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UHPLC-ESI-HRMS/MS analysis on phenolic compositions of different E Se tea extracts and their antioxidant and cytoprotective activities.
Food Chem. 2020 Jul 15; 318:126512.FC

Abstract

E Se tea, prepared from the leaves of Malus toringoides (Rehd.) Hughes, is a traditional beverage, but there is little known about its chemical substances. This paper is aimed to investigate the chemical composition, antioxidant, and cytoprotective activities of the extract and fractions from E Se tea. Sixteen compounds were characterized by UHPLC-ESI-HRMS/MS. Phloridzin was the main compound, especially in ethyl acetate fraction (EAF). Moreover, EAF had the highest total phenolic and flavonoid contents with 197.54 ± 7.52 mg gallic acid equivalents/g extract and 85.94 ± 5.39 mg rutin equivalents/g extract, respectively, and exhibited the strongest antioxidant capacity (DPPH: IC50 = 54.91 ± 3.38 μg/mL; ABTS: IC50 = 98.08 ± 6.92 μg/mL). Different fractions of E Se tea, especially EAF, significantly inhibited intracellular ROS generation, reduced cell apoptosis, and decreased oxidative stress damage in H2O2-induced HepG-2 cells. Therefore, the obtained results highlight that E Se tea is a promising source for functional beverage or nutritional foods.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fan Z
The Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, People's Republic of China.
Wang Y
Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, People's Republic of China.
Yang M
The Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, People's Republic of China.
Cao J
The Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, People's Republic of China.
Khan A
Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan.
Cheng G
The Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, People's Republic of China. Electronic address: chengguiguang@163.com.

MeSH

AntioxidantsChromatography, High Pressure LiquidFlavonoidsHep G2 CellsHumansHydrogen PeroxideMalusOxidative StressPhenolsPlant ExtractsPlant LeavesReactive Oxygen SpeciesSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryTeas, Herbal

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32135418

Citation

Fan, Zhifeng, et al. "UHPLC-ESI-HRMS/MS Analysis On Phenolic Compositions of Different E Se Tea Extracts and Their Antioxidant and Cytoprotective Activities." Food Chemistry, vol. 318, 2020, p. 126512.
Fan Z, Wang Y, Yang M, et al. UHPLC-ESI-HRMS/MS analysis on phenolic compositions of different E Se tea extracts and their antioxidant and cytoprotective activities. Food Chem. 2020;318:126512.
Fan, Z., Wang, Y., Yang, M., Cao, J., Khan, A., & Cheng, G. (2020). UHPLC-ESI-HRMS/MS analysis on phenolic compositions of different E Se tea extracts and their antioxidant and cytoprotective activities. Food Chemistry, 318, 126512. https://doi.org/10.1016/j.foodchem.2020.126512
Fan Z, et al. UHPLC-ESI-HRMS/MS Analysis On Phenolic Compositions of Different E Se Tea Extracts and Their Antioxidant and Cytoprotective Activities. Food Chem. 2020 Jul 15;318:126512. PubMed PMID: 32135418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - UHPLC-ESI-HRMS/MS analysis on phenolic compositions of different E Se tea extracts and their antioxidant and cytoprotective activities. AU - Fan,Zhifeng, AU - Wang,Yudan, AU - Yang,Meilian, AU - Cao,Jianxin, AU - Khan,Afsar, AU - Cheng,Guiguang, Y1 - 2020/02/27/ PY - 2019/11/12/received PY - 2020/02/24/revised PY - 2020/02/26/accepted PY - 2020/3/7/pubmed PY - 2020/6/17/medline PY - 2020/3/6/entrez KW - Antioxidant KW - Aspalathin (PubChem CID: 16752601) KW - Catechin (Pubchem CID: 9064) KW - Cell apoptosis KW - Daidzin-7-glucoside (PubChem CID: 8032168) KW - Dioscin (PubChem CID: 119245) KW - E Se tea KW - Hirsutrin (PubChem CID: 5280804) KW - Hyperoside (PubChem CID: 5281643) KW - Kaempferol-3-O-glucoside (PubChem CID: 5282102) KW - Phloretin (PubChem CID: 4788) KW - Phlorizin (PubChem CID: 6072) KW - Procyanidin (PubChem CID: 107876) KW - Quercetin (PubChem CID: 5280343) KW - Quercetin-3-O-xyloside (PubChem CID: 5320863) KW - ROS KW - Rhapontin (PubChem CID: 637213) KW - UHPLC-ESI-HRMS/MS KW - p-Coumaric acid (PubChem CID: 637542) SP - 126512 EP - 126512 JF - Food chemistry JO - Food Chem VL - 318 N2 - E Se tea, prepared from the leaves of Malus toringoides (Rehd.) Hughes, is a traditional beverage, but there is little known about its chemical substances. This paper is aimed to investigate the chemical composition, antioxidant, and cytoprotective activities of the extract and fractions from E Se tea. Sixteen compounds were characterized by UHPLC-ESI-HRMS/MS. Phloridzin was the main compound, especially in ethyl acetate fraction (EAF). Moreover, EAF had the highest total phenolic and flavonoid contents with 197.54 ± 7.52 mg gallic acid equivalents/g extract and 85.94 ± 5.39 mg rutin equivalents/g extract, respectively, and exhibited the strongest antioxidant capacity (DPPH: IC50 = 54.91 ± 3.38 μg/mL; ABTS: IC50 = 98.08 ± 6.92 μg/mL). Different fractions of E Se tea, especially EAF, significantly inhibited intracellular ROS generation, reduced cell apoptosis, and decreased oxidative stress damage in H2O2-induced HepG-2 cells. Therefore, the obtained results highlight that E Se tea is a promising source for functional beverage or nutritional foods. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/32135418/UHPLC_ESI_HRMS/MS_analysis_on_phenolic_compositions_of_different_E_Se_tea_extracts_and_their_antioxidant_and_cytoprotective_activities_ DB - PRIME DP - Unbound Medicine ER -
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