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Identification of suspected paragonimiasis-endemic foci using a questionnaire and detection of Paragonimus ova using the Ziehl-Neelsen technique in Zamboanga Region, the Philippines.
Pathog Glob Health. 2020 05; 114(3):127-135.PG

Abstract

Improving paragonimiasis surveillance, which is crucial for disease control, requires adopting new tools and techniques useful in mapping endemic areas. This study aimed to (1) develop a questionnaire to identify suspected paragonimiasis-endemic foci, (2) describe the epidemiology of paragonimiasis, and (3) evaluate Ziehl-Nielsen Staining technique (ZNS) in detecting Paragonimus ova. The questionnaire, which municipal health officers filled out, was based on proposed site inclusion criteria utilized in the integrated tuberculosis (TB)-paragonimiasis surveillance and control project. Newly deployed medical technologists in Zamboanga Region underwent training, which included laboratory diagnosis of paragonimiasis using preserved and fresh specimens and an integrated tuberculosis-paragonimiasis survey in nine selected barangays (villages). Paragonimiasis cases were found in seven out of the nine barangays identified by the questionnaire. Of the 373 patients, three (0.80%) were TB-positive, and 29 (7.77%) were paragonimiasis-positive. The highest paragonimiasis prevalence (27%) was found in Barangay Libato. Ziehl-Neelsen Staining technique (ZNS) correctly detected 8 out of the 29 samples positive (sensitivity - 27.59%; 95% CI: 12.73-47.24%) and all the 334 samples negative (specificity - 100%; 95% CI: 98.90-100%) for Paragonimus ova. The questionnaire may be improved by refining the inclusion criteria. In paragonimiasis-endemic areas, the ZNS and the NaOH concentration technique may be used for detecting Paragonimus ova. Modifying the ZNS, for instance by including a concentration step, may improve its sensitivity. The model for the integrated capacity building of health workers and surveillance and research demonstrated in this project may contribute to improving surveillance and control of paragonimiasis and other neglected tropical diseases.

Authors+Show Affiliations

Department of Parasitology, College of Public Health and Neglected Tropical Diseases Study Group, National Institutes of Health, University of the Philippines Manila, Manila, Philippines. Kirby Institute, Faculty of Medicine, University of New South Wales, Sydney, Australia.Institute of Clinical Epidemiology, National Institutes of Health, University of the Philippines Manila, Manila, Philippines.Department of Health Zamboanga Peninsula Center for Health Development, DOH ZP CHD, Zamboanga City, Philippines.Department of Parasitology, College of Public Health and Neglected Tropical Diseases Study Group, National Institutes of Health, University of the Philippines Manila, Manila, Philippines.Department of Physiology, College of Medicine, University of the Philippines Manila, Manila, Philippines.Department of Parasitology, College of Public Health and Neglected Tropical Diseases Study Group, National Institutes of Health, University of the Philippines Manila, Manila, Philippines.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32191613

Citation

Delos Trinos, John Paul Caesar, et al. "Identification of Suspected Paragonimiasis-endemic Foci Using a Questionnaire and Detection of Paragonimus Ova Using the Ziehl-Neelsen Technique in Zamboanga Region, the Philippines." Pathogens and Global Health, vol. 114, no. 3, 2020, pp. 127-135.
Delos Trinos JPC, Sison OT, Anino MRC, et al. Identification of suspected paragonimiasis-endemic foci using a questionnaire and detection of Paragonimus ova using the Ziehl-Neelsen technique in Zamboanga Region, the Philippines. Pathog Glob Health. 2020;114(3):127-135.
Delos Trinos, J. P. C., Sison, O. T., Anino, M. R. C., Lacuna, J. D. M., Jorge, M. C., & Belizario, V. Y. (2020). Identification of suspected paragonimiasis-endemic foci using a questionnaire and detection of Paragonimus ova using the Ziehl-Neelsen technique in Zamboanga Region, the Philippines. Pathogens and Global Health, 114(3), 127-135. https://doi.org/10.1080/20477724.2020.1741900
Delos Trinos JPC, et al. Identification of Suspected Paragonimiasis-endemic Foci Using a Questionnaire and Detection of Paragonimus Ova Using the Ziehl-Neelsen Technique in Zamboanga Region, the Philippines. Pathog Glob Health. 2020;114(3):127-135. PubMed PMID: 32191613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of suspected paragonimiasis-endemic foci using a questionnaire and detection of Paragonimus ova using the Ziehl-Neelsen technique in Zamboanga Region, the Philippines. AU - Delos Trinos,John Paul Caesar, AU - Sison,Olivia T, AU - Anino,Maria Reiza C, AU - Lacuna,Jana Denise M, AU - Jorge,Manuel C, AU - Belizario,Vicente Y, Y1 - 2020/03/19/ PY - 2021/03/19/pmc-release PY - 2020/3/20/pubmed PY - 2020/3/20/medline PY - 2020/3/20/entrez KW - NaOH concentration technique KW - Paragonimiasis KW - Philippines KW - Zamboanga Region KW - Ziehl–Neelsen Staining technique KW - pulmonary tuberculosis KW - questionnaire SP - 127 EP - 135 JF - Pathogens and global health JO - Pathog Glob Health VL - 114 IS - 3 N2 - Improving paragonimiasis surveillance, which is crucial for disease control, requires adopting new tools and techniques useful in mapping endemic areas. This study aimed to (1) develop a questionnaire to identify suspected paragonimiasis-endemic foci, (2) describe the epidemiology of paragonimiasis, and (3) evaluate Ziehl-Nielsen Staining technique (ZNS) in detecting Paragonimus ova. The questionnaire, which municipal health officers filled out, was based on proposed site inclusion criteria utilized in the integrated tuberculosis (TB)-paragonimiasis surveillance and control project. Newly deployed medical technologists in Zamboanga Region underwent training, which included laboratory diagnosis of paragonimiasis using preserved and fresh specimens and an integrated tuberculosis-paragonimiasis survey in nine selected barangays (villages). Paragonimiasis cases were found in seven out of the nine barangays identified by the questionnaire. Of the 373 patients, three (0.80%) were TB-positive, and 29 (7.77%) were paragonimiasis-positive. The highest paragonimiasis prevalence (27%) was found in Barangay Libato. Ziehl-Neelsen Staining technique (ZNS) correctly detected 8 out of the 29 samples positive (sensitivity - 27.59%; 95% CI: 12.73-47.24%) and all the 334 samples negative (specificity - 100%; 95% CI: 98.90-100%) for Paragonimus ova. The questionnaire may be improved by refining the inclusion criteria. In paragonimiasis-endemic areas, the ZNS and the NaOH concentration technique may be used for detecting Paragonimus ova. Modifying the ZNS, for instance by including a concentration step, may improve its sensitivity. The model for the integrated capacity building of health workers and surveillance and research demonstrated in this project may contribute to improving surveillance and control of paragonimiasis and other neglected tropical diseases. SN - 2047-7732 UR - https://www.unboundmedicine.com/medline/citation/32191613/Identification_of_suspected_paragonimiasis-endemic_foci_using_a_questionnaire_and_detection_of_Paragonimus_ova_using_the_Ziehl-Neelsen_technique_in_Zamboanga_Region,_the_Philippines L2 - http://www.tandfonline.com/doi/full/10.1080/20477724.2020.1741900 DB - PRIME DP - Unbound Medicine ER -
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