio, as criteria for Th1-type immune response, compared with PR8-CHT-ALG and PR8 virus alone. Altogether, the PR8-TMC-ALG formulation could be considered as an efficient intranasal antigen delivery system for nasal vaccines. SN - 2221-285X UR - https://www.unboundmedicine.com/medline/citation/32104453/Preparation_characterization_and_in_vivo_evaluation_of_alginate_coated_chitosan_and_trimethylchitosan_nanoparticles_loaded_with_PR8_influenza_virus_for_nasal_immunization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1818-0876(17)30926-1 DB - PRIME DP - Unbound Medicine ER -
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