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Prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products in China.
J Dairy Sci. 2020 May; 103(5):3994-4001.JD

Abstract

This study was conducted to reveal the prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products including powdered infant formula, raw milk, pasteurized milk, ultra-high-temperature milk, and cheese. Five hundred samples collected from 5 provinces in China were analyzed in overall experiments. Multilocus sequence typing, distribution of toxin genes, and antibiotic susceptibility of the isolates were analyzed. Fifty-four B. cereus strains were detected; of these, 13 isolates (26%) were from raw milk, 12 isolates (12%) from pasteurized milk, 10 isolates (10%) from cheese, 12 isolates (8%) from ultra-high-temperature milk, and 7 isolates (7%) from powdered infant formula. These isolates were divided into 24 sequence types (ST); among them, ST24, ST26, ST82, ST142, ST377, ST857, and ST1046 were the main dominant ST. The results of detection of toxin genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, bceT, hlyII, and cesB) showed that 94.4% isolates carried nheABC genes, whereas only 11.1% of the isolates contained the hblACD gene cluster. In addition, detection rates of cytK, bceT, entFM, hlyII, and cesB genes were 75.9, 77.8, 85.2, 53.7, and 11.1%, respectively. The antibiotic susceptibility test indicated that most of B. cereus isolates were resistant to ampicillin, penicillin, cefepime, cephalothin, and oxacillin, and were susceptible to gentamicin, chloramphenicol, imipenem, tetracycline, ciprofloxacin, trimethoprim-sulfamethoxazole, erythromycin, kanamycin, and cefotetan. Therefore, this study revealed the prevalence and characteristics of B. cereus isolated from dairy products in China, indicating the potential risk and contributing to the effective prevention and control of this pathogen.

Authors+Show Affiliations

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550005, China.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.Department of Agro-Processing, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh.Department of Immunology, College of Basic Medical Sciences, Shenyang Medical College, Shenyang 110034, China; Vascular Biology and Vascular Medicine Research Center, Shenyang Medical College, Shenyang 110034, China.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.The Department of Nutrition and Food Hygiene, Shenyang Medical College, Shenyang 110034, China. Electronic address: yw198269@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32113767

Citation

Zhao, Shengjuan, et al. "Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Bacillus Cereus Isolated From Dairy Products in China." Journal of Dairy Science, vol. 103, no. 5, 2020, pp. 3994-4001.
Zhao S, Chen J, Fei P, et al. Prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products in China. J Dairy Sci. 2020;103(5):3994-4001.
Zhao, S., Chen, J., Fei, P., Feng, H., Wang, Y., Ali, M. A., Li, S., Jing, H., & Yang, W. (2020). Prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products in China. Journal of Dairy Science, 103(5), 3994-4001. https://doi.org/10.3168/jds.2019-17541
Zhao S, et al. Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Bacillus Cereus Isolated From Dairy Products in China. J Dairy Sci. 2020;103(5):3994-4001. PubMed PMID: 32113767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products in China. AU - Zhao,Shengjuan, AU - Chen,Junliang, AU - Fei,Peng, AU - Feng,Hongxia, AU - Wang,Yao, AU - Ali,Md Aslam, AU - Li,Shuzhen, AU - Jing,Haonan, AU - Yang,Weiwei, Y1 - 2020/02/26/ PY - 2019/09/04/received PY - 2019/12/17/accepted PY - 2020/3/3/pubmed PY - 2020/3/3/medline PY - 2020/3/2/entrez KW - Bacillus cereus KW - characterization KW - dairy product SP - 3994 EP - 4001 JF - Journal of dairy science JO - J. Dairy Sci. VL - 103 IS - 5 N2 - This study was conducted to reveal the prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products including powdered infant formula, raw milk, pasteurized milk, ultra-high-temperature milk, and cheese. Five hundred samples collected from 5 provinces in China were analyzed in overall experiments. Multilocus sequence typing, distribution of toxin genes, and antibiotic susceptibility of the isolates were analyzed. Fifty-four B. cereus strains were detected; of these, 13 isolates (26%) were from raw milk, 12 isolates (12%) from pasteurized milk, 10 isolates (10%) from cheese, 12 isolates (8%) from ultra-high-temperature milk, and 7 isolates (7%) from powdered infant formula. These isolates were divided into 24 sequence types (ST); among them, ST24, ST26, ST82, ST142, ST377, ST857, and ST1046 were the main dominant ST. The results of detection of toxin genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, bceT, hlyII, and cesB) showed that 94.4% isolates carried nheABC genes, whereas only 11.1% of the isolates contained the hblACD gene cluster. In addition, detection rates of cytK, bceT, entFM, hlyII, and cesB genes were 75.9, 77.8, 85.2, 53.7, and 11.1%, respectively. The antibiotic susceptibility test indicated that most of B. cereus isolates were resistant to ampicillin, penicillin, cefepime, cephalothin, and oxacillin, and were susceptible to gentamicin, chloramphenicol, imipenem, tetracycline, ciprofloxacin, trimethoprim-sulfamethoxazole, erythromycin, kanamycin, and cefotetan. Therefore, this study revealed the prevalence and characteristics of B. cereus isolated from dairy products in China, indicating the potential risk and contributing to the effective prevention and control of this pathogen. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/32113767/Prevalence,_molecular_characterization,_and_antibiotic_susceptibility_of_Bacillus_cereus_isolated_from_dairy_products_in_China L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(20)30153-3 DB - PRIME DP - Unbound Medicine ER -
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