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Efficacy of herb-partitioned moxibustion at Qihai (CV 6) and bilateral Tianshu (ST 25) on colonic damage and the TLR4/NF-κB signaling pathway in rats with Crohn's disease.
J Tradit Chin Med. 2018 Apr; 38(2):218-226.JT

Abstract

OBJECTIVE

To observe the effect of stimulating Qihai (CV 6) and bilateral Tianshu (ST 25) with herb-partitioned moxibustion (HPM) in rats with Crohn's disease (CD), and to investigate the possible anti-inflammatory mechanism of HPM.

METHODS

Forty rats were randomly divided into four groups (n = 10 rats per group): normal control (NC), model control (MC), mesalamine (MES), and HPM. The CD rat model was established in the MC, MES, and HPM groups by administering a mixture of trinitrobenzenesulfonic acid and alcohol via enema. The HPM group received HPM on Qihai (CV 6) and bilateral Tianshu (ST 25), while the MES group received intragastric mesalamine. Colonic histomorphological scores, and serum concentrations of tumor necrosis factor ¦Á (TNF-¦Á) and interleukin 1¦Â (IL-1¦Â) were assessed to evaluate the effects of HPM on colonic reparation and anti-inflammation. The expressions of Toll-like receptor 4 (TLR-4), nuclear factor ¦ÊB inhibitor ¦Á (I¦ÊB-¦Á), I¦ÊB kinase ¦Á/¦Â (IKK¦Á/¦Â), and NF-¦ÊB p65 were further analyzed to investigate the regulatory effects of the interventions on the TLR4/NF-¦ÊB pathway.

RESULTS

CD rats showed inflammatory colonic damage and increased serum concentrations of TNF-¦Á and IL-1¦Â. The expressions of TLR4, IKK¦Á/¦Â, and NF-¦ÊB p65 in the colons of CD rats were significantly increased compared with the NC group, while the expression of I¦ÊB¦Á (a key negative regulator of NF-¦ÊB p65) was decreased. HPM significantly mitigated colonic damage and reduced the serum concentrations of TNF-α and IL-1¦Â. HPM downregulated the expressions of TLR4, IKK¦Á/¦Â, and NF-¦ÊB p65 in the colon, and upregulated the expression of I¦ÊB¦Á. The effects of HPM in CD rats were similar to those of mesalamine.

CONCLUSION

HPM alleviates colonic inflammation in CD rats. This may be achieved through regulation of TLR4, which induces NF-¦ÊB signal transduction.

Authors+Show Affiliations

Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Key Laboratory for Acupuncture-Moxibustion and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China.Department of Acupuncture and Moxibustion, Shanghai Huangpu Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200010, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32186061

Citation

Zhang, Dan, et al. "Efficacy of Herb-partitioned Moxibustion at Qihai (CV 6) and Bilateral Tianshu (ST 25) On Colonic Damage and the TLR4/NF-κB Signaling Pathway in Rats With Crohn's Disease." Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan, vol. 38, no. 2, 2018, pp. 218-226.
Zhang D, Ma X, Wu H, et al. Efficacy of herb-partitioned moxibustion at Qihai (CV 6) and bilateral Tianshu (ST 25) on colonic damage and the TLR4/NF-κB signaling pathway in rats with Crohn's disease. J Tradit Chin Med. 2018;38(2):218-226.
Zhang, D., Ma, X., Wu, H., Hong, J., Zhang, C., Wu, L., Liu, J., Zhu, Y., Yang, L., Wei, K., & Yan, H. (2018). Efficacy of herb-partitioned moxibustion at Qihai (CV 6) and bilateral Tianshu (ST 25) on colonic damage and the TLR4/NF-κB signaling pathway in rats with Crohn's disease. Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan, 38(2), 218-226.
Zhang D, et al. Efficacy of Herb-partitioned Moxibustion at Qihai (CV 6) and Bilateral Tianshu (ST 25) On Colonic Damage and the TLR4/NF-κB Signaling Pathway in Rats With Crohn's Disease. J Tradit Chin Med. 2018;38(2):218-226. PubMed PMID: 32186061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of herb-partitioned moxibustion at Qihai (CV 6) and bilateral Tianshu (ST 25) on colonic damage and the TLR4/NF-κB signaling pathway in rats with Crohn's disease. AU - Zhang,Dan, AU - Ma,Xiaopeng, AU - Wu,Huangan, AU - Hong,Jue, AU - Zhang,Cuihong, AU - Wu,Lingxiang, AU - Liu,Jie, AU - Zhu,Yi, AU - Yang,Ling, AU - Wei,Kai, AU - Yan,Huang, PY - 2020/3/19/entrez PY - 2018/4/1/pubmed PY - 2018/4/1/medline KW - Anti-inflammation KW - Crohn's disease KW - Moxibustion KW - Rats KW - TLR4/NF-¦ÊB pathway SP - 218 EP - 226 JF - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JO - J Tradit Chin Med VL - 38 IS - 2 N2 - OBJECTIVE: To observe the effect of stimulating Qihai (CV 6) and bilateral Tianshu (ST 25) with herb-partitioned moxibustion (HPM) in rats with Crohn's disease (CD), and to investigate the possible anti-inflammatory mechanism of HPM. METHODS: Forty rats were randomly divided into four groups (n = 10 rats per group): normal control (NC), model control (MC), mesalamine (MES), and HPM. The CD rat model was established in the MC, MES, and HPM groups by administering a mixture of trinitrobenzenesulfonic acid and alcohol via enema. The HPM group received HPM on Qihai (CV 6) and bilateral Tianshu (ST 25), while the MES group received intragastric mesalamine. Colonic histomorphological scores, and serum concentrations of tumor necrosis factor ¦Á (TNF-¦Á) and interleukin 1¦Â (IL-1¦Â) were assessed to evaluate the effects of HPM on colonic reparation and anti-inflammation. The expressions of Toll-like receptor 4 (TLR-4), nuclear factor ¦ÊB inhibitor ¦Á (I¦ÊB-¦Á), I¦ÊB kinase ¦Á/¦Â (IKK¦Á/¦Â), and NF-¦ÊB p65 were further analyzed to investigate the regulatory effects of the interventions on the TLR4/NF-¦ÊB pathway. RESULTS: CD rats showed inflammatory colonic damage and increased serum concentrations of TNF-¦Á and IL-1¦Â. The expressions of TLR4, IKK¦Á/¦Â, and NF-¦ÊB p65 in the colons of CD rats were significantly increased compared with the NC group, while the expression of I¦ÊB¦Á (a key negative regulator of NF-¦ÊB p65) was decreased. HPM significantly mitigated colonic damage and reduced the serum concentrations of TNF-α and IL-1¦Â. HPM downregulated the expressions of TLR4, IKK¦Á/¦Â, and NF-¦ÊB p65 in the colon, and upregulated the expression of I¦ÊB¦Á. The effects of HPM in CD rats were similar to those of mesalamine. CONCLUSION: HPM alleviates colonic inflammation in CD rats. This may be achieved through regulation of TLR4, which induces NF-¦ÊB signal transduction. SN - 2589-451X UR - https://www.unboundmedicine.com/medline/citation/32186061/Efficacy_of_herb_partitioned_moxibustion_at_Qihai__CV_6__and_bilateral_Tianshu__ST_25__on_colonic_damage_and_the_TLR4/NF_κB_signaling_pathway_in_rats_with_Crohn's_disease_ DB - PRIME DP - Unbound Medicine ER -
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