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Comparison of Methods for Measuring Protein Concentration in Venom Samples.
Animals (Basel). 2020 Mar 08; 10(3)A

Abstract

Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.

Authors+Show Affiliations

Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland.Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland.Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland.Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland.Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland.Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland.Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia. Zoological Department, Zoological Garden Košice, Široká 31, 040 06 Košice-Kavečany, Slovakia.Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia.Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszów, Poland. Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32182656

Citation

Bocian, Aleksandra, et al. "Comparison of Methods for Measuring Protein Concentration in Venom Samples." Animals : an Open Access Journal From MDPI, vol. 10, no. 3, 2020.
Bocian A, Sławek S, Jaromin M, et al. Comparison of Methods for Measuring Protein Concentration in Venom Samples. Animals (Basel). 2020;10(3).
Bocian, A., Sławek, S., Jaromin, M., Hus, K. K., Buczkowicz, J., Łysiak, D., Petrílla, V., Petrillova, M., & Legáth, J. (2020). Comparison of Methods for Measuring Protein Concentration in Venom Samples. Animals : an Open Access Journal From MDPI, 10(3). https://doi.org/10.3390/ani10030448
Bocian A, et al. Comparison of Methods for Measuring Protein Concentration in Venom Samples. Animals (Basel). 2020 Mar 8;10(3) PubMed PMID: 32182656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Methods for Measuring Protein Concentration in Venom Samples. AU - Bocian,Aleksandra, AU - Sławek,Sonja, AU - Jaromin,Marcin, AU - Hus,Konrad K, AU - Buczkowicz,Justyna, AU - Łysiak,Dawid, AU - Petrílla,Vladimir, AU - Petrillova,Monika, AU - Legáth,Jaroslav, Y1 - 2020/03/08/ PY - 2020/02/06/received PY - 2020/03/05/revised PY - 2020/03/06/accepted PY - 2020/3/19/entrez PY - 2020/3/19/pubmed PY - 2020/3/19/medline KW - Agkistrodon contortrix KW - Naja ashei KW - protein concentration KW - snake venom JF - Animals : an open access journal from MDPI JO - Animals (Basel) VL - 10 IS - 3 N2 - Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms. SN - 2076-2615 UR - https://www.unboundmedicine.com/medline/citation/32182656/Comparison_of_Methods_for_Measuring_Protein_Concentration_in_Venom_Samples L2 - https://www.mdpi.com/resolver?pii=ani10030448 DB - PRIME DP - Unbound Medicine ER -
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