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Metabolic Engineering of Bacillus licheniformis for Production of Acetoin.
Front Bioeng Biotechnol. 2020; 8:125.FB

Abstract

Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productivity. In this study, B. licheniformis MW3 was metabolic engineered for acetoin production. After deleting two 2,3-butanediol dehydrogenases encoding genes budC and gdh, an engineered strain B. licheniformis MW3 (ΔbudCΔgdh) was constructed. Using fed-batch fermentation of B. licheniformis MW3 (ΔbudCΔgdh), 64.2 g/L acetoin was produced at a productivity of 2.378 g/[L h] and a yield of 0.412 g/g from 156 g/L glucose in 27 h. The fermentation process exhibited rather high productivity and yield of acetoin, indicating that B. licheniformis MW3 (ΔbudCΔgdh) might be a promising acetoin producer.

Authors+Show Affiliations

State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32154242

Citation

Lü, Chuanjuan, et al. "Metabolic Engineering of Bacillus Licheniformis for Production of Acetoin." Frontiers in Bioengineering and Biotechnology, vol. 8, 2020, p. 125.
Lü C, Ge Y, Cao M, et al. Metabolic Engineering of Bacillus licheniformis for Production of Acetoin. Front Bioeng Biotechnol. 2020;8:125.
Lü, C., Ge, Y., Cao, M., Guo, X., Liu, P., Gao, C., Xu, P., & Ma, C. (2020). Metabolic Engineering of Bacillus licheniformis for Production of Acetoin. Frontiers in Bioengineering and Biotechnology, 8, 125. https://doi.org/10.3389/fbioe.2020.00125
Lü C, et al. Metabolic Engineering of Bacillus Licheniformis for Production of Acetoin. Front Bioeng Biotechnol. 2020;8:125. PubMed PMID: 32154242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic Engineering of Bacillus licheniformis for Production of Acetoin. AU - Lü,Chuanjuan, AU - Ge,Yongsheng, AU - Cao,Menghao, AU - Guo,Xiaoting, AU - Liu,Peihai, AU - Gao,Chao, AU - Xu,Ping, AU - Ma,Cuiqing, Y1 - 2020/02/21/ PY - 2019/10/08/received PY - 2020/02/10/accepted PY - 2020/3/11/entrez PY - 2020/3/11/pubmed PY - 2020/3/11/medline KW - 2 KW - 3-butanediol KW - 3-butanediol dehydrogenase KW - Bacillus licheniformis KW - acetoin KW - metabolic engineering SP - 125 EP - 125 JF - Frontiers in bioengineering and biotechnology JO - Front Bioeng Biotechnol VL - 8 N2 - Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productivity. In this study, B. licheniformis MW3 was metabolic engineered for acetoin production. After deleting two 2,3-butanediol dehydrogenases encoding genes budC and gdh, an engineered strain B. licheniformis MW3 (ΔbudCΔgdh) was constructed. Using fed-batch fermentation of B. licheniformis MW3 (ΔbudCΔgdh), 64.2 g/L acetoin was produced at a productivity of 2.378 g/[L h] and a yield of 0.412 g/g from 156 g/L glucose in 27 h. The fermentation process exhibited rather high productivity and yield of acetoin, indicating that B. licheniformis MW3 (ΔbudCΔgdh) might be a promising acetoin producer. SN - 2296-4185 UR - https://www.unboundmedicine.com/medline/citation/32154242/Metabolic_Engineering_of_Bacillus_licheniformis_for_Production_of_Acetoin L2 - https://doi.org/10.3389/fbioe.2020.00125 DB - PRIME DP - Unbound Medicine ER -
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