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Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach.
J Microbiol Biotechnol. 2020 Jun 28; 30(6):903-911.JM

Abstract

Addition of probiotics to yogurt with desired health benefits is gaining increasing attention. To further understand the effect of probiotic Lactobacillus plantarum on the quality and function of fermented milk, probiotic fermented milk (PFM) made with probiotic L. plantarum K25 and yogurt starter (L. delbrueckii ssp. bulgaricus and Streptococcus thermophilus) was compared with the control fermented milk (FM) made with only the yogurt starter. The probiotic strain was shown to survive well with a viable count of 7.1 ± 0.1 log CFU/g in the PFM sample after 21 days of storage at 4°C. The strain was shown to promote formation of volatiles such as acetoin and 2,3-butanediol with milk fragrance, and it did not cause post-acidification during refrigerated storage. Metabolomics analysis by GC-MS datasets coupled with multivariate statistical analysis showed that addition of L. plantarum K25 increased formation of over 20 metabolites detected in fermented milk, among which γ-aminobutyric acid was the most prominent. Together with several other metabolites with relatively high levels in fermented milk such as glyceric acid, malic acid, succinic acid, glycine, alanine, ribose, and 1,3-dihydroxyacetone, they might play important roles in the probiotic function of L. plantarum K25. Further assay of the bioactivity of the PFM sample showed significant (p < 0.05) increase of ACE inhibitory activity from 22.3% at day 1 to 49.3% at day 21 of the refrigerated storage. Therefore, probiotic L. plantarum K25 could be explored for potential application in functional dairy products.

Authors+Show Affiliations

Mengniu Dairy (Beijing) Co., Ltd., Beijing, P.R. China.Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, P.R. China.Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, P.R. China.Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32160695

Citation

Min, Zhang, et al. "Characterization and ACE Inhibitory Activity of Fermented Milk With Probiotic Lactobacillus Plantarum K25 as Analyzed By GC-MS-Based Metabolomics Approach." Journal of Microbiology and Biotechnology, vol. 30, no. 6, 2020, pp. 903-911.
Min Z, Yunyun J, Miao C, et al. Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach. J Microbiol Biotechnol. 2020;30(6):903-911.
Min, Z., Yunyun, J., Miao, C., & Zhennai, Y. (2020). Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach. Journal of Microbiology and Biotechnology, 30(6), 903-911. https://doi.org/10.4014/jmb.1911.11007
Min Z, et al. Characterization and ACE Inhibitory Activity of Fermented Milk With Probiotic Lactobacillus Plantarum K25 as Analyzed By GC-MS-Based Metabolomics Approach. J Microbiol Biotechnol. 2020 Jun 28;30(6):903-911. PubMed PMID: 32160695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and ACE Inhibitory Activity of Fermented Milk with Probiotic Lactobacillus plantarum K25 as Analyzed by GC-MS-Based Metabolomics Approach. AU - Min,Zhang, AU - Yunyun,Jiang, AU - Miao,Cai, AU - Zhennai,Yang, PY - 2019/11/05/received PY - 2020/02/27/accepted PY - 2020/3/13/pubmed PY - 2020/3/13/medline PY - 2020/3/12/entrez KW - ACE inhibition KW - L. plantarum K25 KW - fermented milk KW - metabolites SP - 903 EP - 911 JF - Journal of microbiology and biotechnology JO - J. Microbiol. Biotechnol. VL - 30 IS - 6 N2 - Addition of probiotics to yogurt with desired health benefits is gaining increasing attention. To further understand the effect of probiotic Lactobacillus plantarum on the quality and function of fermented milk, probiotic fermented milk (PFM) made with probiotic L. plantarum K25 and yogurt starter (L. delbrueckii ssp. bulgaricus and Streptococcus thermophilus) was compared with the control fermented milk (FM) made with only the yogurt starter. The probiotic strain was shown to survive well with a viable count of 7.1 ± 0.1 log CFU/g in the PFM sample after 21 days of storage at 4°C. The strain was shown to promote formation of volatiles such as acetoin and 2,3-butanediol with milk fragrance, and it did not cause post-acidification during refrigerated storage. Metabolomics analysis by GC-MS datasets coupled with multivariate statistical analysis showed that addition of L. plantarum K25 increased formation of over 20 metabolites detected in fermented milk, among which γ-aminobutyric acid was the most prominent. Together with several other metabolites with relatively high levels in fermented milk such as glyceric acid, malic acid, succinic acid, glycine, alanine, ribose, and 1,3-dihydroxyacetone, they might play important roles in the probiotic function of L. plantarum K25. Further assay of the bioactivity of the PFM sample showed significant (p < 0.05) increase of ACE inhibitory activity from 22.3% at day 1 to 49.3% at day 21 of the refrigerated storage. Therefore, probiotic L. plantarum K25 could be explored for potential application in functional dairy products. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/32160695/Characterization_and_ACE_inhibitory_activity_of_fermented_milk_with_probiotic_Lactobacillus_plantarum_K25_as_analyzed_by_GC-MS_based_metabolomics_approach L2 - http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1911.11007 DB - PRIME DP - Unbound Medicine ER -
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