Tags

Type your tag names separated by a space and hit enter

Bio-transformation of green tea infusion with tannase and its improvement on adipocyte metabolism.
Enzyme Microb Technol. 2020 Apr; 135:109496.EM

Abstract

Catechins in green tea possess various health benefits. Enzymatic treatment improves physiological activities by inducing bioconversion of catechins. Here, we investigated the effect of green tea infusion (GT) after tannase treatment, which transforms (-)-epigallocatechin gallate (EGCG) to gallic acid (GA) and (-)-epigallocatechin (EGC), on adipocyte differentiation and mature adipocyte metabolism. The optimal conditions for tannase-mediated improvement in GA and EGC yields in GT were investigated using response surface methodology. Yields of GA and EGC were 43-fold (0.43 mg/mL) and 1.66-fold higher (1.11 mg/mL), respectively, compared to GT without tannase treatment. The optimal reaction conditions for tannase-mediated biotransformation were observed on 0.54 mg mL-1 of tannase, reaction time (86.79 min), and reaction temperature at 22.59 °C. GT and tannase-treated GT (TANs) upregulated adiponectin, uncoupling protein 1, adipose triglyceride lipase, and hormone-sensitive lipase gene expression in differentiated 3T3-L1 adipocytes, with TAN inducing better effects than GT, which implies that tannase treatment improved the beneficial effect of GT on adipocyte metabolism. Thus, tannase-mediated bio-transformation is an attractive candidate for preparing GT with enhanced anti-obesity properties.

Authors+Show Affiliations

Department of Food Science & Technology, Chonnam National University, Gwangju 61186, Republic of Korea.Department of Food Science & Technology, Chonnam National University, Gwangju 61186, Republic of Korea.College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea.College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea.Department of Food Science & Technology, Chonnam National University, Gwangju 61186, Republic of Korea.Department of Food Science & Technology, Chonnam National University, Gwangju 61186, Republic of Korea.College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea. Electronic address: jooyhuh@jnu.ac.kr.College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea. Electronic address: u9897854@jnu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32146939

Citation

Kim, Hae-Soo, et al. "Bio-transformation of Green Tea Infusion With Tannase and Its Improvement On Adipocyte Metabolism." Enzyme and Microbial Technology, vol. 135, 2020, p. 109496.
Kim HS, Jeon DY, Javaid HMA, et al. Bio-transformation of green tea infusion with tannase and its improvement on adipocyte metabolism. Enzyme Microb Technol. 2020;135:109496.
Kim, H. S., Jeon, D. Y., Javaid, H. M. A., Sahar, N. E., Lee, H. N., Hong, S. J., Huh, J. Y., & Kim, Y. M. (2020). Bio-transformation of green tea infusion with tannase and its improvement on adipocyte metabolism. Enzyme and Microbial Technology, 135, 109496. https://doi.org/10.1016/j.enzmictec.2019.109496
Kim HS, et al. Bio-transformation of Green Tea Infusion With Tannase and Its Improvement On Adipocyte Metabolism. Enzyme Microb Technol. 2020;135:109496. PubMed PMID: 32146939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bio-transformation of green tea infusion with tannase and its improvement on adipocyte metabolism. AU - Kim,Hae-Soo, AU - Jeon,Do Yeon, AU - Javaid,Hafiz Muhammad Ahmad, AU - Sahar,Namood E, AU - Lee,Ha-Nul, AU - Hong,Seong-Jin, AU - Huh,Joo Young, AU - Kim,Young-Min, Y1 - 2019/12/23/ PY - 2019/10/05/received PY - 2019/12/12/revised PY - 2019/12/14/accepted PY - 2020/3/10/entrez PY - 2020/3/10/pubmed PY - 2020/10/2/medline KW - (-)-Epigallocatechin KW - (-)-Epigallocatechin gallate KW - Anti-obesity KW - Gallic acid KW - Tannase SP - 109496 EP - 109496 JF - Enzyme and microbial technology JO - Enzyme Microb Technol VL - 135 N2 - Catechins in green tea possess various health benefits. Enzymatic treatment improves physiological activities by inducing bioconversion of catechins. Here, we investigated the effect of green tea infusion (GT) after tannase treatment, which transforms (-)-epigallocatechin gallate (EGCG) to gallic acid (GA) and (-)-epigallocatechin (EGC), on adipocyte differentiation and mature adipocyte metabolism. The optimal conditions for tannase-mediated improvement in GA and EGC yields in GT were investigated using response surface methodology. Yields of GA and EGC were 43-fold (0.43 mg/mL) and 1.66-fold higher (1.11 mg/mL), respectively, compared to GT without tannase treatment. The optimal reaction conditions for tannase-mediated biotransformation were observed on 0.54 mg mL-1 of tannase, reaction time (86.79 min), and reaction temperature at 22.59 °C. GT and tannase-treated GT (TANs) upregulated adiponectin, uncoupling protein 1, adipose triglyceride lipase, and hormone-sensitive lipase gene expression in differentiated 3T3-L1 adipocytes, with TAN inducing better effects than GT, which implies that tannase treatment improved the beneficial effect of GT on adipocyte metabolism. Thus, tannase-mediated bio-transformation is an attractive candidate for preparing GT with enhanced anti-obesity properties. SN - 1879-0909 UR - https://www.unboundmedicine.com/medline/citation/32146939/Bio_transformation_of_green_tea_infusion_with_tannase_and_its_improvement_on_adipocyte_metabolism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-0229(19)30234-0 DB - PRIME DP - Unbound Medicine ER -