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Histamine receptors mediate contraction of reticular groove smooth muscle of adult goats.
Anat Histol Embryol. 2020 Jul; 49(4):440-450.AH

Abstract

The present study was conducted to evaluate the effect of histamine and to characterise its receptor subtypes in reticular groove (RG) smooth muscle of adult goats. The studies were done using floor and lip regions of RG. We used tension experiments on smooth muscle of RG isolated from adult goat for functional characterisation of H1 and H2 receptors. Western blotting and immunohistochemistry experiments were conducted for molecular characterisation of these receptors. Histamine evoked concentration-dependent contraction of isolated RG circular and longitudinal smooth muscle preparation. Pyrilamine antagonised the action of histamine. Histamine did not induce any relaxant effect on RG preparations. Additionally, cimetidine did not produce any significant effect on histamine-induced response. Non-selective histaminic receptor antagonist cyproheptadine attenuated the contraction response to histamine in the smooth muscle. Molecular characterisation and localisation of H1 and H2 receptor proteins confirmed the presence of these receptors in RG. It is most likely that histamine-induced contractile effect in RG smooth muscle of goats is mediated by H1 histaminic receptors.

Authors+Show Affiliations

Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, India.Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, India.Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, India.Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, India.Division of Pathology, Indian Veterinary Research Institute, Izatnagar, India.Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32128873

Citation

Aneesha, V A., et al. "Histamine Receptors Mediate Contraction of Reticular Groove Smooth Muscle of Adult Goats." Anatomia, Histologia, Embryologia, vol. 49, no. 4, 2020, pp. 440-450.
Aneesha VA, Gopi RP, Kumawat S, et al. Histamine receptors mediate contraction of reticular groove smooth muscle of adult goats. Anat Histol Embryol. 2020;49(4):440-450.
Aneesha, V. A., Gopi, R. P., Kumawat, S., Susanth, V. S., Vineetha, S., & Kumar, D. (2020). Histamine receptors mediate contraction of reticular groove smooth muscle of adult goats. Anatomia, Histologia, Embryologia, 49(4), 440-450. https://doi.org/10.1111/ahe.12546
Aneesha VA, et al. Histamine Receptors Mediate Contraction of Reticular Groove Smooth Muscle of Adult Goats. Anat Histol Embryol. 2020;49(4):440-450. PubMed PMID: 32128873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histamine receptors mediate contraction of reticular groove smooth muscle of adult goats. AU - Aneesha,V A, AU - Gopi,Renjith P, AU - Kumawat,Sanjay, AU - Susanth,V S, AU - Vineetha,S, AU - Kumar,Dinesh, Y1 - 2020/03/03/ PY - 2019/06/01/received PY - 2020/01/12/revised PY - 2020/02/04/accepted PY - 2020/3/5/pubmed PY - 2020/3/5/medline PY - 2020/3/5/entrez KW - adult goats KW - histamine receptors KW - reticular groove KW - smooth muscle SP - 440 EP - 450 JF - Anatomia, histologia, embryologia JO - Anat Histol Embryol VL - 49 IS - 4 N2 - The present study was conducted to evaluate the effect of histamine and to characterise its receptor subtypes in reticular groove (RG) smooth muscle of adult goats. The studies were done using floor and lip regions of RG. We used tension experiments on smooth muscle of RG isolated from adult goat for functional characterisation of H1 and H2 receptors. Western blotting and immunohistochemistry experiments were conducted for molecular characterisation of these receptors. Histamine evoked concentration-dependent contraction of isolated RG circular and longitudinal smooth muscle preparation. Pyrilamine antagonised the action of histamine. Histamine did not induce any relaxant effect on RG preparations. Additionally, cimetidine did not produce any significant effect on histamine-induced response. Non-selective histaminic receptor antagonist cyproheptadine attenuated the contraction response to histamine in the smooth muscle. Molecular characterisation and localisation of H1 and H2 receptor proteins confirmed the presence of these receptors in RG. It is most likely that histamine-induced contractile effect in RG smooth muscle of goats is mediated by H1 histaminic receptors. SN - 1439-0264 UR - https://www.unboundmedicine.com/medline/citation/32128873/Histamine_receptors_mediate_contraction_of_reticular_groove_smooth_muscle_of_adult_goats L2 - https://doi.org/10.1111/ahe.12546 DB - PRIME DP - Unbound Medicine ER -
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