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Lactobacillus delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets after a Lipopolysaccharide Challenge.
Oxid Med Cell Longev. 2020; 2020:6028606.OM

Abstract

This study was conducted to evaluate the effect of dietary supplementation with Lactobacillus delbrueckii (LAB) on intestinal morphology, barrier function, immune response, and antioxidant capacity in weaned piglets challenged with lipopolysaccharide (LPS). A total of 36 two-line crossbred (Landrace × large Yorkshire) weaned piglets (28 days old) were divided into three groups: (1) nonchallenged control (CON); (2) LPS-challenged control (LPS); and (3) LAB+LPS treatment (0.2% LAB+LPS). Compared to the LPS piglets, the LAB+LPS piglets improved intestinal morphology, indicated by greater (P < 0.05) villus height in the duodenum and ileum; villus height : crypt depth ratio in the duodenum, jejunum, and ileum, as well as decreased (P < 0.05) crypt depth in the jejunum and ileum; and better intestinal barrier function, indicated by upregulated (P < 0.05) mRNA expression of tight junction proteins in the intestinal mucosa. Moreover, compared to the LPS piglets, LAB significantly decreased (P < 0.05) concentrations of TNF-α and IL-1β in the small intestine and increased (P < 0.05) IL-10 levels in the jejunum and ileum. Additionally, LAB increased (P < 0.05) T-AOC activities of the colon, GSH concentrations of the jejunum, and mRNA expression of CAT and Cu/Zn-SOD, while reduced (P < 0.05) MDA concentrations in the jejunum and ileum in LPS-changed piglets. Collectively, our results indicate that supplementation of LAB improved intestinal integrity and immune response and alleviated intestinal oxidative damage in LPS-challenged piglets.

Authors+Show Affiliations

College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China. Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha Hunan 410128, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32104535

Citation

Chen, Fengming, et al. "Lactobacillus Delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets After a Lipopolysaccharide Challenge." Oxidative Medicine and Cellular Longevity, vol. 2020, 2020, p. 6028606.
Chen F, Wang H, Chen J, et al. Lactobacillus delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets after a Lipopolysaccharide Challenge. Oxid Med Cell Longev. 2020;2020:6028606.
Chen, F., Wang, H., Chen, J., Liu, Y., Wen, W., Li, Y., & Huang, X. (2020). Lactobacillus delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets after a Lipopolysaccharide Challenge. Oxidative Medicine and Cellular Longevity, 2020, 6028606. https://doi.org/10.1155/2020/6028606
Chen F, et al. Lactobacillus Delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets After a Lipopolysaccharide Challenge. Oxid Med Cell Longev. 2020;2020:6028606. PubMed PMID: 32104535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lactobacillus delbrueckii Ameliorates Intestinal Integrity and Antioxidant Ability in Weaned Piglets after a Lipopolysaccharide Challenge. AU - Chen,Fengming, AU - Wang,Houjun, AU - Chen,Jiayi, AU - Liu,Yang, AU - Wen,Wei, AU - Li,Yinghui, AU - Huang,Xingguo, Y1 - 2020/02/10/ PY - 2019/12/02/received PY - 2020/01/24/accepted PY - 2020/2/28/entrez PY - 2020/2/28/pubmed PY - 2020/9/22/medline SP - 6028606 EP - 6028606 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2020 N2 - This study was conducted to evaluate the effect of dietary supplementation with Lactobacillus delbrueckii (LAB) on intestinal morphology, barrier function, immune response, and antioxidant capacity in weaned piglets challenged with lipopolysaccharide (LPS). A total of 36 two-line crossbred (Landrace × large Yorkshire) weaned piglets (28 days old) were divided into three groups: (1) nonchallenged control (CON); (2) LPS-challenged control (LPS); and (3) LAB+LPS treatment (0.2% LAB+LPS). Compared to the LPS piglets, the LAB+LPS piglets improved intestinal morphology, indicated by greater (P < 0.05) villus height in the duodenum and ileum; villus height : crypt depth ratio in the duodenum, jejunum, and ileum, as well as decreased (P < 0.05) crypt depth in the jejunum and ileum; and better intestinal barrier function, indicated by upregulated (P < 0.05) mRNA expression of tight junction proteins in the intestinal mucosa. Moreover, compared to the LPS piglets, LAB significantly decreased (P < 0.05) concentrations of TNF-α and IL-1β in the small intestine and increased (P < 0.05) IL-10 levels in the jejunum and ileum. Additionally, LAB increased (P < 0.05) T-AOC activities of the colon, GSH concentrations of the jejunum, and mRNA expression of CAT and Cu/Zn-SOD, while reduced (P < 0.05) MDA concentrations in the jejunum and ileum in LPS-changed piglets. Collectively, our results indicate that supplementation of LAB improved intestinal integrity and immune response and alleviated intestinal oxidative damage in LPS-challenged piglets. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/32104535/Lactobacillus_delbrueckii_Ameliorates_Intestinal_Integrity_and_Antioxidant_Ability_in_Weaned_Piglets_after_a_Lipopolysaccharide_Challenge_ L2 - https://doi.org/10.1155/2020/6028606 DB - PRIME DP - Unbound Medicine ER -