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Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea.
Korean J Parasitol. 2020 Feb; 58(1):87-91.KJ

Abstract

Gymnophallid metacercariae found in the Manila clam Ruditapes philippinarum ('Banjirak' in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with P. duboisi. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with P. duboisi and 75.7% identity with Gymnophalloides seoi deposited in GenBank. These results confirmed the presence of P. duboisi metacercariae in the Manila clam R. philippinarum in an estuary region of Gochang-gun, Korea.

Authors+Show Affiliations

Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea.Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea. Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, Seoul 03080, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32145733

Citation

Chang, Taehee, et al. "Morphological and Molecular Confirmation of Parvatrema Duboisi Metacercariae in the Manila Clam Ruditapes Philippinarum From Gochang-gun, Korea." The Korean Journal of Parasitology, vol. 58, no. 1, 2020, pp. 87-91.
Chang T, Jung BK, Shin H, et al. Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea. Korean J Parasitol. 2020;58(1):87-91.
Chang, T., Jung, B. K., Shin, H., Hong, S., Lee, J., Kim, D. G., Patarwut, L., Sohn, W. M., & Chai, J. Y. (2020). Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea. The Korean Journal of Parasitology, 58(1), 87-91. https://doi.org/10.3347/kjp.2020.58.1.87
Chang T, et al. Morphological and Molecular Confirmation of Parvatrema Duboisi Metacercariae in the Manila Clam Ruditapes Philippinarum From Gochang-gun, Korea. Korean J Parasitol. 2020;58(1):87-91. PubMed PMID: 32145733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea. AU - Chang,Taehee, AU - Jung,Bong-Kwang, AU - Shin,Hyejoo, AU - Hong,Sooji, AU - Lee,Jeonggyu, AU - Kim,Deok-Gyu, AU - Patarwut,Laddawan, AU - Sohn,Woon-Mok, AU - Chai,Jong-Yil, Y1 - 2020/02/29/ PY - 2019/09/10/received PY - 2019/10/19/accepted PY - 2020/3/9/entrez PY - 2020/3/9/pubmed PY - 2020/8/7/medline SP - 87 EP - 91 JF - The Korean journal of parasitology JO - Korean J. Parasitol. VL - 58 IS - 1 N2 - Gymnophallid metacercariae found in the Manila clam Ruditapes philippinarum ('Banjirak' in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with P. duboisi. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with P. duboisi and 75.7% identity with Gymnophalloides seoi deposited in GenBank. These results confirmed the presence of P. duboisi metacercariae in the Manila clam R. philippinarum in an estuary region of Gochang-gun, Korea. SN - 1738-0006 UR - https://www.unboundmedicine.com/medline/citation/32145733/Morphological_and_Molecular_Confirmation_of_Parvatrema_duboisi_Metacercariae_in_the_Manila_Clam_Ruditapes_philippinarum_from_Gochang_gun_Korea_ L2 - http://www.parasitol.or.kr/kjp/DOIx.php?id=10.3347/kjp.2020.58.1.87 DB - PRIME DP - Unbound Medicine ER -
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