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Effect of soybean peptides against hydrogen peroxide induced oxidative stress in HepG2 cells via Nrf2 signaling.
Food Funct. 2020 Mar 01; 11(3):2725-2737.FF

Abstract

The aim of this study was to determine the effects of soybean protein hydrolysates against intracellular antioxidant activity. Soybean peptides (1000 to 2000 Da range) were extracted by soybean proteolysis and ultrafiltration and sequenced with a Nano-LC-ESI-MS/MS. In this study we found that soybean peptides inhibited the production of reactive oxygen species (ROS) induced by hydrogen peroxide (H2O2), malondialdehyde (MDA) and oxidized glutathione (GSSG) in HepG2 cells. Moreover, they also prevented the reduction of reduced glutathione (GSH) and up-regulated cellular resistance oxidase activity. In addition, soybean peptide treatment stimulated the mRNA and protein expression levels of antioxidant enzymes and nuclear factor erythroid-2-related factor 2 (Nrf2). Activated Nrf2 up-regulated antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)) and inhibited ROS and MDA production. It was concluded that soybean peptides effectively activated the Nrf2/antioxidant response element (ARE) mediated activity.

Authors+Show Affiliations

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), Beijing 100048, China. zhaofen1320@163.com liuxinqi@btbu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32167099

Citation

Yi, Guofu, et al. "Effect of Soybean Peptides Against Hydrogen Peroxide Induced Oxidative Stress in HepG2 Cells Via Nrf2 Signaling." Food & Function, vol. 11, no. 3, 2020, pp. 2725-2737.
Yi G, Din JU, Zhao F, et al. Effect of soybean peptides against hydrogen peroxide induced oxidative stress in HepG2 cells via Nrf2 signaling. Food Funct. 2020;11(3):2725-2737.
Yi, G., Din, J. U., Zhao, F., & Liu, X. (2020). Effect of soybean peptides against hydrogen peroxide induced oxidative stress in HepG2 cells via Nrf2 signaling. Food & Function, 11(3), 2725-2737. https://doi.org/10.1039/c9fo01466g
Yi G, et al. Effect of Soybean Peptides Against Hydrogen Peroxide Induced Oxidative Stress in HepG2 Cells Via Nrf2 Signaling. Food Funct. 2020 Mar 1;11(3):2725-2737. PubMed PMID: 32167099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of soybean peptides against hydrogen peroxide induced oxidative stress in HepG2 cells via Nrf2 signaling. AU - Yi,Guofu, AU - Din,Jalal Ud, AU - Zhao,Fen, AU - Liu,Xinqi, Y1 - 2020/03/13/ PY - 2020/3/14/pubmed PY - 2020/3/14/medline PY - 2020/3/14/entrez SP - 2725 EP - 2737 JF - Food & function JO - Food Funct VL - 11 IS - 3 N2 - The aim of this study was to determine the effects of soybean protein hydrolysates against intracellular antioxidant activity. Soybean peptides (1000 to 2000 Da range) were extracted by soybean proteolysis and ultrafiltration and sequenced with a Nano-LC-ESI-MS/MS. In this study we found that soybean peptides inhibited the production of reactive oxygen species (ROS) induced by hydrogen peroxide (H2O2), malondialdehyde (MDA) and oxidized glutathione (GSSG) in HepG2 cells. Moreover, they also prevented the reduction of reduced glutathione (GSH) and up-regulated cellular resistance oxidase activity. In addition, soybean peptide treatment stimulated the mRNA and protein expression levels of antioxidant enzymes and nuclear factor erythroid-2-related factor 2 (Nrf2). Activated Nrf2 up-regulated antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)) and inhibited ROS and MDA production. It was concluded that soybean peptides effectively activated the Nrf2/antioxidant response element (ARE) mediated activity. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/32167099/Effect_of_soybean_peptides_against_hydrogen_peroxide_induced_oxidative_stress_in_HepG2_cells_via_Nrf2_signaling_ L2 - https://doi.org/10.1039/c9fo01466g DB - PRIME DP - Unbound Medicine ER -