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Lactobacillus enshiensis sp. nov., a novel arsenic-resistant bacterium.
Int J Syst Evol Microbiol. 2020 Apr; 70(4):2573-2580.IJ

Abstract

A lactic acid bacterial strain, HBUAS57009T, isolated from traditionally fermented food (Zha-Chili) in China, was characterized to clarify its taxonomic status using a polyphasic approach. Strain HBUAS57009T was phylogenetically closely related to Lactobacillus koreensis DCY50T, Lactobacillus fujinensis 218-6T, Lactobacillus mulengensis 112-3T, Lactobacillus cerevisiae TUM BP 140423000-2250T, Lactobacillus tongjiangensis 218-10T and Lactobacillus yonginensis THK-V8T with sequence similarities of 98.6-99.3 %. The genome-to-genome distance and average nucleotide identity values between the genomes of strain HBUAS57009T and type strains of closely related Lactobacillus species were less than 32.0 and 86.0 %, respectively; this is below the threshold for species boundaries. The major cellular fatty acids (>10 %) were C16 : 0, C18 : 1 ω9c and iso-C19 : 0. The G+C content of the genomic DNA of strain HBUAS57009T was 47.8 mol%. Examination of the functional categories of the genome revealed that strain HBUAS57009T could perform both homolactic and heterolactic fermentation processes to produce lactic acid via complete glycolysis and the pentose phosphate pathway. The putative biosynthesis pathway of butane-2,3-diol and acetoin, two important flavour compounds in the food industry, were identified using kegg mapper analysis. Based on its genotypic and phenotypic features, strain HBUAS57009T (=GDMCC 1.1664T=KACC 21424T) is designated as the type strain of a novel species, for which the name Lactobacillus enshiensis sp. nov. is proposed.

Authors+Show Affiliations

Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Huhhot, PR China.Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Huhhot, PR China.Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32129738

Citation

Zhang, Zhendong, et al. "Lactobacillus Enshiensis Sp. Nov., a Novel Arsenic-resistant Bacterium." International Journal of Systematic and Evolutionary Microbiology, vol. 70, no. 4, 2020, pp. 2573-2580.
Zhang Z, Wang Y, Hou Q, et al. Lactobacillus enshiensis sp. nov., a novel arsenic-resistant bacterium. Int J Syst Evol Microbiol. 2020;70(4):2573-2580.
Zhang, Z., Wang, Y., Hou, Q., Zhao, H., Li, W., Sun, Z., & Guo, Z. (2020). Lactobacillus enshiensis sp. nov., a novel arsenic-resistant bacterium. International Journal of Systematic and Evolutionary Microbiology, 70(4), 2573-2580. https://doi.org/10.1099/ijsem.0.004072
Zhang Z, et al. Lactobacillus Enshiensis Sp. Nov., a Novel Arsenic-resistant Bacterium. Int J Syst Evol Microbiol. 2020;70(4):2573-2580. PubMed PMID: 32129738.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lactobacillus enshiensis sp. nov., a novel arsenic-resistant bacterium. AU - Zhang,Zhendong, AU - Wang,Yurong, AU - Hou,Qiangchuan, AU - Zhao,Huijun, AU - Li,Weicheng, AU - Sun,Zhihong, AU - Guo,Zhuang, Y1 - 2020/03/04/ PY - 2020/3/5/pubmed PY - 2020/7/17/medline PY - 2020/3/5/entrez KW - Flavour compound KW - Genome KW - Lactic acid bacteria KW - Phylogeny SP - 2573 EP - 2580 JF - International journal of systematic and evolutionary microbiology JO - Int. J. Syst. Evol. Microbiol. VL - 70 IS - 4 N2 - A lactic acid bacterial strain, HBUAS57009T, isolated from traditionally fermented food (Zha-Chili) in China, was characterized to clarify its taxonomic status using a polyphasic approach. Strain HBUAS57009T was phylogenetically closely related to Lactobacillus koreensis DCY50T, Lactobacillus fujinensis 218-6T, Lactobacillus mulengensis 112-3T, Lactobacillus cerevisiae TUM BP 140423000-2250T, Lactobacillus tongjiangensis 218-10T and Lactobacillus yonginensis THK-V8T with sequence similarities of 98.6-99.3 %. The genome-to-genome distance and average nucleotide identity values between the genomes of strain HBUAS57009T and type strains of closely related Lactobacillus species were less than 32.0 and 86.0 %, respectively; this is below the threshold for species boundaries. The major cellular fatty acids (>10 %) were C16 : 0, C18 : 1 ω9c and iso-C19 : 0. The G+C content of the genomic DNA of strain HBUAS57009T was 47.8 mol%. Examination of the functional categories of the genome revealed that strain HBUAS57009T could perform both homolactic and heterolactic fermentation processes to produce lactic acid via complete glycolysis and the pentose phosphate pathway. The putative biosynthesis pathway of butane-2,3-diol and acetoin, two important flavour compounds in the food industry, were identified using kegg mapper analysis. Based on its genotypic and phenotypic features, strain HBUAS57009T (=GDMCC 1.1664T=KACC 21424T) is designated as the type strain of a novel species, for which the name Lactobacillus enshiensis sp. nov. is proposed. SN - 1466-5034 UR - https://www.unboundmedicine.com/medline/citation/32129738/Lactobacillus_enshiensis_sp._nov.,_a_novel_arsenic-resistant_bacterium L2 - http://ijs.microbiologyresearch.org/pubmed/content/journal/ijsem/10.1099/ijsem.0.004072 DB - PRIME DP - Unbound Medicine ER -