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[Electroacupuncture improved learning-memory ability by reducing hippocampal apoptosis and suppressing JNK signaling in rats with vascular dementia].
Zhen Ci Yan Jiu. 2020 Jan 25; 45(1):21-6.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) stimulation on the expression of c-Jun terminal kinase(JNK)signaling pathway-related proteins in the hippocampus of vascular dementia (VD) rats, so as to explore its mechanisms underlying improvement of VD.

METHODS

Male Sprague-Dawley rats were randomly divided into sham operation, model and EA groups (n=10 rats per group). The VD model was prepared by repeated occlusion of the bilateral common carotid arteries for 10 min and reperfusion for 10 min (3 times in total). The rats in the EA group received EA (2 Hz, 2 mA) at "Dazhui"(GV14),"Baihui"(GV20), and bilateral "Housanli"(ST36) ,"Geshu"(BL17) for 10 min, once daily for 14 days. The learning-memory abi-lity was detected by Morris water maze tests, the distribution of hippocampal neurons detected by Nissl staining, and the apoptosis of hippocampal neurons detected by using TdT-mediated dUTP nick-end labeling (TUNEL) method. The expressions of JNK, phosphorylated JNK (p-JNK), cysteine-containing aspartate-specific proteases-8 (Caspase-8) and Caspase-3 proteins were detected by Western blot.

RESULTS

After modeling and compared with the sham operation group, the escape latency was significantly prolonged (P<0.01) and the number of safe-platform quadrant crossing obviously decreased (P<0.01), suggesting a reduction of learning-memory ability. The number of hippocampal neurons was considerably reduced (P<0.01), and that of hippocampal apoptotic neurons remarkably increased in the model group (P<0.01). Whereas, the expression levels of hippocampal apoptosis-related proteins as JNK, p-JNK, Caspase-8 and Caspase-3, as well as the apoptotic index were significantly up-regulated (P<0.01). Following EA intervention, the learning-memory ability was apparently improved (P<0.01), and the number of hippocampal neurons was considerably increased (P<0.01), the hippocampal apoptotic cell number, apoptosis index and the expression levels of JNK, p-JNK, Caspase-8 and Caspase-3 were significantly down-regulated (P<0.01).

CONCLUSION

EA intervention can improve the learning-memory ability of VD rats, which may be associated with its effects in reducing hippocampal apoptosis by suppressing JNK signaling pathway.

Authors+Show Affiliations

College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.Department of Acupuncture and Moxibustion, Affiliated Hospital of Hebei University of Chinese Medicine, Shijiazhuang 050011.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.Research Center of Hebei University of Chinese Medicine, Shijiazhuang 050091.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.College of Acupuncture and Moxibustion, Hebei University of Chinese Medicine, Shijia-zhuang 050200, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32144904

Citation

Guo, Fei, et al. "[Electroacupuncture Improved Learning-memory Ability By Reducing Hippocampal Apoptosis and Suppressing JNK Signaling in Rats With Vascular Dementia]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 1, 2020, pp. 21-6.
Guo F, Zhang SZ, Chen SY, et al. [Electroacupuncture improved learning-memory ability by reducing hippocampal apoptosis and suppressing JNK signaling in rats with vascular dementia]. Zhen Ci Yan Jiu. 2020;45(1):21-6.
Guo, F., Zhang, S. Z., Chen, S. Y., Zhang, C., Zhang, X. Q., Gao, F., Wu, Z. H., Yu, W. T., Liang, Y. L., & Zhang, H. Z. (2020). [Electroacupuncture improved learning-memory ability by reducing hippocampal apoptosis and suppressing JNK signaling in rats with vascular dementia]. Zhen Ci Yan Jiu = Acupuncture Research, 45(1), 21-6. https://doi.org/10.13702/j.1000-0607.1905126
Guo F, et al. [Electroacupuncture Improved Learning-memory Ability By Reducing Hippocampal Apoptosis and Suppressing JNK Signaling in Rats With Vascular Dementia]. Zhen Ci Yan Jiu. 2020 Jan 25;45(1):21-6. PubMed PMID: 32144904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture improved learning-memory ability by reducing hippocampal apoptosis and suppressing JNK signaling in rats with vascular dementia]. AU - Guo,Fei, AU - Zhang,Su Zhao, AU - Chen,Shi Yu, AU - Zhang,Chuang, AU - Zhang,Xiao Qi, AU - Gao,Fei, AU - Wu,Ze Hui, AU - Yu,Wen Tao, AU - Liang,Yu Lei, AU - Zhang,Hui Zhen, PY - 2020/3/8/entrez PY - 2020/3/8/pubmed PY - 2020/3/13/medline KW - Cerebral ischemia­reperfusion KW - C­Jun terminal kinase signaling pathway KW - Electroacupuncture KW - Hippocampus KW - Learning­memory ability KW - Vascular dementia SP - 21 EP - 6 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 1 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation on the expression of c-Jun terminal kinase(JNK)signaling pathway-related proteins in the hippocampus of vascular dementia (VD) rats, so as to explore its mechanisms underlying improvement of VD. METHODS: Male Sprague-Dawley rats were randomly divided into sham operation, model and EA groups (n=10 rats per group). The VD model was prepared by repeated occlusion of the bilateral common carotid arteries for 10 min and reperfusion for 10 min (3 times in total). The rats in the EA group received EA (2 Hz, 2 mA) at "Dazhui"(GV14),"Baihui"(GV20), and bilateral "Housanli"(ST36) ,"Geshu"(BL17) for 10 min, once daily for 14 days. The learning-memory abi-lity was detected by Morris water maze tests, the distribution of hippocampal neurons detected by Nissl staining, and the apoptosis of hippocampal neurons detected by using TdT-mediated dUTP nick-end labeling (TUNEL) method. The expressions of JNK, phosphorylated JNK (p-JNK), cysteine-containing aspartate-specific proteases-8 (Caspase-8) and Caspase-3 proteins were detected by Western blot. RESULTS: After modeling and compared with the sham operation group, the escape latency was significantly prolonged (P<0.01) and the number of safe-platform quadrant crossing obviously decreased (P<0.01), suggesting a reduction of learning-memory ability. The number of hippocampal neurons was considerably reduced (P<0.01), and that of hippocampal apoptotic neurons remarkably increased in the model group (P<0.01). Whereas, the expression levels of hippocampal apoptosis-related proteins as JNK, p-JNK, Caspase-8 and Caspase-3, as well as the apoptotic index were significantly up-regulated (P<0.01). Following EA intervention, the learning-memory ability was apparently improved (P<0.01), and the number of hippocampal neurons was considerably increased (P<0.01), the hippocampal apoptotic cell number, apoptosis index and the expression levels of JNK, p-JNK, Caspase-8 and Caspase-3 were significantly down-regulated (P<0.01). CONCLUSION: EA intervention can improve the learning-memory ability of VD rats, which may be associated with its effects in reducing hippocampal apoptosis by suppressing JNK signaling pathway. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32144904/[Electroacupuncture_improved_learning_memory_ability_by_reducing_hippocampal_apoptosis_and_suppressing_JNK_signaling_in_rats_with_vascular_dementia]_ L2 - https://antibodies.cancer.gov/detail/CPTC-JUN-5 DB - PRIME DP - Unbound Medicine ER -