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Nitro-oxidative signalling induced by chemically synthetized zinc oxide nanoparticles (ZnO NPs) in Brassica species.
Chemosphere. 2020 Jul; 251:126419.C

Abstract

Due to their release into the environment, zinc oxide nanoparticles (ZnO NPs) may come in contact with plants. In elevated concentrations, ZnO NPs induce reactive oxygen species (ROS) production, but the metabolism of reactive nitrogen species (RNS) and the consequent nitro-oxidative signalling has not been examined so far. In this work, Brassica napus and Brassica juncea seedlings were treated with chemically synthetized ZnO NPs (∼8 nm, 0, 25 or 100 mg/L). At low dose (25 mg/L) ZnO NP exerted a positive effect, while at elevated concentration (100 mg/L) it was toxic to both species. Additionally, B. juncea was more tolerant to ZnO NPs than B. napus. The ZnO NPs could enter the root cells due to their small (∼8 nm) size which resulted in the release of Zn2+ and subsequently increased Zn2+ content in the plant organs. ZnO NPs disturbed superoxide radical and hydrogen peroxide homeostasis and modulated ROS metabolic enzymes (NADPH oxidase, superoxide dismutase, ascorbate peroxidase) and non-enzymatic antioxidants (ascorbate and glutathione) inducing similar changes in oxidative signalling in both Brassica species. The homeostasis of RNS (nitric oxide, peroxynitrite and S-nitrosoglutathione) was also altered by ZnO NPs; however, changes in nitrosative signalling proved to be different in the examined species. Moreover, ZnO NPs triggered changes in protein carbonylation and nitration. These results suggest that ZnO NPs induce changes in nitro-oxidative signalling which may contribute to ZnO NP toxicity. Furthermore, difference in ZnO NP tolerance of Brassica species is more likely related to nitrosative than to oxidative signalling.

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Authors+Show Affiliations

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Zoltán Kovács D

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720, Szeged, Rerrich Bela ter 1., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Pathology, Faculty of Medicine, University of Szeged, H-6725, Szeged, Állomás u. 2., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary.

Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720, Szeged, Rerrich Bela ter 1., Hungary.

Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, H-6720, Szeged, Rerrich Bela ter 1., Hungary.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, H-6726, Szeged, Közép fasor 52., Hungary. Electronic address: kolzsu@bio.u-szeged.hu.

MeSH

AntioxidantsAscorbate PeroxidasesBrassicaBrassica napusGlutathioneMustard PlantNanoparticlesOxidation-ReductionPlant RootsReactive Nitrogen SpeciesReactive Oxygen SpeciesSeedlingsSignal TransductionSuperoxide DismutaseZincZinc Oxide

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32171133

Citation

Molnár, Árpád, et al. "Nitro-oxidative Signalling Induced By Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species." Chemosphere, vol. 251, 2020, p. 126419.

Molnár Á, Papp M, Zoltán Kovács D, et al. Nitro-oxidative signalling induced by chemically synthetized zinc oxide nanoparticles (ZnO NPs) in Brassica species. Chemosphere. 2020;251:126419.

Molnár, Á., Papp, M., Zoltán Kovács, D., Bélteky, P., Oláh, D., Feigl, G., Szőllősi, R., Rázga, Z., Ördög, A., Erdei, L., Rónavári, A., Kónya, Z., & Kolbert, Z. (2020). Nitro-oxidative signalling induced by chemically synthetized zinc oxide nanoparticles (ZnO NPs) in Brassica species. Chemosphere, 251, 126419. https://doi.org/10.1016/j.chemosphere.2020.126419

Molnár Á, et al. Nitro-oxidative Signalling Induced By Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species. Chemosphere. 2020;251:126419. PubMed PMID: 32171133.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Nitro-oxidative signalling induced by chemically synthetized zinc oxide nanoparticles (ZnO NPs) in Brassica species. AU - Molnár,Árpád, AU - Papp,Márk, AU - Zoltán Kovács,Dávid, AU - Bélteky,Péter, AU - Oláh,Dóra, AU - Feigl,Gábor, AU - Szőllősi,Réka, AU - Rázga,Zsolt, AU - Ördög,Attila, AU - Erdei,László, AU - Rónavári,Andrea, AU - Kónya,Zoltán, AU - Kolbert,Zsuzsanna, Y1 - 2020/03/06/ PY - 2020/01/31/received PY - 2020/02/24/revised PY - 2020/03/03/accepted PY - 2020/3/15/pubmed PY - 2020/6/4/medline PY - 2020/3/15/entrez KW - Brassica juncea KW - Brassica napus KW - Nitro-oxidative signalling KW - Zinc oxide nanoparticle SP - 126419 EP - 126419 JF - Chemosphere JO - Chemosphere VL - 251 N2 - Due to their release into the environment, zinc oxide nanoparticles (ZnO NPs) may come in contact with plants. In elevated concentrations, ZnO NPs induce reactive oxygen species (ROS) production, but the metabolism of reactive nitrogen species (RNS) and the consequent nitro-oxidative signalling has not been examined so far. In this work, Brassica napus and Brassica juncea seedlings were treated with chemically synthetized ZnO NPs (∼8 nm, 0, 25 or 100 mg/L). At low dose (25 mg/L) ZnO NP exerted a positive effect, while at elevated concentration (100 mg/L) it was toxic to both species. Additionally, B. juncea was more tolerant to ZnO NPs than B. napus. The ZnO NPs could enter the root cells due to their small (∼8 nm) size which resulted in the release of Zn2+ and subsequently increased Zn2+ content in the plant organs. ZnO NPs disturbed superoxide radical and hydrogen peroxide homeostasis and modulated ROS metabolic enzymes (NADPH oxidase, superoxide dismutase, ascorbate peroxidase) and non-enzymatic antioxidants (ascorbate and glutathione) inducing similar changes in oxidative signalling in both Brassica species. The homeostasis of RNS (nitric oxide, peroxynitrite and S-nitrosoglutathione) was also altered by ZnO NPs; however, changes in nitrosative signalling proved to be different in the examined species. Moreover, ZnO NPs triggered changes in protein carbonylation and nitration. These results suggest that ZnO NPs induce changes in nitro-oxidative signalling which may contribute to ZnO NP toxicity. Furthermore, difference in ZnO NP tolerance of Brassica species is more likely related to nitrosative than to oxidative signalling. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/32171133/Nitro_oxidative_signalling_induced_by_chemically_synthetized_zinc_oxide_nanoparticles__ZnO_NPs__in_Brassica_species_ DB - PRIME DP - Unbound Medicine ER -