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Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum.
J Dairy Sci. 2020 May; 103(5):4013-4025.JD

Abstract

Breast milk is the main source of nutrition for infants; it contains considerable microflora that can be transmitted to the infant endogenously or by breastfeeding, and it plays an important role in the maturation and development of the immune system. In this study, we isolated and identified lactic acid bacteria (LAB) from human colostrum, and screened 2 strains with probiotic potential. The LAB isolated from 40 human colostrum samples belonged to 5 genera: Lactobacillus, Bifidobacterium, Streptococcus, Enterococcus, and Staphylococcus. We also isolated Propionibacterium and Actinomyces. We identified a total of 197 strains of LAB derived from human colostrum based on their morphology and 16S rRNA sequence, among them 8 strains of Bifidobacterium and 10 strains of Lactobacillus, including 3 Bifidobacterium species and 4 Lactobacillus species. The physiological and biochemical characteristics of strains with good probiotic characteristics were evaluated. The tolerances of some of the Bifidobacterium and Lactobacillus strains to gastrointestinal fluid and bile salts were evaluated in vitro, using the probiotic strains Bifidobacterium lactis BB12 and Lactobacillus rhamnosus GG as controls. Among them, B. lactis Probio-M8 and L. rhamnosus Probio-M9 showed survival rates of 97.25 and 78.33% after digestion for 11 h in artificial gastrointestinal juice, and they exhibited growth delays of 0.95 and 1.87 h, respectively, in 0.3% bile salts. These two strains have the potential for application as probiotics and will facilitate functional studies of probiotics in breast milk and the development of human milk-derived probiotics.

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Authors+Show Affiliations

Liu W
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Chen M
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Duo L
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Wang J
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Guo S
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Sun H
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Menghe B
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China. Electronic address: mhblg@163.com.
Zhang H
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs of China, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China. Electronic address: hepingdd@vip.sina.com.

MeSH

AnimalsBifidobacteriumBifidobacterium animalisColostrumEnterococcusFemaleHumansLactobacillalesLactobacillusPregnancyProbioticsRNA, Ribosomal, 16S

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32113772

Citation

Liu, Wenjun, et al. "Characterization of Potentially Probiotic Lactic Acid Bacteria and Bifidobacteria Isolated From Human Colostrum." Journal of Dairy Science, vol. 103, no. 5, 2020, pp. 4013-4025.
Liu W, Chen M, Duo L, et al. Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum. J Dairy Sci. 2020;103(5):4013-4025.
Liu, W., Chen, M., Duo, L., Wang, J., Guo, S., Sun, H., Menghe, B., & Zhang, H. (2020). Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum. Journal of Dairy Science, 103(5), 4013-4025. https://doi.org/10.3168/jds.2019-17602
Liu W, et al. Characterization of Potentially Probiotic Lactic Acid Bacteria and Bifidobacteria Isolated From Human Colostrum. J Dairy Sci. 2020;103(5):4013-4025. PubMed PMID: 32113772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum. AU - Liu,Wenjun, AU - Chen,Meixuan, AU - Duo,Lana, AU - Wang,Jicheng, AU - Guo,Shuai, AU - Sun,Haotian, AU - Menghe,Bilige, AU - Zhang,Heping, Y1 - 2020/02/26/ PY - 2019/9/17/received PY - 2019/12/26/accepted PY - 2020/3/3/pubmed PY - 2020/9/10/medline PY - 2020/3/2/entrez KW - artificial gastric juice KW - bile salt KW - human colostrum KW - isolation/identification KW - lactic acid bacteria SP - 4013 EP - 4025 JF - Journal of dairy science JO - J Dairy Sci VL - 103 IS - 5 N2 - Breast milk is the main source of nutrition for infants; it contains considerable microflora that can be transmitted to the infant endogenously or by breastfeeding, and it plays an important role in the maturation and development of the immune system. In this study, we isolated and identified lactic acid bacteria (LAB) from human colostrum, and screened 2 strains with probiotic potential. The LAB isolated from 40 human colostrum samples belonged to 5 genera: Lactobacillus, Bifidobacterium, Streptococcus, Enterococcus, and Staphylococcus. We also isolated Propionibacterium and Actinomyces. We identified a total of 197 strains of LAB derived from human colostrum based on their morphology and 16S rRNA sequence, among them 8 strains of Bifidobacterium and 10 strains of Lactobacillus, including 3 Bifidobacterium species and 4 Lactobacillus species. The physiological and biochemical characteristics of strains with good probiotic characteristics were evaluated. The tolerances of some of the Bifidobacterium and Lactobacillus strains to gastrointestinal fluid and bile salts were evaluated in vitro, using the probiotic strains Bifidobacterium lactis BB12 and Lactobacillus rhamnosus GG as controls. Among them, B. lactis Probio-M8 and L. rhamnosus Probio-M9 showed survival rates of 97.25 and 78.33% after digestion for 11 h in artificial gastrointestinal juice, and they exhibited growth delays of 0.95 and 1.87 h, respectively, in 0.3% bile salts. These two strains have the potential for application as probiotics and will facilitate functional studies of probiotics in breast milk and the development of human milk-derived probiotics. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/32113772/Characterization_of_potentially_probiotic_lactic_acid_bacteria_and_bifidobacteria_isolated_from_human_colostrum_ DB - PRIME DP - Unbound Medicine ER -
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