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Assessing the effects of Cry2Aa protein on Habrobracon hebetor (Hymenoptera: Braconidae), a parasitoid of Indian meal moth, Plodia interpunctella (lepidoptera: Pyralidae).
Ecotoxicol Environ Saf. 2020 May; 194:110380.EE

Abstract

Transgenic crops express Cry proteins exhibit high resistant to target insect pests. When we evaluate the effects of Cry proteins on the parasitoid of target insect pest via tritrophic experiments (transgenic plant-target insect pest-parasitoid) host quality of parasitoids might decrease because of insecticidal protein ingestion, this would cause host-quality mediated effects and influence the accuracy of biosafety assessment. In the current study, high dose of Cry2Aa protein was injected into the hemolymph of Plodia interpunctella by microinjection, and the hemolymph was used as the carrier to deliver Cry protein to Habrobracon hebetor, which has been previously reported as an ectoparasitoid of P. interpunctella larval, in order to avoid the "host-quality mediated effects". Results showed that injected Cry2Aa remained at high concentration and bioactive in the hemolymph of P. interpunctella parasitized by H. hebetor, the hemolymph of P. interpunctella could be used as carriers of Cry protein to H. hebetor, and high dose of Cry2Aa have no negative impacts on the development time, weight of pupa, sex ratio, adults weight (male and female), adult longevity and fecundity, and the activity of stress-related enzymes of H. hebetor. However, the hemolymph of P. interpunctella injected into Galanthus nivalis agglutinin (the positive control) showed significant negative impact on these parameters measured in the present study of H. hebetor. This indicated that Cry2Aa protein had no detrimental effects on the biological parameters of H. hebetor measured in the current study. Meanwhile, this study provides a new method for the safety evaluation of the ectoparasitoids of target pest and might be expanded to the other species of ectoparasitoids of target insects of Cry proteins in biosafety risk assessment.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Z
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: wangzj1995@126.com.
Cai W
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: wanluncai@mail.hzau.edu.cn.
Wang W
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: 2466275524@qq.com.
Zhao J
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: zhao-jing@mail.hzau.edu.cn.
Li Y
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: yifeng8547262@163.com.
Zou Y
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: zouyulan@mail.hzau.edu.cn.
Elgizawy KK
Plant Protection Department, Faculty of Agriculture, Benha University, Moshtohor, Toukh, 13736, Egypt. Electronic address: karam.elgizawy@fagr.bu.edu.eg.
Hua H
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: huahongxia@mail.hzau.edu.cn.

MeSH

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsFemaleFertilityHemolymphHemolysin ProteinsHost-Parasite InteractionsLarvaMaleMothsOryzaPest Control, BiologicalPlants, Genetically ModifiedPupaWasps

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32145528

Citation

Wang, Zhengjie, et al. "Assessing the Effects of Cry2Aa Protein On Habrobracon Hebetor (Hymenoptera: Braconidae), a Parasitoid of Indian Meal Moth, Plodia Interpunctella (lepidoptera: Pyralidae)." Ecotoxicology and Environmental Safety, vol. 194, 2020, p. 110380.
Wang Z, Cai W, Wang W, et al. Assessing the effects of Cry2Aa protein on Habrobracon hebetor (Hymenoptera: Braconidae), a parasitoid of Indian meal moth, Plodia interpunctella (lepidoptera: Pyralidae). Ecotoxicol Environ Saf. 2020;194:110380.
Wang, Z., Cai, W., Wang, W., Zhao, J., Li, Y., Zou, Y., Elgizawy, K. K., & Hua, H. (2020). Assessing the effects of Cry2Aa protein on Habrobracon hebetor (Hymenoptera: Braconidae), a parasitoid of Indian meal moth, Plodia interpunctella (lepidoptera: Pyralidae). Ecotoxicology and Environmental Safety, 194, 110380. https://doi.org/10.1016/j.ecoenv.2020.110380
Wang Z, et al. Assessing the Effects of Cry2Aa Protein On Habrobracon Hebetor (Hymenoptera: Braconidae), a Parasitoid of Indian Meal Moth, Plodia Interpunctella (lepidoptera: Pyralidae). Ecotoxicol Environ Saf. 2020;194:110380. PubMed PMID: 32145528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessing the effects of Cry2Aa protein on Habrobracon hebetor (Hymenoptera: Braconidae), a parasitoid of Indian meal moth, Plodia interpunctella (lepidoptera: Pyralidae). AU - Wang,Zhengjie, AU - Cai,Wanlun, AU - Wang,Wenjun, AU - Zhao,Jing, AU - Li,Yifeng, AU - Zou,Yulan, AU - Elgizawy,Karam Khamis, AU - Hua,Hongxia, Y1 - 2020/03/04/ PY - 2019/11/03/received PY - 2020/02/24/revised PY - 2020/02/25/accepted PY - 2020/3/8/pubmed PY - 2020/6/5/medline PY - 2020/3/8/entrez KW - Cry2Aa protein KW - Habrobracon hebetor KW - Hemolymph KW - Indian meal moth KW - Microinjection SP - 110380 EP - 110380 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 194 N2 - Transgenic crops express Cry proteins exhibit high resistant to target insect pests. When we evaluate the effects of Cry proteins on the parasitoid of target insect pest via tritrophic experiments (transgenic plant-target insect pest-parasitoid) host quality of parasitoids might decrease because of insecticidal protein ingestion, this would cause host-quality mediated effects and influence the accuracy of biosafety assessment. In the current study, high dose of Cry2Aa protein was injected into the hemolymph of Plodia interpunctella by microinjection, and the hemolymph was used as the carrier to deliver Cry protein to Habrobracon hebetor, which has been previously reported as an ectoparasitoid of P. interpunctella larval, in order to avoid the "host-quality mediated effects". Results showed that injected Cry2Aa remained at high concentration and bioactive in the hemolymph of P. interpunctella parasitized by H. hebetor, the hemolymph of P. interpunctella could be used as carriers of Cry protein to H. hebetor, and high dose of Cry2Aa have no negative impacts on the development time, weight of pupa, sex ratio, adults weight (male and female), adult longevity and fecundity, and the activity of stress-related enzymes of H. hebetor. However, the hemolymph of P. interpunctella injected into Galanthus nivalis agglutinin (the positive control) showed significant negative impact on these parameters measured in the present study of H. hebetor. This indicated that Cry2Aa protein had no detrimental effects on the biological parameters of H. hebetor measured in the current study. Meanwhile, this study provides a new method for the safety evaluation of the ectoparasitoids of target pest and might be expanded to the other species of ectoparasitoids of target insects of Cry proteins in biosafety risk assessment. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/32145528/Assessing_the_effects_of_Cry2Aa_protein_on_Habrobracon_hebetor__Hymenoptera:_Braconidae__a_parasitoid_of_Indian_meal_moth_Plodia_interpunctella__lepidoptera:_Pyralidae__ DB - PRIME DP - Unbound Medicine ER -

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