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Magnesium acetyltaurate protects against endothelin-1 induced RGC loss by reducing neuroinflammation in Sprague dawley rats.
Exp Eye Res. 2020 05; 194:107996.EE

Abstract

Endothelin-1 (ET-1), a potent vasoconstrictor, plays a significant role in the pathophysiology of ocular conditions like glaucoma. Glaucoma is characterized by apoptotic loss of retinal ganglion cells (RGCs) and loss of visual fields and is a leading cause of irreversible blindness. In glaucomatous eyes, retinal ischemia causes release of pro-inflammatory mediators such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α and promotes activation of transcription factors such as nuclear factor kappa B (NFKB) and c-Jun. Magnesium acetyltaurate (MgAT) has previously been shown to protect against ET-1 induced retinal and optic nerve damage. Current study investigated the mechanisms underlying these effects of MgAT, which so far remain unknown. Sprague dawley rats were intravitreally injected with ET-1 with or without pretreatment with MgAT. Seven days post-injection, retinal expression of IL-1β, IL-6, TNF-α, NFKB and c-Jun protein and genes was determined using multiplex assay, Western blot and PCR. Animals were subjected to retrograde labeling of RGCs to determine the extent of RGC survival. RGC survival was also examined using Brn3A staining. Furthermore, visual functions of rats were determined using Morris water maze. It was observed that pre-treatment with MgAT protects against ET-1 induced increase in the retinal expression of IL-1β, IL-6 and TNF-α proteins and genes. It also protected against ET-1 induced activation of NFKB and c-Jun. These effects of MgAT were associated with greater RGC survival and preservation of visual functions in rats. In conclusion, MgAT prevents ET-1 induced RGC loss and loss of visual functions by suppressing neuroinflammatory reaction in rat retinas.

Authors+Show Affiliations

Center for Neuroscience Research, Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia.School of Medicine, International Medical University, Kuala Lumpur, Malaysia. Electronic address: renuag02@gmail.com.Center for Neuroscience Research, Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia; Volgograd State Medical University, Research Centre for Innovative Medicines, Volgograd, Russian Federation; Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia.School of Medicine, International Medical University, Kuala Lumpur, Malaysia.School of Medicine, International Medical University, Kuala Lumpur, Malaysia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32156652

Citation

Nor Arfuzir, Natasha Najwa, et al. "Magnesium Acetyltaurate Protects Against Endothelin-1 Induced RGC Loss By Reducing Neuroinflammation in Sprague Dawley Rats." Experimental Eye Research, vol. 194, 2020, p. 107996.
Nor Arfuzir NN, Agarwal R, Iezhitsa I, et al. Magnesium acetyltaurate protects against endothelin-1 induced RGC loss by reducing neuroinflammation in Sprague dawley rats. Exp Eye Res. 2020;194:107996.
Nor Arfuzir, N. N., Agarwal, R., Iezhitsa, I., Agarwal, P., & Ismail, N. M. (2020). Magnesium acetyltaurate protects against endothelin-1 induced RGC loss by reducing neuroinflammation in Sprague dawley rats. Experimental Eye Research, 194, 107996. https://doi.org/10.1016/j.exer.2020.107996
Nor Arfuzir NN, et al. Magnesium Acetyltaurate Protects Against Endothelin-1 Induced RGC Loss By Reducing Neuroinflammation in Sprague Dawley Rats. Exp Eye Res. 2020;194:107996. PubMed PMID: 32156652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnesium acetyltaurate protects against endothelin-1 induced RGC loss by reducing neuroinflammation in Sprague dawley rats. AU - Nor Arfuzir,Natasha Najwa, AU - Agarwal,Renu, AU - Iezhitsa,Igor, AU - Agarwal,Puneet, AU - Ismail,Nafeeza Mohd, Y1 - 2020/03/07/ PY - 2019/11/01/received PY - 2020/02/25/revised PY - 2020/03/06/accepted PY - 2020/3/12/pubmed PY - 2020/12/16/medline PY - 2020/3/12/entrez KW - Cytokines KW - ET-1 KW - Magnesium acetyltaurate KW - Neuroinflammation KW - Object recognition KW - Retina SP - 107996 EP - 107996 JF - Experimental eye research JO - Exp Eye Res VL - 194 N2 - Endothelin-1 (ET-1), a potent vasoconstrictor, plays a significant role in the pathophysiology of ocular conditions like glaucoma. Glaucoma is characterized by apoptotic loss of retinal ganglion cells (RGCs) and loss of visual fields and is a leading cause of irreversible blindness. In glaucomatous eyes, retinal ischemia causes release of pro-inflammatory mediators such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α and promotes activation of transcription factors such as nuclear factor kappa B (NFKB) and c-Jun. Magnesium acetyltaurate (MgAT) has previously been shown to protect against ET-1 induced retinal and optic nerve damage. Current study investigated the mechanisms underlying these effects of MgAT, which so far remain unknown. Sprague dawley rats were intravitreally injected with ET-1 with or without pretreatment with MgAT. Seven days post-injection, retinal expression of IL-1β, IL-6, TNF-α, NFKB and c-Jun protein and genes was determined using multiplex assay, Western blot and PCR. Animals were subjected to retrograde labeling of RGCs to determine the extent of RGC survival. RGC survival was also examined using Brn3A staining. Furthermore, visual functions of rats were determined using Morris water maze. It was observed that pre-treatment with MgAT protects against ET-1 induced increase in the retinal expression of IL-1β, IL-6 and TNF-α proteins and genes. It also protected against ET-1 induced activation of NFKB and c-Jun. These effects of MgAT were associated with greater RGC survival and preservation of visual functions in rats. In conclusion, MgAT prevents ET-1 induced RGC loss and loss of visual functions by suppressing neuroinflammatory reaction in rat retinas. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/32156652/Magnesium_acetyltaurate_protects_against_endothelin_1_induced_RGC_loss_by_reducing_neuroinflammation_in_Sprague_dawley_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(19)30816-4 DB - PRIME DP - Unbound Medicine ER -