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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Proc Natl Acad Sci U S A. 2020 03 31; 117(13):7001-7003.PN

Abstract

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

Authors+Show Affiliations

Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32165541

Citation

Matsuyama, Shutoku, et al. "Enhanced Isolation of SARS-CoV-2 By TMPRSS2-expressing Cells." Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 13, 2020, pp. 7001-7003.
Matsuyama S, Nao N, Shirato K, et al. Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. Proc Natl Acad Sci U S A. 2020;117(13):7001-7003.
Matsuyama, S., Nao, N., Shirato, K., Kawase, M., Saito, S., Takayama, I., Nagata, N., Sekizuka, T., Katoh, H., Kato, F., Sakata, M., Tahara, M., Kutsuna, S., Ohmagari, N., Kuroda, M., Suzuki, T., Kageyama, T., & Takeda, M. (2020). Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. Proceedings of the National Academy of Sciences of the United States of America, 117(13), 7001-7003. https://doi.org/10.1073/pnas.2002589117
Matsuyama S, et al. Enhanced Isolation of SARS-CoV-2 By TMPRSS2-expressing Cells. Proc Natl Acad Sci U S A. 2020 03 31;117(13):7001-7003. PubMed PMID: 32165541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. AU - Matsuyama,Shutoku, AU - Nao,Naganori, AU - Shirato,Kazuya, AU - Kawase,Miyuki, AU - Saito,Shinji, AU - Takayama,Ikuyo, AU - Nagata,Noriyo, AU - Sekizuka,Tsuyoshi, AU - Katoh,Hiroshi, AU - Kato,Fumihiro, AU - Sakata,Masafumi, AU - Tahara,Maino, AU - Kutsuna,Satoshi, AU - Ohmagari,Norio, AU - Kuroda,Makoto, AU - Suzuki,Tadaki, AU - Kageyama,Tsutomu, AU - Takeda,Makoto, Y1 - 2020/03/12/ PY - 2020/3/14/pubmed PY - 2020/4/18/medline PY - 2020/3/14/entrez KW - SARS-CoV-2 KW - TMPRSS2 KW - VeroE6 KW - coronavirus KW - outbreak SP - 7001 EP - 7003 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 117 IS - 13 N2 - A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/32165541/Enhanced_isolation_of_SARS_CoV_2_by_TMPRSS2_expressing_cells_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=32165541 DB - PRIME DP - Unbound Medicine ER -