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[Complete mitochondrial genomes of Paragonimus westermani in China and phylogenetic analysis of various geographical isolates].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Jan 02; 32(1):28-35.ZX

Abstract

OBJECTIVE

To compare the complete mitochondrial genome sequences of two phenotypes of Paragonimus westermani isolated from Fujian Province with different sizes of metacercariae, and perform a phylogenetic analysis of various geographical isolates of P. westermani from Asia, so as to identify the possible genetic characteristics associated with the P. westermani phenotypes.

METHODS

P. westermani metacercariae with different sizes (large metacercariae, 380-420 μm in diameter; small metacercariae, 320-340 μm) isolated from freshwater crabs were used to infect dogs, and the eggs and adult worms of P. westermani were collected from the dog stool samples and lung tissues. Then, the egg size and morphology were compared. In addition, genomic DNA was extracted from the adult worms of the two phenotypes of P. westermani and used for the PCR amplification to yield the complete mitochondrial genome sequence. Sequence structure and phylogenetic analyses were performed based on the complete mitochondrial genome of P. westermani.

RESULTS

Following infection with large and small P. westermani metacercariae, the adult worms recovered from the dog lung had a thick body, and had oral and ventral suckers. The ventral sucker was located slightly in front of the midline of the body, and testes, ovary and vitelline gland were seen in the adult worms. Following fixation, the adults appeared oval, with an approximately 1.7∶1 of the length-width ratio. The length and width of the eggs isolated from the fecal samples of dogs infected with large and small P. westermani metacercariae varied significantly, and the large metacercariae produced bigger eggs than the smaller metacercariae. Based on the morphological features of adults and eggs and the ITS2 sequences, both phenotypes were identified as P. westermani. The complete mitochondrial genome sequence analysis of adults showed almost consistent sequences in the protein-coding region of the mitochondrial genome of adult worms derived from large and small metacercariae, with a major variation seen in the former non-coding region. Sliding window analysis revealed the most polymorphic region within the ND4 gene across the mitochondrial genome from various geographical isolates of P. westermani, and phylogenetic analysis showed that both phenotypes were clustered into the Chinese branch of P. westermani, which was close to the Japanese branch and distinct from the South/Southeast Asian branch.

CONCLUSIONS

The genetic distance between the phenotypes of P. westermani isolated from Fujian Province is near at a mitochondrial genome level, with no remarkable genetic differentiation seen; however, the mutation and structural changes in the non-coding region may result in the phenotypic variations. In addition, there is a distinct variation of the evolutionary rate in the mitochondrial coding genes, suggesting the selection of appropriate molecular markers during the phylogenic researches.

Authors+Show Affiliations

Department of Parasitology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.Department of Parasitology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.Fujian Provincial Center for Disease Control and Prevention, China.Department of Parasitology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.Department of Parasitology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32185925

Citation

Gu, M J., et al. "[Complete Mitochondrial Genomes of Paragonimus Westermani in China and Phylogenetic Analysis of Various Geographical Isolates]." Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi = Chinese Journal of Schistosomiasis Control, vol. 32, no. 1, 2020, pp. 28-35.
Gu MJ, Huang WL, Li YS, et al. [Complete mitochondrial genomes of Paragonimus westermani in China and phylogenetic analysis of various geographical isolates]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020;32(1):28-35.
Gu, M. J., Huang, W. L., Li, Y. S., Dong, H. F., & Zhao, Q. P. (2020). [Complete mitochondrial genomes of Paragonimus westermani in China and phylogenetic analysis of various geographical isolates]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi = Chinese Journal of Schistosomiasis Control, 32(1), 28-35. https://doi.org/10.16250/j.32.1374.2019238
Gu MJ, et al. [Complete Mitochondrial Genomes of Paragonimus Westermani in China and Phylogenetic Analysis of Various Geographical Isolates]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Jan 2;32(1):28-35. PubMed PMID: 32185925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Complete mitochondrial genomes of Paragonimus westermani in China and phylogenetic analysis of various geographical isolates]. AU - Gu,M J, AU - Huang,W L, AU - Li,Y S, AU - Dong,H F, AU - Zhao,Q P, PY - 2020/3/19/entrez PY - 2020/3/19/pubmed PY - 2020/3/25/medline KW - Genetic differentiation KW - Mitochondrial genome KW - Paragonimus westermani KW - Phenotype KW - Phylogeny SP - 28 EP - 35 JF - Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control JO - Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi VL - 32 IS - 1 N2 - OBJECTIVE: To compare the complete mitochondrial genome sequences of two phenotypes of Paragonimus westermani isolated from Fujian Province with different sizes of metacercariae, and perform a phylogenetic analysis of various geographical isolates of P. westermani from Asia, so as to identify the possible genetic characteristics associated with the P. westermani phenotypes. METHODS: P. westermani metacercariae with different sizes (large metacercariae, 380-420 μm in diameter; small metacercariae, 320-340 μm) isolated from freshwater crabs were used to infect dogs, and the eggs and adult worms of P. westermani were collected from the dog stool samples and lung tissues. Then, the egg size and morphology were compared. In addition, genomic DNA was extracted from the adult worms of the two phenotypes of P. westermani and used for the PCR amplification to yield the complete mitochondrial genome sequence. Sequence structure and phylogenetic analyses were performed based on the complete mitochondrial genome of P. westermani. RESULTS: Following infection with large and small P. westermani metacercariae, the adult worms recovered from the dog lung had a thick body, and had oral and ventral suckers. The ventral sucker was located slightly in front of the midline of the body, and testes, ovary and vitelline gland were seen in the adult worms. Following fixation, the adults appeared oval, with an approximately 1.7∶1 of the length-width ratio. The length and width of the eggs isolated from the fecal samples of dogs infected with large and small P. westermani metacercariae varied significantly, and the large metacercariae produced bigger eggs than the smaller metacercariae. Based on the morphological features of adults and eggs and the ITS2 sequences, both phenotypes were identified as P. westermani. The complete mitochondrial genome sequence analysis of adults showed almost consistent sequences in the protein-coding region of the mitochondrial genome of adult worms derived from large and small metacercariae, with a major variation seen in the former non-coding region. Sliding window analysis revealed the most polymorphic region within the ND4 gene across the mitochondrial genome from various geographical isolates of P. westermani, and phylogenetic analysis showed that both phenotypes were clustered into the Chinese branch of P. westermani, which was close to the Japanese branch and distinct from the South/Southeast Asian branch. CONCLUSIONS: The genetic distance between the phenotypes of P. westermani isolated from Fujian Province is near at a mitochondrial genome level, with no remarkable genetic differentiation seen; however, the mutation and structural changes in the non-coding region may result in the phenotypic variations. In addition, there is a distinct variation of the evolutionary rate in the mitochondrial coding genes, suggesting the selection of appropriate molecular markers during the phylogenic researches. SN - 1005-6661 UR - https://www.unboundmedicine.com/medline/citation/32185925/[Complete_mitochondrial_genomes_of_Paragonimus_westermani_in_China_and_phylogenetic_analysis_of_various_geographical_isolates] DB - PRIME DP - Unbound Medicine ER -