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Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020.
Clin Chim Acta. 2020 Jun; 505:172-175.CC

Abstract

BACKGROUND

There's an outbreak of a novel coronavirus (SARS-CoV-2) infection since December 2019, first in China, and currently with more than 80 thousand confirmed infection globally in 29 countries till March 2, 2020. Identification, isolation and caring for patients early are essential to limit human-to-human transmission including reducing secondary infections among close contacts and health care workers, preventing transmission amplification events. The RT-PCR detection of viral nucleic acid test (NAT) was one of the most quickly established laboratory diagnosis method in a novel viral pandemic, just as in this COVID-19 outbreak.

METHODS

4880 cases that had respiratory infection symptoms or close contact with COVID-19 patients in hospital in Wuhan, China, were tested for SARS-CoV-2 infection by use of quantitative RT-PCR (qRT-PCR) on samples from the respiratory tract. Positive rates were calculated in groups divided by genders or ages.

RESULTS

The positive rate was about 38% for the total 4880 specimens. Male and older population had a significant higher positive rates. However, 57% was positive among the specimens from the Fever Clinics. Binary logistic regression analysis showed that age, not gender, was the risk factor for SARS-CoV-2 infection in fever clinics.

CONCLUSIONS

Therefore, we concluded that viral NAT played an important role in identifying SARS-CoV-2 infection.

Authors+Show Affiliations

Dept. of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.Dept. of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.Dept. State Key Laboratory of Virology (Wuhan University), College of Life Sciences of Wuhan University, Wuhan 430072, Hubei, China; Dept. Wuhan Institute of Biotechnology, Wuhan 430075, Hubei, China.Dept. of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.Dept. State Key Laboratory of Virology (Wuhan University), College of Life Sciences of Wuhan University, Wuhan 430072, Hubei, China.Dept. State Key Laboratory of Virology (Wuhan University), College of Life Sciences of Wuhan University, Wuhan 430072, Hubei, China.School of Basic Medical Sciences, Wuhan University, Wuhan 430071, Hubei, China. Electronic address: yongfeng@whu.edu.cn.Dept. of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China. Electronic address: xinchengzhu@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32156607

Citation

Liu, Rui, et al. "Positive Rate of RT-PCR Detection of SARS-CoV-2 Infection in 4880 Cases From One Hospital in Wuhan, China, From Jan to Feb 2020." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 505, 2020, pp. 172-175.
Liu R, Han H, Liu F, et al. Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020. Clin Chim Acta. 2020;505:172-175.
Liu, R., Han, H., Liu, F., Lv, Z., Wu, K., Liu, Y., Feng, Y., & Zhu, C. (2020). Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020. Clinica Chimica Acta; International Journal of Clinical Chemistry, 505, 172-175. https://doi.org/10.1016/j.cca.2020.03.009
Liu R, et al. Positive Rate of RT-PCR Detection of SARS-CoV-2 Infection in 4880 Cases From One Hospital in Wuhan, China, From Jan to Feb 2020. Clin Chim Acta. 2020;505:172-175. PubMed PMID: 32156607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020. AU - Liu,Rui, AU - Han,Huan, AU - Liu,Fang, AU - Lv,Zhihua, AU - Wu,Kailang, AU - Liu,Yingle, AU - Feng,Yong, AU - Zhu,Chengliang, Y1 - 2020/03/07/ PY - 2020/03/02/received PY - 2020/03/06/accepted PY - 2020/3/12/pubmed PY - 2020/4/18/medline PY - 2020/3/12/entrez KW - COVID-19 KW - Nucleic acid test KW - Positive rate KW - SARS-CoV-2 SP - 172 EP - 175 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 505 N2 - BACKGROUND: There's an outbreak of a novel coronavirus (SARS-CoV-2) infection since December 2019, first in China, and currently with more than 80 thousand confirmed infection globally in 29 countries till March 2, 2020. Identification, isolation and caring for patients early are essential to limit human-to-human transmission including reducing secondary infections among close contacts and health care workers, preventing transmission amplification events. The RT-PCR detection of viral nucleic acid test (NAT) was one of the most quickly established laboratory diagnosis method in a novel viral pandemic, just as in this COVID-19 outbreak. METHODS: 4880 cases that had respiratory infection symptoms or close contact with COVID-19 patients in hospital in Wuhan, China, were tested for SARS-CoV-2 infection by use of quantitative RT-PCR (qRT-PCR) on samples from the respiratory tract. Positive rates were calculated in groups divided by genders or ages. RESULTS: The positive rate was about 38% for the total 4880 specimens. Male and older population had a significant higher positive rates. However, 57% was positive among the specimens from the Fever Clinics. Binary logistic regression analysis showed that age, not gender, was the risk factor for SARS-CoV-2 infection in fever clinics. CONCLUSIONS: Therefore, we concluded that viral NAT played an important role in identifying SARS-CoV-2 infection. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/32156607/Positive_rate_of_RT_PCR_detection_of_SARS_CoV_2_infection_in_4880_cases_from_one_hospital_in_Wuhan_China_from_Jan_to_Feb_2020_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(20)30112-1 DB - PRIME DP - Unbound Medicine ER -