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Surface enhanced Raman spectroscopy as a novel tool for rapid quantification of heroin and metabolites in saliva
Turk J Med Sci. 2020 08 26; 50(5):1470-1479.TJ

Abstract

Background

Heroin can be detected and quantified by certain analytical methods, however, forensic professionals and criminal laboratories study for cheaper and faster detection tools. Surface-enhanced Raman spectroscopy (SERS) rises as a possible alternative tool with its widening application spectra. There are few studies regarding Raman and SERS spectra of heroin and its metabolites, which are unfortunately controversial. In this study, we compared five different surfaces in order to find out more efficient Raman-active substrate for opiate detection and rapid quantification of heroin and its metabolites in saliva.

Materials and methods

Morphine standard material was used to identify proper surface for SERS analysis of opiates. Heroin and its metabolites (morphine, morphine-3-β-glucuronide and 6-monoacetyl morphine) were calibrated between 50 ppb and 500 ppm and quantified on AuNRs with signal enhancement of silver colloids in saliva. Raman microscope with a 785-nm laser source was used.

Results and Conclusion

Obtained results showed that heroin and its metabolites can be detected and quantified in saliva samples using a SERS-based system. Additionally, the present study revealed that synergetic effect of a specific gold nano-surface with ability controlling liquid motion and silver nanoparticles increase band numbers and intensities. Therefore, we suggest a fast, accurate and cost-effective method to detect and quantify heroin in biological fluids

Authors+Show Affiliations

Department of Forensic Medicine, Faculty of Medicine, Hacettepe University, Ankara, TurkeyDepartment of Forensic Medicine, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, TurkeyDepartment of Nanotechnology and Nanomedicine, Graduate School of Science and Engineering, Hacettepe University, Ankara, TurkeyDepartment of Food Engineering, Faculty of Engineering, Hacettepe University, Ankara, TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara, TurkeyDepartment of Nanotechnology and Nanomedicine, Graduate School of Science and Engineering, Hacettepe University, Ankara, Turkey

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32178510

Citation

Akçan, Ramazan, et al. "Surface Enhanced Raman Spectroscopy as a Novel Tool for Rapid Quantification of Heroin and Metabolites in Saliva." Turkish Journal of Medical Sciences, vol. 50, no. 5, 2020, pp. 1470-1479.
Akçan R, Yildirim MŞ, Ilhan H, et al. Surface enhanced Raman spectroscopy as a novel tool for rapid quantification of heroin and metabolites in saliva. Turk J Med Sci. 2020;50(5):1470-1479.
Akçan, R., Yildirim, M. Ş., Ilhan, H., Güven, B., Tamer, U., & Sağlam, N. (2020). Surface enhanced Raman spectroscopy as a novel tool for rapid quantification of heroin and metabolites in saliva. Turkish Journal of Medical Sciences, 50(5), 1470-1479. https://doi.org/10.3906/sag-1912-196
Akçan R, et al. Surface Enhanced Raman Spectroscopy as a Novel Tool for Rapid Quantification of Heroin and Metabolites in Saliva. Turk J Med Sci. 2020 08 26;50(5):1470-1479. PubMed PMID: 32178510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface enhanced Raman spectroscopy as a novel tool for rapid quantification of heroin and metabolites in saliva AU - Akçan,Ramazan, AU - Yildirim,Mahmut Şerif, AU - Ilhan,Hasan, AU - Güven,Burcu, AU - Tamer,Uğur, AU - Sağlam,Necdet, Y1 - 2020/08/26/ PY - 2019/12/23/received PY - 2020/03/14/accepted PY - 2020/3/18/entrez PY - 2020/3/18/pubmed PY - 2021/7/13/medline KW - Heroin and morphine identification KW - Heroin and morphine quantification KW - Raman-active surface KW - gold nanorod arrays KW - surface-enhanced Raman spectroscopy (SERS) KW - silver nanoparticles SP - 1470 EP - 1479 JF - Turkish journal of medical sciences JO - Turk J Med Sci VL - 50 IS - 5 N2 - Background: Heroin can be detected and quantified by certain analytical methods, however, forensic professionals and criminal laboratories study for cheaper and faster detection tools. Surface-enhanced Raman spectroscopy (SERS) rises as a possible alternative tool with its widening application spectra. There are few studies regarding Raman and SERS spectra of heroin and its metabolites, which are unfortunately controversial. In this study, we compared five different surfaces in order to find out more efficient Raman-active substrate for opiate detection and rapid quantification of heroin and its metabolites in saliva. Materials and methods: Morphine standard material was used to identify proper surface for SERS analysis of opiates. Heroin and its metabolites (morphine, morphine-3-β-glucuronide and 6-monoacetyl morphine) were calibrated between 50 ppb and 500 ppm and quantified on AuNRs with signal enhancement of silver colloids in saliva. Raman microscope with a 785-nm laser source was used. Results and Conclusion: Obtained results showed that heroin and its metabolites can be detected and quantified in saliva samples using a SERS-based system. Additionally, the present study revealed that synergetic effect of a specific gold nano-surface with ability controlling liquid motion and silver nanoparticles increase band numbers and intensities. Therefore, we suggest a fast, accurate and cost-effective method to detect and quantify heroin in biological fluids SN - 1303-6165 UR - https://www.unboundmedicine.com/medline/citation/32178510/Surface_enhanced_Raman_spectroscopy_as_a_novel_tool_for_rapid_quantification_of_heroin_and_metabolites_in_saliva L2 - https://doi.org/10.3906/sag-1912-196 DB - PRIME DP - Unbound Medicine ER -