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Development and characterization of Triticum turgidum - Aegilops comosa and T. turgidum - Ae. markgrafii amphidiploids.
Genome. 2020 May; 63(5):263-273.G

Abstract

Aegilops comosa and Ae. markgrafii are diploid progenitors of polyploidy species of Aegilops sharing M and C genomes, respectively. Transferring valuable genes/traits from Aegilops into wheat is an alternative strategy for wheat genetic improvement. The amphidiploids between diploid species of Aegilops and tetraploid wheat can act as bridges to overcome obstacles from direct hybridization and can be developed by the union of unreduced gametes. In this study, we developed seven Triticum turgidum - Ae. comosa and two T. turgidum - Ae. markgrafii amphidiploids. The unreduced gametes mechanisms, including first-division restitution (FDR) and single-division meiosis (SDM), were observed in triploid F1 hybrids of T. turgidum - Ae. comosa (STM) and T. turgidum - Ae. markgrafii (STC). Only FDR was observed in STC hybrids, whereas FDR or both FDR and SDM were detected in the STM hybrids. All seven pairs of M chromosomes of Ae. comosa and C chromosomes of Ae. markgrafii were distinguished by fluorescent in situ hybridization (FISH) probes pSc119.2 and pTa71 combinations with pTa-535 and (CTT)12/(ACT)7, respectively. Meanwhile, the chromosomes of tetraploid wheat and diploid Aegilops parents were distinguished by the same FISH probes. The amphidiploids possessed specific valuable traits such as multiple tillers, large seed size related traits, and stripe rust resistance that could be utilized in the genetic improvement of wheat.

Authors+Show Affiliations

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu 611130, Sichuan, P.R. China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32160479

Citation

Zuo, Yuanyuan, et al. "Development and Characterization of Triticum Turgidum - Aegilops Comosa and T. Turgidum - Ae. Markgrafii Amphidiploids." Genome, vol. 63, no. 5, 2020, pp. 263-273.
Zuo Y, Xiang Q, Dai S, et al. Development and characterization of Triticum turgidum - Aegilops comosa and T. turgidum - Ae. markgrafii amphidiploids. Genome. 2020;63(5):263-273.
Zuo, Y., Xiang, Q., Dai, S., Song, Z., Bao, T., Hao, M., Zhang, L., Liu, G., Li, J., Liu, D., Wei, Y., Zheng, Y., & Yan, Z. (2020). Development and characterization of Triticum turgidum - Aegilops comosa and T. turgidum - Ae. markgrafii amphidiploids. Genome, 63(5), 263-273. https://doi.org/10.1139/gen-2019-0215
Zuo Y, et al. Development and Characterization of Triticum Turgidum - Aegilops Comosa and T. Turgidum - Ae. Markgrafii Amphidiploids. Genome. 2020;63(5):263-273. PubMed PMID: 32160479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and characterization of Triticum turgidum - Aegilops comosa and T. turgidum - Ae. markgrafii amphidiploids. AU - Zuo,Yuanyuan, AU - Xiang,Qin, AU - Dai,Shoufen, AU - Song,Zhongping, AU - Bao,Tingyu, AU - Hao,Ming, AU - Zhang,Lianquan, AU - Liu,Gang, AU - Li,Jian, AU - Liu,Dengcai, AU - Wei,Yuming, AU - Zheng,Youliang, AU - Yan,Zehong, Y1 - 2020/03/11/ PY - 2020/3/12/pubmed PY - 2021/1/13/medline PY - 2020/3/12/entrez KW - Aegilops KW - C and M genomes KW - amphidiploids KW - amphidiploïdes KW - fluorescent in situ hybridization KW - gamètes non-réduits KW - génomes C et M KW - hybridation in situ en fluorescence KW - unreduced gametes SP - 263 EP - 273 JF - Genome JO - Genome VL - 63 IS - 5 N2 - Aegilops comosa and Ae. markgrafii are diploid progenitors of polyploidy species of Aegilops sharing M and C genomes, respectively. Transferring valuable genes/traits from Aegilops into wheat is an alternative strategy for wheat genetic improvement. The amphidiploids between diploid species of Aegilops and tetraploid wheat can act as bridges to overcome obstacles from direct hybridization and can be developed by the union of unreduced gametes. In this study, we developed seven Triticum turgidum - Ae. comosa and two T. turgidum - Ae. markgrafii amphidiploids. The unreduced gametes mechanisms, including first-division restitution (FDR) and single-division meiosis (SDM), were observed in triploid F1 hybrids of T. turgidum - Ae. comosa (STM) and T. turgidum - Ae. markgrafii (STC). Only FDR was observed in STC hybrids, whereas FDR or both FDR and SDM were detected in the STM hybrids. All seven pairs of M chromosomes of Ae. comosa and C chromosomes of Ae. markgrafii were distinguished by fluorescent in situ hybridization (FISH) probes pSc119.2 and pTa71 combinations with pTa-535 and (CTT)12/(ACT)7, respectively. Meanwhile, the chromosomes of tetraploid wheat and diploid Aegilops parents were distinguished by the same FISH probes. The amphidiploids possessed specific valuable traits such as multiple tillers, large seed size related traits, and stripe rust resistance that could be utilized in the genetic improvement of wheat. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/32160479/Development_and_characterization_of_Triticum_turgidum___Aegilops_comosa_and_T__turgidum___Ae__markgrafii_amphidiploids_ L2 - https://cdnsciencepub.com/doi/10.1139/gen-2019-0215?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -