Effect of vedic music on steroidogenic gene expression in 3D-cultured buffalo granulosa cell spheroids model system, a pilot study.Reprod Domest Anim. 2020 Mar 07 [Online ahead of print]RD
Music is known for reducing stress, anxiety and depression, improving cognitive performance, and enhancing estrogen levels. However, its effect on non-auditory mammalian cell system and the molecular events leading to higher estrogen levels is less explored. Therefore, the present study targeted to know the direct effects of peaceful Vedic music on 3D cultured buffalo granulosa cell spheroids. The spheroids were daily exposed to the Mahamrityunjaya mantra, a kind of Vedic chants, for 1.5 h for six days. After six days, the music effect was analysed by the expression analysis of steroidogenic (CYP19A1, STAR and HSD17β1) and proliferative marker (PCNA) genes. Interestingly, the CYP19A1 gene expression was significantly up-regulated by 3.464±0.15 folds in the music exposed spheroids than the non-exposed spheroids. However, the expression of other steroidogenic and proliferative genes were unaltered. These observations provided a transcriptional clue for higher estradiol levels by the music and a scope to use Vedic chants for increasing the CYP19A1 expression to help tackle some pathophysiologcial conditions.