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Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction.
Molecules. 2020 Mar 15; 25(6)M

Abstract

Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5-60 min), extraction temperature (50-200 °C) and collector vessel temperature (25-200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector's vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly.

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Authors+Show Affiliations

Nuapia Y
Molecular Sciences Institute, School of Chemistry, University of Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa.
Tutu H
Molecular Sciences Institute, School of Chemistry, University of Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa.
Chimuka L
Molecular Sciences Institute, School of Chemistry, University of Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa.
Cukrowska E
Molecular Sciences Institute, School of Chemistry, University of Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa.

MeSH

CannabidiolCannabinoidsCannabinolCannabisChromatography, High Pressure LiquidDronabinolHallucinogensHot TemperaturePlant ExtractsSeedsWater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32183432

Citation

Nuapia, Yannick, et al. "Selective Extraction of Cannabinoid Compounds From Cannabis Seed Using Pressurized Hot Water Extraction." Molecules (Basel, Switzerland), vol. 25, no. 6, 2020.
Nuapia Y, Tutu H, Chimuka L, et al. Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction. Molecules. 2020;25(6).
Nuapia, Y., Tutu, H., Chimuka, L., & Cukrowska, E. (2020). Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction. Molecules (Basel, Switzerland), 25(6). https://doi.org/10.3390/molecules25061335
Nuapia Y, et al. Selective Extraction of Cannabinoid Compounds From Cannabis Seed Using Pressurized Hot Water Extraction. Molecules. 2020 Mar 15;25(6) PubMed PMID: 32183432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction. AU - Nuapia,Yannick, AU - Tutu,Hlanganani, AU - Chimuka,Luke, AU - Cukrowska,Ewa, Y1 - 2020/03/15/ PY - 2020/02/17/received PY - 2020/03/03/revised PY - 2020/03/04/accepted PY - 2020/3/19/entrez PY - 2020/3/19/pubmed PY - 2020/12/15/medline KW - cannabinoid compounds KW - pressurized hot water extraction KW - response surface methodology JF - Molecules (Basel, Switzerland) JO - Molecules VL - 25 IS - 6 N2 - Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5-60 min), extraction temperature (50-200 °C) and collector vessel temperature (25-200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector's vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/32183432/Selective_Extraction_of_Cannabinoid_Compounds_from_Cannabis_Seed_Using_Pressurized_Hot_Water_Extraction_ DB - PRIME DP - Unbound Medicine ER -