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Conventional detection and quantification real-time PCR of the pks-1 gene of Chaetomium globosum.
J Basic Microbiol. 2020 May; 60(5):407-414.JB

Abstract

Chaetomium globosum is known as a potential biocontrol indicator against various soil and seedborne pathogens. Precise data are necessary for population monitoring of C. globosum for its effective use in agriculture. A sequence-characterized amplified region marker has been applied for the detection of this biocontrol agent, which will help to detect C. globosum at the site of its application. Out of 17 isolates of C. globosum, only 8 isolates of C. globosum amplified a monomorphic band of 1,900 bp. C. globosum is known for producing chaetoglobosin A. The pks-1 gene is unique in C. globosum in that it is involved in chaetoglobosin A production, melanin formation, and sporulation. Real-time PCR of pks-1 was used to compare the expressions of the pks-1 gene and chaetoglobosin A biosynthesis and sporulation. It was found that the sporulation of C. globosum was associated with the levels of pks-1 gene expression; Cg2 isolate showed high expression of the pks-1 gene, 41.21%, and also produced a great number of spores and perithecia. The association between the pks-1 gene expression and chaetoglobosin A production was estimated. The Pks-1 gene was expressed by all C. globosum isolates except one isolate, C1, which is another species of Chaetomium. In addition, all C. globosum isolates produced chaetoglobosin A with different concentrations and did not express the same levels of pks-1. This finding may be a result of the solvent type used in the extraction.

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Authors+Show Affiliations

Gherbawy YA
Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, Egypt.
El-Dawy EGAE
Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, Egypt.

MeSH

ChaetomiumFungal ProteinsGene ExpressionGenetic MarkersIndole AlkaloidsReal-Time Polymerase Chain ReactionSpecies SpecificitySpores, Fungal

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32124471

Citation

Gherbawy, Youssuf A., and Eman G A E. El-Dawy. "Conventional Detection and Quantification Real-time PCR of the Pks-1 Gene of Chaetomium Globosum." Journal of Basic Microbiology, vol. 60, no. 5, 2020, pp. 407-414.
Gherbawy YA, El-Dawy EGAE. Conventional detection and quantification real-time PCR of the pks-1 gene of Chaetomium globosum. J Basic Microbiol. 2020;60(5):407-414.
Gherbawy, Y. A., & El-Dawy, E. G. A. E. (2020). Conventional detection and quantification real-time PCR of the pks-1 gene of Chaetomium globosum. Journal of Basic Microbiology, 60(5), 407-414. https://doi.org/10.1002/jobm.201900672
Gherbawy YA, El-Dawy EGAE. Conventional Detection and Quantification Real-time PCR of the Pks-1 Gene of Chaetomium Globosum. J Basic Microbiol. 2020;60(5):407-414. PubMed PMID: 32124471.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conventional detection and quantification real-time PCR of the pks-1 gene of Chaetomium globosum. AU - Gherbawy,Youssuf A, AU - El-Dawy,Eman G A E, Y1 - 2020/03/02/ PY - 2019/12/13/received PY - 2020/02/12/revised PY - 2020/02/15/accepted PY - 2020/3/4/pubmed PY - 2021/1/27/medline PY - 2020/3/4/entrez KW - Chaetomium globosum KW - SCAR marker KW - pks-1 gene SP - 407 EP - 414 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 60 IS - 5 N2 - Chaetomium globosum is known as a potential biocontrol indicator against various soil and seedborne pathogens. Precise data are necessary for population monitoring of C. globosum for its effective use in agriculture. A sequence-characterized amplified region marker has been applied for the detection of this biocontrol agent, which will help to detect C. globosum at the site of its application. Out of 17 isolates of C. globosum, only 8 isolates of C. globosum amplified a monomorphic band of 1,900 bp. C. globosum is known for producing chaetoglobosin A. The pks-1 gene is unique in C. globosum in that it is involved in chaetoglobosin A production, melanin formation, and sporulation. Real-time PCR of pks-1 was used to compare the expressions of the pks-1 gene and chaetoglobosin A biosynthesis and sporulation. It was found that the sporulation of C. globosum was associated with the levels of pks-1 gene expression; Cg2 isolate showed high expression of the pks-1 gene, 41.21%, and also produced a great number of spores and perithecia. The association between the pks-1 gene expression and chaetoglobosin A production was estimated. The Pks-1 gene was expressed by all C. globosum isolates except one isolate, C1, which is another species of Chaetomium. In addition, all C. globosum isolates produced chaetoglobosin A with different concentrations and did not express the same levels of pks-1. This finding may be a result of the solvent type used in the extraction. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/32124471/Conventional_detection_and_quantification_real_time_PCR_of_the_pks_1_gene_of_Chaetomium_globosum_ DB - PRIME DP - Unbound Medicine ER -