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Multiparametric autoantibody profiling of patients with systemic sclerosis in Greece.
Mediterr J Rheumatol. 2018 Sep; 29(3):120-126.MJ

Abstract

Background

Systemic sclerosis (SSc) is an autoimmune rheumatic disease characterized by a wide range of disease-specific and disease-related autoantibodies (autoAbs). Profile assays have been developed and are currently in use to meet the demand for better characterization of all autoAbs found in SSc patients.

Aim

To assess the clinical relevance of SSc-related autoantibodies in 158 patients with SSc, all from Central Greece, taking advantage of a multiparametric SSc autoantibody line immunoassay.

Material and methods

158 consecutive patients with SSc (137 females, mean age 53.2 ± 10 years; 63 patients with dcSSc and 95 with lcSSc) from central Greece were included in the study. Eighteen patients with morphea were also included. Serum samples were analyzed by a profile SSc nucleoli line assay (Euroimmun) to detect Abs against 13 autoantigens: Scl-70, Centromere (A, B), RNA polymerase III (subunits 11 & 155), fibrillarin, NOR90, Th/To, PM/Scl 100, PM/Scl75, Ku, PDGFR and Ro52. Antinuclear autoAbs (ANAs) were detected by indirect immunofluorescence.

Results

ANAs were detected in 97.5% of SSc patients. Reactivities to specific autoantigens were as follows: Topo I, 40.5%; CENP, 32.9%; Ro52, 21.5%; RP11, 8.9%; RP155, 13.3%; NOR 90, 4.4%; Ku 3.8%; PM-Scl75, 3.2%; PM-Scl100, 1.3%; Th/To, 1.3%; Fibrillarin, 1.3%; PDGFR 0%; Ro52 21.5%. Twenty-one of SSc did not have any of the main autoAbs, namely anti-Topo I, anti-CENP, anti-RNA pol III Abs.

Conclusions

Multiparametric autoAb test provides positive SSc-associated autoAb reactivities in SSc patients negative for the three main autoAbs and this may prove of significance in early disease diagnosis.

Authors+Show Affiliations

Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Institute of Immunology affiliated to Euroimmun AG, Lübeck, Germany.Institute of Immunology affiliated to Euroimmun AG, Lübeck, Germany.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32185313

Citation

Liaskos, Christos, et al. "Multiparametric Autoantibody Profiling of Patients With Systemic Sclerosis in Greece." Mediterranean Journal of Rheumatology, vol. 29, no. 3, 2018, pp. 120-126.
Liaskos C, Marou E, Simopoulou T, et al. Multiparametric autoantibody profiling of patients with systemic sclerosis in Greece. Mediterr J Rheumatol. 2018;29(3):120-126.
Liaskos, C., Marou, E., Simopoulou, T., Gkoutzourelas, A., Barmakoudi, M., Efthymiou, G., Scheper, T., Meyer, W., Katsiari, C. G., Bogdanos, D. P., & Sakkas, L. I. (2018). Multiparametric autoantibody profiling of patients with systemic sclerosis in Greece. Mediterranean Journal of Rheumatology, 29(3), 120-126. https://doi.org/10.31138/mjr.29.3.120
Liaskos C, et al. Multiparametric Autoantibody Profiling of Patients With Systemic Sclerosis in Greece. Mediterr J Rheumatol. 2018;29(3):120-126. PubMed PMID: 32185313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiparametric autoantibody profiling of patients with systemic sclerosis in Greece. AU - Liaskos,Christos, AU - Marou,Emmanouela, AU - Simopoulou,Theodora, AU - Gkoutzourelas,Athanasios, AU - Barmakoudi,Maria, AU - Efthymiou,George, AU - Scheper,Thomas, AU - Meyer,Wolfgang, AU - Katsiari,Christina G, AU - Bogdanos,Dimitrios P, AU - Sakkas,Lazaros I, Y1 - 2018/09/27/ PY - 2018/06/11/received PY - 2018/09/13/revised PY - 2018/09/23/accepted PY - 2020/3/19/entrez PY - 2018/9/27/pubmed PY - 2018/9/27/medline KW - autoantibodies KW - autoimmune disease KW - diagnostics KW - scleroderma SP - 120 EP - 126 JF - Mediterranean journal of rheumatology JO - Mediterr J Rheumatol VL - 29 IS - 3 N2 - Background: Systemic sclerosis (SSc) is an autoimmune rheumatic disease characterized by a wide range of disease-specific and disease-related autoantibodies (autoAbs). Profile assays have been developed and are currently in use to meet the demand for better characterization of all autoAbs found in SSc patients. Aim: To assess the clinical relevance of SSc-related autoantibodies in 158 patients with SSc, all from Central Greece, taking advantage of a multiparametric SSc autoantibody line immunoassay. Material and methods: 158 consecutive patients with SSc (137 females, mean age 53.2 ± 10 years; 63 patients with dcSSc and 95 with lcSSc) from central Greece were included in the study. Eighteen patients with morphea were also included. Serum samples were analyzed by a profile SSc nucleoli line assay (Euroimmun) to detect Abs against 13 autoantigens: Scl-70, Centromere (A, B), RNA polymerase III (subunits 11 & 155), fibrillarin, NOR90, Th/To, PM/Scl 100, PM/Scl75, Ku, PDGFR and Ro52. Antinuclear autoAbs (ANAs) were detected by indirect immunofluorescence. Results: ANAs were detected in 97.5% of SSc patients. Reactivities to specific autoantigens were as follows: Topo I, 40.5%; CENP, 32.9%; Ro52, 21.5%; RP11, 8.9%; RP155, 13.3%; NOR 90, 4.4%; Ku 3.8%; PM-Scl75, 3.2%; PM-Scl100, 1.3%; Th/To, 1.3%; Fibrillarin, 1.3%; PDGFR 0%; Ro52 21.5%. Twenty-one of SSc did not have any of the main autoAbs, namely anti-Topo I, anti-CENP, anti-RNA pol III Abs. Conclusions: Multiparametric autoAb test provides positive SSc-associated autoAb reactivities in SSc patients negative for the three main autoAbs and this may prove of significance in early disease diagnosis. SN - 2529-198X UR - https://www.unboundmedicine.com/medline/citation/32185313/Multiparametric_autoantibody_profiling_of_patients_with_systemic_sclerosis_in_Greece_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32185313/ DB - PRIME DP - Unbound Medicine ER -