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Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease.
Rice (N Y). 2020 Mar 06; 13(1):15.R

Abstract

BACKGROUND

Loose Plant Architecture 1 (LPA1), an indeterminate domain (IDD) protein, exhibits almost no expression in the leaves, but the overexpression of LPA1 significantly increases the resistance of rice to sheath blight disease (ShB) via the activation of PIN-FORMED 1a (PIN1a).

RESULTS

In this study, we determined that Rhizoctonia solani infection significantly induced LPA1 expression in the leaves, and lpa1 was more susceptible to R. solani compared with the wild-type and revertant plants. In addition, infection with R. solani altered the expression of IDD3, IDD5, IDD10, and IDD13, and yeast two-hybrid, split-GFP, and coimmunoprecipitation assays showed that LPA1 interacts with IDD3 and IDD13. IDD13 RNAi plants were more susceptible, while IDD13 overexpressors were less susceptible to ShB compared with the wild-type. In parallel, idd3 exhibited no significant differences, while IDD3 overexpressors were more susceptible compared to the wild-type response to ShB. Additional chromatin-immunoprecipitation and electrophoretic mobility shift assay experiments indicated that IDD13 and IDD3 bound to the PIN1a promoter, and the transient assay indicated that IDD13 and IDD3 positively and negatively regulate PIN1a expression, respectively. Moreover, IDD13, IDD3, and LPA1 form a transcription factor complex that regulates PIN1a. A genetic study showed that the LPA1 repressor lines were similar to lpa1/IDD13 RNAi and were more susceptible than the lpa1 and IDD13 RNAi plants in response to ShB. The overexpression of IDD13 increased resistance to ShB in the lpa1 background.

CONCLUSIONS

Taken together, our analyses established that IDD3, IDD13, and LPA1 form a transcription factor complex to regulate the defense of rice against ShB possibly via the regulation of PIN1a.

Authors+Show Affiliations

College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, China.College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.Shaanxi Key Laboratory of Chinese Jujube, Yan'an University, Yan'an, 716000, Shaanxi, China. College of Life Science, Yan'an University, Yan'an, 716000, Shaanxi, China.Microbial Research Institute, Liaoning Academy of Agricultural Sciences, Chaoyang, 122000, China.College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China. xiaohan@caas.cn.College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China. xuanyuanhu115@syau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32140886

Citation

Sun, Qian, et al. "Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease." Rice (New York, N.Y.), vol. 13, no. 1, 2020, p. 15.
Sun Q, Li DD, Chu J, et al. Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease. Rice (N Y). 2020;13(1):15.
Sun, Q., Li, D. D., Chu, J., Yuan, P., Li, S., Zhong, L. J., Han, X., & Xuan, Y. H. (2020). Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease. Rice (New York, N.Y.), 13(1), 15. https://doi.org/10.1186/s12284-020-0371-1
Sun Q, et al. Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease. Rice (N Y). 2020 Mar 6;13(1):15. PubMed PMID: 32140886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease. AU - Sun,Qian, AU - Li,Dan Dan, AU - Chu,Jin, AU - Yuan,De Peng, AU - Li,Shuang, AU - Zhong,Li Juan, AU - Han,Xiao, AU - Xuan,Yuan Hu, Y1 - 2020/03/06/ PY - 2019/10/24/received PY - 2020/01/28/accepted PY - 2020/3/7/entrez PY - 2020/3/7/pubmed PY - 2020/3/7/medline KW - Defense KW - Indeterminate domain protein KW - Rice KW - Sheath blight disease KW - Transcription activation SP - 15 EP - 15 JF - Rice (New York, N.Y.) JO - Rice (N Y) VL - 13 IS - 1 N2 - BACKGROUND: Loose Plant Architecture 1 (LPA1), an indeterminate domain (IDD) protein, exhibits almost no expression in the leaves, but the overexpression of LPA1 significantly increases the resistance of rice to sheath blight disease (ShB) via the activation of PIN-FORMED 1a (PIN1a). RESULTS: In this study, we determined that Rhizoctonia solani infection significantly induced LPA1 expression in the leaves, and lpa1 was more susceptible to R. solani compared with the wild-type and revertant plants. In addition, infection with R. solani altered the expression of IDD3, IDD5, IDD10, and IDD13, and yeast two-hybrid, split-GFP, and coimmunoprecipitation assays showed that LPA1 interacts with IDD3 and IDD13. IDD13 RNAi plants were more susceptible, while IDD13 overexpressors were less susceptible to ShB compared with the wild-type. In parallel, idd3 exhibited no significant differences, while IDD3 overexpressors were more susceptible compared to the wild-type response to ShB. Additional chromatin-immunoprecipitation and electrophoretic mobility shift assay experiments indicated that IDD13 and IDD3 bound to the PIN1a promoter, and the transient assay indicated that IDD13 and IDD3 positively and negatively regulate PIN1a expression, respectively. Moreover, IDD13, IDD3, and LPA1 form a transcription factor complex that regulates PIN1a. A genetic study showed that the LPA1 repressor lines were similar to lpa1/IDD13 RNAi and were more susceptible than the lpa1 and IDD13 RNAi plants in response to ShB. The overexpression of IDD13 increased resistance to ShB in the lpa1 background. CONCLUSIONS: Taken together, our analyses established that IDD3, IDD13, and LPA1 form a transcription factor complex to regulate the defense of rice against ShB possibly via the regulation of PIN1a. SN - 1939-8425 UR - https://www.unboundmedicine.com/medline/citation/32140886/Indeterminate_Domain_Proteins_Regulate_Rice_Defense_to_Sheath_Blight_Disease L2 - https://dx.doi.org/10.1186/s12284-020-0371-1 DB - PRIME DP - Unbound Medicine ER -
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