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Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan.
J Infect Dev Ctries. 2020 02 29; 14(2):169-176.JI

Abstract

INTRODUCTION

The multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates have been increasingly reported from the Asian and African countries. The emergence of isolates with decreased susceptibility to fluoroquinolones and cephalosporins has worsened the situation. Recently, an outbreak from Sindh, Pakistan was reported caused by extensively drug-resistant (XDR) S. Typhi strains.

METHODOLOGY

In the present study, a total of 82 cases of typhoid have been investigated during 2018 from the febrile children referred to a tertiary care hospital in the population-wise largest province (Punjab) of Pakistan. S. Typhi was identified by standard microbiological techniques and isolates were characterized for antimicrobial resistance profiling and minimum inhibitory concentrations were determined. The presence of various ESBL genes in S. Typhi was confirmed by the PCR.

RESULTS

Out of the 82 isolates tested, 35 (43%) were found to be XDR; resistant to the first-line drugs. The resistance to third-generation cephalosporins was mainly mediated by extended-spectrum beta-lactamases i.e. blaTEM and blaCTX-M genes.

CONCLUSIONS

The higher prevalence of ESBL producing Salmonella typhi clinical strains raises the concern about transmission prevention and infection management in the community as well as clinical settings. Moreover, the study highlights the problem concerning the declining antibiotic arsenal for the therapeutic management of typhoid fever and the emergence and spread of XDR strains in Pakistan.

Authors+Show Affiliations

Department of Microbiology, Government College University Faisalabad, Pakistan. mian.muhsaeed@gmail.com.Department of Microbiology, Government College University Faisalabad, Pakistan. drmhrasool@gcuf.edu.pk.Department of Pathology, Allama Iqbal Medical College, Lahore, Pakistan. dr.farhanrasheed@gmail.com.Department of Microbiology, Government College University Faisalabad, Pakistan. drsaqalein@gcuf.edu.pk.Department of Microbiology, Government College University Faisalabad, Pakistan. matifnisar@gcuf.edu.pk.Department of Pathology, Allama Iqbal Medical College, Lahore, Pakistan. cancer26june@gmail.com.Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan. mohsin.nibge@gmail.com.National Institute of Health, Islamabad, Pakistan. mian.scientist@yahoo.com.National Institute of Health, Islamabad, Pakistan. maahin1@yahoo.com.Department of Microbiology, Government College University Faisalabad, Pakistan. mohsin.mic@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32146451

Citation

Saeed, Muhammad, et al. "Extended-spectrum Beta-lactamases Producing Extensively Drug-resistant Salmonella Typhi in Punjab, Pakistan." Journal of Infection in Developing Countries, vol. 14, no. 2, 2020, pp. 169-176.
Saeed M, Rasool MH, Rasheed F, et al. Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan. J Infect Dev Ctries. 2020;14(2):169-176.
Saeed, M., Rasool, M. H., Rasheed, F., Saqalein, M., Nisar, M. A., Imran, A. A., Tariq, S., Amir, A., Ikram, A., & Khurshid, M. (2020). Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan. Journal of Infection in Developing Countries, 14(2), 169-176. https://doi.org/10.3855/jidc.12049
Saeed M, et al. Extended-spectrum Beta-lactamases Producing Extensively Drug-resistant Salmonella Typhi in Punjab, Pakistan. J Infect Dev Ctries. 2020 02 29;14(2):169-176. PubMed PMID: 32146451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan. AU - Saeed,Muhammad, AU - Rasool,Muhammad Hidayat, AU - Rasheed,Farhan, AU - Saqalein,Muhammad, AU - Nisar,Muhammad Atif, AU - Imran,Ambreen Anwar, AU - Tariq,Saba, AU - Amir,Afreenish, AU - Ikram,Aamer, AU - Khurshid,Mohsin, Y1 - 2020/02/29/ PY - 2019/09/24/received PY - 2020/01/31/accepted PY - 2020/3/9/entrez PY - 2020/3/9/pubmed PY - 2021/1/26/medline KW - ESBL KW - Salmonella KW - Typhoid KW - cephalosporins SP - 169 EP - 176 JF - Journal of infection in developing countries JO - J Infect Dev Ctries VL - 14 IS - 2 N2 - INTRODUCTION: The multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates have been increasingly reported from the Asian and African countries. The emergence of isolates with decreased susceptibility to fluoroquinolones and cephalosporins has worsened the situation. Recently, an outbreak from Sindh, Pakistan was reported caused by extensively drug-resistant (XDR) S. Typhi strains. METHODOLOGY: In the present study, a total of 82 cases of typhoid have been investigated during 2018 from the febrile children referred to a tertiary care hospital in the population-wise largest province (Punjab) of Pakistan. S. Typhi was identified by standard microbiological techniques and isolates were characterized for antimicrobial resistance profiling and minimum inhibitory concentrations were determined. The presence of various ESBL genes in S. Typhi was confirmed by the PCR. RESULTS: Out of the 82 isolates tested, 35 (43%) were found to be XDR; resistant to the first-line drugs. The resistance to third-generation cephalosporins was mainly mediated by extended-spectrum beta-lactamases i.e. blaTEM and blaCTX-M genes. CONCLUSIONS: The higher prevalence of ESBL producing Salmonella typhi clinical strains raises the concern about transmission prevention and infection management in the community as well as clinical settings. Moreover, the study highlights the problem concerning the declining antibiotic arsenal for the therapeutic management of typhoid fever and the emergence and spread of XDR strains in Pakistan. SN - 1972-2680 UR - https://www.unboundmedicine.com/medline/citation/32146451/Extended_spectrum_beta_lactamases_producing_extensively_drug_resistant_Salmonella_Typhi_in_Punjab_Pakistan_ L2 - http://www.jidc.org/index.php/journal/article/view/32146451 DB - PRIME DP - Unbound Medicine ER -