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Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities.
Molecules. 2020 Mar 05; 25(5)M

Abstract

Polyphenols play an important role on the health-promoting properties of humans. Plants belonging to Lamiaceae family are known as rich source of phenolic compounds. The current work aimed to evaluate the phenolic compounds, antioxidant, and anti-inflammatory activity of Elsholtzia ciliata (Thunb.) Hyl. ethanolic extracts from leaf, stem, flower, and whole herb. Twelve compounds were identified in ethanolic extracts using high-performance liquid chromatography (HPLC). The HPLC analysis revealed that chlorogenic acid, rosmarinic acid, and rutin were predominant compounds in ethanolicic extracts. Using HPLC-ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) post-column assay, avicularin, chlorogenic, and rosmarinic acids were identified as the predominant radical scavengers in all ethanolic extracts. All tested preparations significantly reduced the level of secretion of proinflammatory cytokines TNF-α, IL-6, and prostaglandin E2 induced by lipopolysaccharide treatment in mouse peritoneal macrophage cell culture. Stem and flower extracts were most efficient in reducing cytokine release, but leaf extract demonstrated stronger effect on prostaglandin E2 secretion. This is the first study exploring antioxidant efficiency by HPLC-ABTS post-column method and investigating anti-inflammatory activity of ethanolic extracts from E. ciliata different plant parts.

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Authors+Show Affiliations

Pudziuvelyte L
Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Eiveniu 4, LT-50161 Kaunas, Lithuania. Laboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu ave. 13, LT-50162 Kaunas, Lithuania.
Liaudanskas M
Laboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu ave. 13, LT-50162 Kaunas, Lithuania. Department of Pharmacognosy, Lithuanian University of Health Sciences, Eiveniu str. 4, LT-50161 Kaunas, Lithuania.
Jekabsone A
Laboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu ave. 13, LT-50162 Kaunas, Lithuania. Laboratory of Molecular Neurobiology, Neuroscience Institute, Lithuanian University of Health Sciences, Eiveniu str. 4, LT-50161 Kaunas, Lithuania.
Sadauskiene I
Laboratory of Molecular Neurobiology, Neuroscience Institute, Lithuanian University of Health Sciences, Eiveniu str. 4, LT-50161 Kaunas, Lithuania.
Bernatoniene J
Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Eiveniu 4, LT-50161 Kaunas, Lithuania. Laboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu ave. 13, LT-50162 Kaunas, Lithuania.

MeSH

AnimalsAnti-Inflammatory AgentsAntioxidantsCell SurvivalChromatography, High Pressure LiquidInflammation MediatorsLamiaceaeMacrophages, PeritonealMicePhenolsPhytochemicalsPlant Components, AerialPlant Extracts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32150805

Citation

Pudziuvelyte, Lauryna, et al. "Elsholtzia Ciliata (Thunb.) Hyl. Extracts From Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities." Molecules (Basel, Switzerland), vol. 25, no. 5, 2020.
Pudziuvelyte L, Liaudanskas M, Jekabsone A, et al. Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities. Molecules. 2020;25(5).
Pudziuvelyte, L., Liaudanskas, M., Jekabsone, A., Sadauskiene, I., & Bernatoniene, J. (2020). Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities. Molecules (Basel, Switzerland), 25(5). https://doi.org/10.3390/molecules25051153
Pudziuvelyte L, et al. Elsholtzia Ciliata (Thunb.) Hyl. Extracts From Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities. Molecules. 2020 Mar 5;25(5) PubMed PMID: 32150805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities. AU - Pudziuvelyte,Lauryna, AU - Liaudanskas,Mindaugas, AU - Jekabsone,Aiste, AU - Sadauskiene,Ilona, AU - Bernatoniene,Jurga, Y1 - 2020/03/05/ PY - 2020/01/21/received PY - 2020/02/20/revised PY - 2020/03/03/accepted PY - 2020/3/11/entrez PY - 2020/3/11/pubmed PY - 2020/12/15/medline KW - Elsholtzia ciliata KW - HPLC–ABTS post-column KW - anti-inflammatory activity KW - antioxidant KW - natural compounds KW - polyphenols KW - rosmarinic acid JF - Molecules (Basel, Switzerland) JO - Molecules VL - 25 IS - 5 N2 - Polyphenols play an important role on the health-promoting properties of humans. Plants belonging to Lamiaceae family are known as rich source of phenolic compounds. The current work aimed to evaluate the phenolic compounds, antioxidant, and anti-inflammatory activity of Elsholtzia ciliata (Thunb.) Hyl. ethanolic extracts from leaf, stem, flower, and whole herb. Twelve compounds were identified in ethanolic extracts using high-performance liquid chromatography (HPLC). The HPLC analysis revealed that chlorogenic acid, rosmarinic acid, and rutin were predominant compounds in ethanolicic extracts. Using HPLC-ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) post-column assay, avicularin, chlorogenic, and rosmarinic acids were identified as the predominant radical scavengers in all ethanolic extracts. All tested preparations significantly reduced the level of secretion of proinflammatory cytokines TNF-α, IL-6, and prostaglandin E2 induced by lipopolysaccharide treatment in mouse peritoneal macrophage cell culture. Stem and flower extracts were most efficient in reducing cytokine release, but leaf extract demonstrated stronger effect on prostaglandin E2 secretion. This is the first study exploring antioxidant efficiency by HPLC-ABTS post-column method and investigating anti-inflammatory activity of ethanolic extracts from E. ciliata different plant parts. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/32150805/Elsholtzia_ciliata__Thunb___Hyl__Extracts_from_Different_Plant_Parts:_Phenolic_Composition_Antioxidant_and_Anti_Inflammatory_Activities_ DB - PRIME DP - Unbound Medicine ER -