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Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii.
Front Microbiol. 2020; 11:308.FM

Abstract

Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed.

Authors+Show Affiliations

Laboratorio de Ecología Molecular, Instituto de Ecología y Biodiversidad, Facultad de Ciencias, Universidad de Chile, Santiago, Chile. Laboratorio de Ecología Microbiana, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.Laboratorio de Ecología Molecular, Instituto de Ecología y Biodiversidad, Facultad de Ciencias, Universidad de Chile, Santiago, Chile. Aix Marseille University, Univ Toulon, CNRS, IRD, Mediterranean Institute of Oceanography (MIO) UM 110, Marseille, France.Laboratorio de Ecología Molecular, Instituto de Ecología y Biodiversidad, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.Laboratorio de Ecología Microbiana, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32184772

Citation

Schwob, Guillaume, et al. "Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus Agassizii." Frontiers in Microbiology, vol. 11, 2020, p. 308.
Schwob G, Cabrol L, Poulin E, et al. Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii. Front Microbiol. 2020;11:308.
Schwob, G., Cabrol, L., Poulin, E., & Orlando, J. (2020). Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii. Frontiers in Microbiology, 11, 308. https://doi.org/10.3389/fmicb.2020.00308
Schwob G, et al. Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus Agassizii. Front Microbiol. 2020;11:308. PubMed PMID: 32184772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii. AU - Schwob,Guillaume, AU - Cabrol,Léa, AU - Poulin,Elie, AU - Orlando,Julieta, Y1 - 2020/02/28/ PY - 2019/11/26/received PY - 2020/02/11/accepted PY - 2020/3/19/entrez PY - 2020/3/19/pubmed PY - 2020/3/19/medline KW - Abatus agassizii KW - Antarctica KW - West Antarctic Peninsula KW - core-microbiota KW - deposit-feeder KW - gut microbiota KW - heart sea urchin KW - keystone SP - 308 EP - 308 JF - Frontiers in microbiology JO - Front Microbiol VL - 11 N2 - Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/32184772/Characterization_of_the_Gut_Microbiota_of_the_Antarctic_Heart_Urchin_(Spatangoida)_Abatus_agassizii L2 - https://doi.org/10.3389/fmicb.2020.00308 DB - PRIME DP - Unbound Medicine ER -
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