TY - JOUR T1 - Simultaneous determination of ferulic acid, paeoniflorin, and albiflorin in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry: Application to a pharmacokinetic study of Danggui-Shaoyao-San. AU - Zhu,Heyun, AU - Guan,Jiao, AU - Zhang,Hui, AU - Chang,Sheng, AU - Wang,Liming, AU - Shi,Jianyi, AU - Feng,Bo, AU - Gu,Jingkai, Y1 - 2020/03/19/ PY - 2019/08/17/received PY - 2020/01/30/revised PY - 2020/02/21/accepted PY - 2020/3/1/pubmed PY - 2021/1/20/medline PY - 2020/3/1/entrez KW - active constituents KW - pharmacokinetics KW - tandem mass spectrometry KW - traditional Chinese medicine KW - ultra high performance liquid chromatography SP - 2053 EP - 2060 JF - Journal of separation science JO - J Sep Sci VL - 43 IS - 11 N2 - A rapid, selective, and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of ferulic acid, paeoniflorin, and albiflorin, the major active constituents of Danggui-Shaoyao-San, in rat plasma using geniposide as the internal standard. The plasma samples were processed by protein precipitation with acetonitrile, and then separated on a Shim-Pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) using gradient elution program with a mobile phase consisting of 0.1% aqueous formic acid and acetonitrile at a flow rate of 0.4 mL/min. The detection was achieved on a 3200 QTRAP mass spectrometer equipped with electrospray ionization source in negative ionization mode. Quantification was performed using multiple reaction monitoring mode by monitoring the fragmentation of m/z 192.9→134.0 for ferulic acid, m/z 525.0→120.9 for paeoniflorin, m/z 525.2→121.0 for albiflorin, and m/z 433.1→225.1 for the internal standard, respectively. The calibration curve was linear in the range of 5-2500 ng/mL for all the three analytes (r ≥ 0.9972) with the lower limit of quantitation of 5 ng/mL. The intraday and interday precisions were below 12.1% for all the analytes in terms of relative standard deviation, and the accuracy was within ±11.5% in terms of relative error. The extraction recovery, matrix effect and stability were satisfactory in rat plasma. The validated method was successfully applied to a pharmacokinetic study of ferulic acid, paeoniflorin, and albiflorin after oral administration of Danggui-Shaoyao-San to rats. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/32112520/Simultaneous_determination_of_ferulic_acid_paeoniflorin_and_albiflorin_in_rat_plasma_by_ultra_high_performance_liquid_chromatography_with_tandem_mass_spectrometry:_Application_to_a_pharmacokinetic_study_of_Danggui_Shaoyao_San_ DB - PRIME DP - Unbound Medicine ER -