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Characterization of a PLDζ2 Homology Gene from Developing Castor Bean Endosperm.
Lipids. 2020 09; 55(5):537-548.L

Abstract

Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri-ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n-9) esterified to the sn-2 position of phosphatidylcholine (PtdCho) catalyzed by oleoyl-12-hydroxylase. PtdCho-derived diacylglycerol (DAG) is an important substrate pool for TAG synthesis, and the interconversion between PtdCho and DAG has been shown to play a critical role in channeling hydroxy fatty acids (HFA) to TAG. Although phospholipase D (PLD) has been reported to catalyze the hydrolysis of PtdCho to produce phosphatidic acid which can then be converted to DAG, its potential functions in the channeling of RA from PtdCho to DAG and the assembly of RA on TAG is largely unknown. In the present study, 11 PLD genes were identified from the Castor Bean Genome Database. Gene expression analysis indicated that RcPLD9 is expressed at relatively high levels in developing seeds compared to other plant tissues. Sequence and phylogenetic analyses revealed that RcPLD9 is a homolog of Arabidopsis PLDζ2. Overexpression of RcPLD9 in the Arabidopsis CL7 line producing C18-HFA resulted in RA content reductions in the polar lipid fraction (mainly PtdCho) and mono-HFA-TAG, but increased RA content in di-HFA-TAG. Since part of RA in di-HFA-TAG is derived from HFA-DAG, the results indicated that RcPLD9 facilitates the channeling of RA from PtdCho to DAG for its assembly on TAG in developing seeds.

Authors+Show Affiliations

CAS Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China.CAS Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China.Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.Jingdezhen University, Jingdezhen, 333000, China.Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32115716

Citation

Tian, Bo, et al. "Characterization of a PLDζ2 Homology Gene From Developing Castor Bean Endosperm." Lipids, vol. 55, no. 5, 2020, pp. 537-548.
Tian B, Sun M, Jayawardana K, et al. Characterization of a PLDζ2 Homology Gene from Developing Castor Bean Endosperm. Lipids. 2020;55(5):537-548.
Tian, B., Sun, M., Jayawardana, K., Wu, D., & Chen, G. (2020). Characterization of a PLDζ2 Homology Gene from Developing Castor Bean Endosperm. Lipids, 55(5), 537-548. https://doi.org/10.1002/lipd.12231
Tian B, et al. Characterization of a PLDζ2 Homology Gene From Developing Castor Bean Endosperm. Lipids. 2020;55(5):537-548. PubMed PMID: 32115716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a PLDζ2 Homology Gene from Developing Castor Bean Endosperm. AU - Tian,Bo, AU - Sun,Meijuan, AU - Jayawardana,Kethmi, AU - Wu,Ding, AU - Chen,Guanqun, Y1 - 2020/03/01/ PY - 2020/01/17/received PY - 2020/02/18/revised PY - 2020/02/18/accepted PY - 2020/3/3/pubmed PY - 2021/8/3/medline PY - 2020/3/3/entrez KW - Castor bean KW - Phosphatidylcholine KW - Phospholipase D KW - Ricinoleic acid KW - Triacylglycerol SP - 537 EP - 548 JF - Lipids JO - Lipids VL - 55 IS - 5 N2 - Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri-ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n-9) esterified to the sn-2 position of phosphatidylcholine (PtdCho) catalyzed by oleoyl-12-hydroxylase. PtdCho-derived diacylglycerol (DAG) is an important substrate pool for TAG synthesis, and the interconversion between PtdCho and DAG has been shown to play a critical role in channeling hydroxy fatty acids (HFA) to TAG. Although phospholipase D (PLD) has been reported to catalyze the hydrolysis of PtdCho to produce phosphatidic acid which can then be converted to DAG, its potential functions in the channeling of RA from PtdCho to DAG and the assembly of RA on TAG is largely unknown. In the present study, 11 PLD genes were identified from the Castor Bean Genome Database. Gene expression analysis indicated that RcPLD9 is expressed at relatively high levels in developing seeds compared to other plant tissues. Sequence and phylogenetic analyses revealed that RcPLD9 is a homolog of Arabidopsis PLDζ2. Overexpression of RcPLD9 in the Arabidopsis CL7 line producing C18-HFA resulted in RA content reductions in the polar lipid fraction (mainly PtdCho) and mono-HFA-TAG, but increased RA content in di-HFA-TAG. Since part of RA in di-HFA-TAG is derived from HFA-DAG, the results indicated that RcPLD9 facilitates the channeling of RA from PtdCho to DAG for its assembly on TAG in developing seeds. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/32115716/Characterization_of_a_PLDζ2_Homology_Gene_from_Developing_Castor_Bean_Endosperm_ L2 - https://doi.org/10.1002/lipd.12231 DB - PRIME DP - Unbound Medicine ER -