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Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease.
J Tradit Chin Med. 2019 06; 39(3):356-363.JT

Abstract

OBJECTIVE

To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease, and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1R) in the colon, spinal dorsal horn and hypothalamus.

METHODS

The rats were randomly divided into the normal, model (CD), herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups. Damage in the colons was scored and observed by hematoxylin and eosin staining. DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry. The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay, and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.

RESULTS

In the colon, compared with the normal group, DA, D1R protein expressions and D1R mRNA expression were significantly higher in the model group, while decreased in the Mox group and the Mesa group. In the spinal dorsal horn and hypothalamus, compared with the normal group, the concentrations of DA and D1R, and the D1R mRNA expressions were significantly higher in the model group, and decreased in the Mox group and the Mesa group.

CONCLUSION

Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats, the underlying mechanism maybe relative with the regulation of DA and D1R in the colon, spinal dorsal horn and hypothalamus by moxibustion.

Authors+Show Affiliations

Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China.

Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.

Department of Acupuncture and Moxibustion, Huashan Hospital, Shanghai 200040, China.

Department of Acupuncture and Moxibustion and Tuina, Guanghua Hospital, Shanghai 200052, China.

MeSH

AnimalsCentral Nervous SystemColonCrohn DiseaseDopamineIntestinal MucosaMaleMesalamineMoxibustionRandom AllocationRatsReal-Time Polymerase Chain ReactionReceptors, Dopamine

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32186008

Citation

Lu, Yuan, et al. "Effect of Herb-partitioned Moxibustion On Dopamine Levels and Dopamine Receptor 1 Expression in the Colon and Central Nervous System in Rats With Crohn's Disease." Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan, vol. 39, no. 3, 2019, pp. 356-363.

Lu Y, Ding G, Zheng H, et al. Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease. J Tradit Chin Med. 2019;39(3):356-363.

Lu, Y., Ding, G., Zheng, H., Lü, T., Ma, Z., Wu, H., Weng, Z., Zhang, F., Wu, L., Liu, H., Xu, M., & Feng, H. (2019). Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease. Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan, 39(3), 356-363.

Lu Y, et al. Effect of Herb-partitioned Moxibustion On Dopamine Levels and Dopamine Receptor 1 Expression in the Colon and Central Nervous System in Rats With Crohn's Disease. J Tradit Chin Med. 2019;39(3):356-363. PubMed PMID: 32186008.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease. AU - Lu,Yuan, AU - Ding,Guanghong, AU - Zheng,Handan, AU - Lü,Tingting, AU - Ma,Zhe, AU - Wu,Huangan, AU - Weng,Zhijun, AU - Zhang,Fang, AU - Wu,Luyi, AU - Liu,Huirong, AU - Xu,Manwen, AU - Feng,Hui, PY - 2020/3/19/entrez PY - 2020/3/19/pubmed PY - 2020/7/24/medline KW - Crohn disease KW - Dopamine KW - Herb-partitioned moxibustion KW - Neuropeptides KW - Point CV6 (Qihai) KW - Point ST25 (Tianshu) KW - Receptors, dopamine SP - 356 EP - 363 JF - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JO - J Tradit Chin Med VL - 39 IS - 3 N2 - OBJECTIVE: To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease, and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1R) in the colon, spinal dorsal horn and hypothalamus. METHODS: The rats were randomly divided into the normal, model (CD), herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups. Damage in the colons was scored and observed by hematoxylin and eosin staining. DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry. The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay, and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction. RESULTS: In the colon, compared with the normal group, DA, D1R protein expressions and D1R mRNA expression were significantly higher in the model group, while decreased in the Mox group and the Mesa group. In the spinal dorsal horn and hypothalamus, compared with the normal group, the concentrations of DA and D1R, and the D1R mRNA expressions were significantly higher in the model group, and decreased in the Mox group and the Mesa group. CONCLUSION: Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats, the underlying mechanism maybe relative with the regulation of DA and D1R in the colon, spinal dorsal horn and hypothalamus by moxibustion. SN - 2589-451X UR - https://www.unboundmedicine.com/medline/citation/32186008/Effect_of_herb_partitioned_moxibustion_on_dopamine_levels_and_dopamine_receptor_1_expression_in_the_colon_and_central_nervous_system_in_rats_with_Crohn's_disease_ DB - PRIME DP - Unbound Medicine ER -