Tags

Type your tag names separated by a space and hit enter

Inter-individual variability in freshwater tolerance is related to transcript level differences in gill and posterior kidney of European sea bass.
Gene. 2020 May 30; 741:144547.GENE

Abstract

Acclimation to low salinities is a vital physiological challenge for euryhaline fish as the European sea bass Dicentrarchus labrax. This species undertakes seasonal migrations towards lagoons and estuaries where a wide range of salinity variations occur along the year. We have previously reported intraspecific differences in freshwater tolerance, with an average 30% mortality rate. In this study, we bring new evidence of mechanisms underlying freshwater tolerance in sea bass at gill and kidney levels. In fresh water (FW), intraspecific differences in mRNA expression levels of several ion transporters and prolactin receptors were measured. We showed that the branchial Cl-/HCO3- anion transporter (slc26a6c) was over-expressed in freshwater intolerant fish, probably as a compensatory response to low blood chloride levels and potential metabolic alkalosis. Moreover, prolactin receptor a (prlra) and Na+/Cl- cotransporter (ncc1) but not ncc-2a expression seemed to be slightly increased and highly variable between individuals in freshwater intolerant fish. In the posterior kidney, freshwater intolerant fish exhibited differential expression levels of slc26 anion transporters and Na+/K+/2Cl- cotransporter 1b (nkcc1b). Lower expression levels of prolactin receptors (prlra, prlrb) were measured in posterior kidney which probably contributes to the failure in ion reuptake at the kidney level. Freshwater intolerance seems to be a consequence of renal failure of ion reabsorption, which is not sufficiently compensated at the branchial level.

Authors+Show Affiliations

Univ Montpellier, MARBEC (CNRS, IFREMER, IRD, UM), Montpellier, France.Univ Montpellier, MARBEC (CNRS, IFREMER, IRD, UM), Montpellier, France.Univ Montpellier, MARBEC (CNRS, IFREMER, IRD, UM), Montpellier, France.Univ Montpellier, MARBEC (CNRS, IFREMER, IRD, UM), Montpellier, France. Electronic address: catherine.lorin@umontpellier.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32165299

Citation

L'Honoré, Thibaut, et al. "Inter-individual Variability in Freshwater Tolerance Is Related to Transcript Level Differences in Gill and Posterior Kidney of European Sea Bass." Gene, vol. 741, 2020, p. 144547.
L'Honoré T, Farcy E, Blondeau-Bidet E, et al. Inter-individual variability in freshwater tolerance is related to transcript level differences in gill and posterior kidney of European sea bass. Gene. 2020;741:144547.
L'Honoré, T., Farcy, E., Blondeau-Bidet, E., & Lorin-Nebel, C. (2020). Inter-individual variability in freshwater tolerance is related to transcript level differences in gill and posterior kidney of European sea bass. Gene, 741, 144547. https://doi.org/10.1016/j.gene.2020.144547
L'Honoré T, et al. Inter-individual Variability in Freshwater Tolerance Is Related to Transcript Level Differences in Gill and Posterior Kidney of European Sea Bass. Gene. 2020 May 30;741:144547. PubMed PMID: 32165299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inter-individual variability in freshwater tolerance is related to transcript level differences in gill and posterior kidney of European sea bass. AU - L'Honoré,Thibaut, AU - Farcy,Emilie, AU - Blondeau-Bidet,Eva, AU - Lorin-Nebel,Catherine, Y1 - 2020/03/09/ PY - 2019/09/10/received PY - 2020/01/30/revised PY - 2020/03/08/accepted PY - 2020/3/14/pubmed PY - 2020/4/25/medline PY - 2020/3/14/entrez KW - Freshwater acclimation KW - Intraspecific variation KW - Ion transporters KW - NKCC KW - Osmoregulation KW - PRLR KW - SLC26A6 SP - 144547 EP - 144547 JF - Gene JO - Gene VL - 741 N2 - Acclimation to low salinities is a vital physiological challenge for euryhaline fish as the European sea bass Dicentrarchus labrax. This species undertakes seasonal migrations towards lagoons and estuaries where a wide range of salinity variations occur along the year. We have previously reported intraspecific differences in freshwater tolerance, with an average 30% mortality rate. In this study, we bring new evidence of mechanisms underlying freshwater tolerance in sea bass at gill and kidney levels. In fresh water (FW), intraspecific differences in mRNA expression levels of several ion transporters and prolactin receptors were measured. We showed that the branchial Cl-/HCO3- anion transporter (slc26a6c) was over-expressed in freshwater intolerant fish, probably as a compensatory response to low blood chloride levels and potential metabolic alkalosis. Moreover, prolactin receptor a (prlra) and Na+/Cl- cotransporter (ncc1) but not ncc-2a expression seemed to be slightly increased and highly variable between individuals in freshwater intolerant fish. In the posterior kidney, freshwater intolerant fish exhibited differential expression levels of slc26 anion transporters and Na+/K+/2Cl- cotransporter 1b (nkcc1b). Lower expression levels of prolactin receptors (prlra, prlrb) were measured in posterior kidney which probably contributes to the failure in ion reuptake at the kidney level. Freshwater intolerance seems to be a consequence of renal failure of ion reabsorption, which is not sufficiently compensated at the branchial level. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/32165299/Inter_individual_variability_in_freshwater_tolerance_is_related_to_transcript_level_differences_in_gill_and_posterior_kidney_of_European_sea_bass_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(20)30216-X DB - PRIME DP - Unbound Medicine ER -