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Molecular and pharmacological characterization of a β-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps.
Insect Biochem Mol Biol. 2020 05; 120:103337.IB

Abstract

As the counterparts of noradrenaline and adrenaline in vertebrates, octopamine (OA) regulates multiple physiological and behavioral processes in invertebrate. OA mediates its effects via binding to specific octopamine receptors (OARs). Functional and pharmacological characterization of OARs have been reported in several insects. However, little work was documented in hemipteran insects. We cloned a β-adrenergic-like OAR (NcOA2B2) from Nephotettix cincticeps. NcOA2B2 shares high similarity with members of the OA2B2 receptor class. Transcript level of NcOA2B2 varied in various tissues and was highly expressed in the leg. After heterologous expression in CHO-K1 cells, NcOA2B2 was dose-dependently activated by OA (EC50 = 2.56 nM) and tyramine (TA) (EC50 = 149 nM). Besides putative octopaminergic agonists, dopaminergic agonists and amitraz and DPMF potently activated NcOA2B2 in a dose-dependent manner. Receptor activity was blocked by potential antagonists and was most efficiently antagonized by asenapine. Phentolamine showed both antagonist and agonist effects on NcOA2B2. Our results offer the important information about molecular and pharmacological characterization of an OAR from N. cincticeps that will provide the basis for forthcoming studies on its roles in physiological processes and behaviors, and facilitate the design of novel insecticides for pest control.

Authors+Show Affiliations

State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China. Electronic address: xugang@yzu.edu.cn.State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address: changxuefei2015@163.com.State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address: gu_guixiang@163.com.State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address: 573024808@qq.com.State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address: 1115143760@qq.com.State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address: huangj@zju.edu.cn.State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address: chu@zju.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32109588

Citation

Xu, Gang, et al. "Molecular and Pharmacological Characterization of a Β-adrenergic-like Octopamine Receptor From the Green Rice Leafhopper Nephotettix Cincticeps." Insect Biochemistry and Molecular Biology, vol. 120, 2020, p. 103337.
Xu G, Chang XF, Gu GX, et al. Molecular and pharmacological characterization of a β-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps. Insect Biochem Mol Biol. 2020;120:103337.
Xu, G., Chang, X. F., Gu, G. X., Jia, W. X., Guo, L., Huang, J., & Ye, G. Y. (2020). Molecular and pharmacological characterization of a β-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps. Insect Biochemistry and Molecular Biology, 120, 103337. https://doi.org/10.1016/j.ibmb.2020.103337
Xu G, et al. Molecular and Pharmacological Characterization of a Β-adrenergic-like Octopamine Receptor From the Green Rice Leafhopper Nephotettix Cincticeps. Insect Biochem Mol Biol. 2020;120:103337. PubMed PMID: 32109588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and pharmacological characterization of a β-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps. AU - Xu,Gang, AU - Chang,Xue-Fei, AU - Gu,Gui-Xiang, AU - Jia,Wen-Xi, AU - Guo,Lei, AU - Huang,Jia, AU - Ye,Gong-Yin, Y1 - 2020/02/25/ PY - 2019/10/08/received PY - 2020/02/16/revised PY - 2020/02/20/accepted PY - 2020/2/29/pubmed PY - 2020/2/29/medline PY - 2020/2/29/entrez KW - Nephotettix cincticeps KW - Octopamine receptor KW - Pharmacology KW - cAMP SP - 103337 EP - 103337 JF - Insect biochemistry and molecular biology JO - Insect Biochem. Mol. Biol. VL - 120 N2 - As the counterparts of noradrenaline and adrenaline in vertebrates, octopamine (OA) regulates multiple physiological and behavioral processes in invertebrate. OA mediates its effects via binding to specific octopamine receptors (OARs). Functional and pharmacological characterization of OARs have been reported in several insects. However, little work was documented in hemipteran insects. We cloned a β-adrenergic-like OAR (NcOA2B2) from Nephotettix cincticeps. NcOA2B2 shares high similarity with members of the OA2B2 receptor class. Transcript level of NcOA2B2 varied in various tissues and was highly expressed in the leg. After heterologous expression in CHO-K1 cells, NcOA2B2 was dose-dependently activated by OA (EC50 = 2.56 nM) and tyramine (TA) (EC50 = 149 nM). Besides putative octopaminergic agonists, dopaminergic agonists and amitraz and DPMF potently activated NcOA2B2 in a dose-dependent manner. Receptor activity was blocked by potential antagonists and was most efficiently antagonized by asenapine. Phentolamine showed both antagonist and agonist effects on NcOA2B2. Our results offer the important information about molecular and pharmacological characterization of an OAR from N. cincticeps that will provide the basis for forthcoming studies on its roles in physiological processes and behaviors, and facilitate the design of novel insecticides for pest control. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/32109588/Molecular_and_pharmacological_characterization_of_a_β-adrenergic-like_octopamine_receptor_from_the_green_rice_leafhopper_Nephotettix_cincticeps L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(20)30026-6 DB - PRIME DP - Unbound Medicine ER -
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