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Sulfur application reduces cadmium uptake in edible parts of pakchoi (Brassica chinensis L.) by cadmium chelation and vacuolar sequestration.
Ecotoxicol Environ Saf. 2020 May; 194:110402.EE

Abstract

Sulfur (S) application in pakchoi (Brassica chinensis L.) cultivation is vital for reducing cadmium (Cd) accumulation in the plants. However, the mechanism of S application on Cd uptake and translocation in pakchoi is unclear. In this study, a hydroponic experiment was performed to investigate the effects of S application on Cd accumulation in pakchoi at one Cd concentration (50 μM, in comparison to the control condition, 0 μM) and three S levels (0, 2, 4 mM). The results showed that excessive S application (4 mM) reduced Cd accumulation and alleviated pakchoi growth inhibition caused by Cd stress in shoots and roots. With increased S application, the proportion of Cd in the vacuolar fraction and the proportion of NaCl-extractable Cd increased in roots. Additionally, S application increased the content of glutathione (GSH) and phytochelatins (PCs). The reduced Cd uptake and accumulation in pakchoi shoots could have been due to increased Cd chelation and vacuolar sequestration in roots. In addition, sufficient S application (2 mM) increased the expression of γ-glutamylcysteine synthetase (GSH1) and nicotinamide synthase (NAS) in roots, and excessive S application upregulated the expression of ATP sulfurylase (ATPS) and phytochelatin synthase (PCs). This study provides evidence for the mechanism of mitigating Cd toxicity in pakchoi and will be helpful for developing strategies to reduce Cd accumulation in the edible parts of pakchoi through S fertilizer application.

Authors+Show Affiliations

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, China.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China. Electronic address: jinyinlv@nwsuaf.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32151867

Citation

Li, Hailan, et al. "Sulfur Application Reduces Cadmium Uptake in Edible Parts of Pakchoi (Brassica Chinensis L.) By Cadmium Chelation and Vacuolar Sequestration." Ecotoxicology and Environmental Safety, vol. 194, 2020, p. 110402.
Li H, Pu P, Li X, et al. Sulfur application reduces cadmium uptake in edible parts of pakchoi (Brassica chinensis L.) by cadmium chelation and vacuolar sequestration. Ecotoxicol Environ Saf. 2020;194:110402.
Li, H., Pu, P., Li, X., Gong, Y., An, D., Zhang, L., & Lv, J. (2020). Sulfur application reduces cadmium uptake in edible parts of pakchoi (Brassica chinensis L.) by cadmium chelation and vacuolar sequestration. Ecotoxicology and Environmental Safety, 194, 110402. https://doi.org/10.1016/j.ecoenv.2020.110402
Li H, et al. Sulfur Application Reduces Cadmium Uptake in Edible Parts of Pakchoi (Brassica Chinensis L.) By Cadmium Chelation and Vacuolar Sequestration. Ecotoxicol Environ Saf. 2020;194:110402. PubMed PMID: 32151867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sulfur application reduces cadmium uptake in edible parts of pakchoi (Brassica chinensis L.) by cadmium chelation and vacuolar sequestration. AU - Li,Hailan, AU - Pu,Peng, AU - Li,Xiaorui, AU - Gong,Yanzhen, AU - An,Disheng, AU - Zhang,Lixin, AU - Lv,Jinyin, Y1 - 2020/03/06/ PY - 2019/10/29/received PY - 2020/02/26/revised PY - 2020/02/26/accepted PY - 2020/3/11/pubmed PY - 2020/6/6/medline PY - 2020/3/11/entrez KW - Cadmium KW - Pakchoi KW - Subcellular fractions KW - Sulfur SP - 110402 EP - 110402 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 194 N2 - Sulfur (S) application in pakchoi (Brassica chinensis L.) cultivation is vital for reducing cadmium (Cd) accumulation in the plants. However, the mechanism of S application on Cd uptake and translocation in pakchoi is unclear. In this study, a hydroponic experiment was performed to investigate the effects of S application on Cd accumulation in pakchoi at one Cd concentration (50 μM, in comparison to the control condition, 0 μM) and three S levels (0, 2, 4 mM). The results showed that excessive S application (4 mM) reduced Cd accumulation and alleviated pakchoi growth inhibition caused by Cd stress in shoots and roots. With increased S application, the proportion of Cd in the vacuolar fraction and the proportion of NaCl-extractable Cd increased in roots. Additionally, S application increased the content of glutathione (GSH) and phytochelatins (PCs). The reduced Cd uptake and accumulation in pakchoi shoots could have been due to increased Cd chelation and vacuolar sequestration in roots. In addition, sufficient S application (2 mM) increased the expression of γ-glutamylcysteine synthetase (GSH1) and nicotinamide synthase (NAS) in roots, and excessive S application upregulated the expression of ATP sulfurylase (ATPS) and phytochelatin synthase (PCs). This study provides evidence for the mechanism of mitigating Cd toxicity in pakchoi and will be helpful for developing strategies to reduce Cd accumulation in the edible parts of pakchoi through S fertilizer application. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/32151867/Sulfur_application_reduces_cadmium_uptake_in_edible_parts_of_pakchoi__Brassica_chinensis_L___by_cadmium_chelation_and_vacuolar_sequestration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(20)30241-4 DB - PRIME DP - Unbound Medicine ER -