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Development and molecular cytogenetic characterization of Thinopyrum bessarabicum introgression lines in hexaploid and tetraploid wheats.
Theor Appl Genet. 2020 Jul; 133(7):2117-2130.TA

Abstract

KEY MESSAGE

A variety of Thinopyrum bessarabicum introgressions in both hexaploid and tetraploid wheats were generated and characterized by molecular cytogenetic analysis. Six wheat-J genome recombinants were identified with ND-FISH and GISH. Diploid wheatgrass, Thinopyrum bessarabicum (2n = 2x = 14, EbEb or JbJb or JJ), is a well-known alien source of salinity tolerance and disease resistance for wheat improvement. The true genetic potential and effect of such introgressions into wheat can be best studied in chromosomal addition or substitution lines. Here, we report the generation and characterization of various categories of Th. bessarabicum derivatives in both hexaploid and tetraploid cultivated wheats. Sequential non-denaturing fluorescence in situ hybridization (ND-FISH) and genomic in situ hybridization (GISH) are robust techniques to visualize the size of alien introgressions and breakpoints. We identified a complete set of monosomic addition lines into both bread wheat and durum wheat, except for 7J in durum wheat, by sequential ND-FISH and GISH. We also characterized alien derivatives belonging to various classes including mono-telosomic additions, disomic additions, monosomic substitutions, double monosomic substitutions, monosomic substitution-monosomic additions, double monosomic additions, and multiple monosomic additions into both bread and durum wheats. In addition, various wheat-Th. bessarabicum recombinant chromosomes were also detected in six alien derivatives. These wheat-Th. bessarabicum derivatives will provide useful cytogenetic resources for improvement of both hexaploid and tetraploid wheats.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Singh AK
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Zhang P
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia. peng.zhang@sydney.edu.au.
Dong C
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Li J
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, China.
Singh S
Bihar Agricultural University, Sabour, Bihar, 813210, India.
Trethowan RM
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Sharp PJ
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia. peter.sharp@sydney.edu.au.

MeSH

Chromosome MappingChromosomes, PlantCrosses, GeneticCytogeneticsDNA, PlantDisease ResistanceGenome, PlantGenotypeIn Situ Hybridization, FluorescenceKaryotypingMitosisPloidiesPoaceaeTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32198597

Citation

Singh, Amit K., et al. "Development and Molecular Cytogenetic Characterization of Thinopyrum Bessarabicum Introgression Lines in Hexaploid and Tetraploid Wheats." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 7, 2020, pp. 2117-2130.
Singh AK, Zhang P, Dong C, et al. Development and molecular cytogenetic characterization of Thinopyrum bessarabicum introgression lines in hexaploid and tetraploid wheats. Theor Appl Genet. 2020;133(7):2117-2130.
Singh, A. K., Zhang, P., Dong, C., Li, J., Singh, S., Trethowan, R. M., & Sharp, P. J. (2020). Development and molecular cytogenetic characterization of Thinopyrum bessarabicum introgression lines in hexaploid and tetraploid wheats. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(7), 2117-2130. https://doi.org/10.1007/s00122-020-03581-5
Singh AK, et al. Development and Molecular Cytogenetic Characterization of Thinopyrum Bessarabicum Introgression Lines in Hexaploid and Tetraploid Wheats. Theor Appl Genet. 2020;133(7):2117-2130. PubMed PMID: 32198597.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and molecular cytogenetic characterization of Thinopyrum bessarabicum introgression lines in hexaploid and tetraploid wheats. AU - Singh,Amit K, AU - Zhang,Peng, AU - Dong,Chongmei, AU - Li,Jianbo, AU - Singh,Smriti, AU - Trethowan,Richard M, AU - Sharp,Peter J, Y1 - 2020/03/20/ PY - 2019/10/01/received PY - 2020/03/12/accepted PY - 2020/3/22/pubmed PY - 2021/2/18/medline PY - 2020/3/22/entrez SP - 2117 EP - 2130 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 7 N2 - KEY MESSAGE: A variety of Thinopyrum bessarabicum introgressions in both hexaploid and tetraploid wheats were generated and characterized by molecular cytogenetic analysis. Six wheat-J genome recombinants were identified with ND-FISH and GISH. Diploid wheatgrass, Thinopyrum bessarabicum (2n = 2x = 14, EbEb or JbJb or JJ), is a well-known alien source of salinity tolerance and disease resistance for wheat improvement. The true genetic potential and effect of such introgressions into wheat can be best studied in chromosomal addition or substitution lines. Here, we report the generation and characterization of various categories of Th. bessarabicum derivatives in both hexaploid and tetraploid cultivated wheats. Sequential non-denaturing fluorescence in situ hybridization (ND-FISH) and genomic in situ hybridization (GISH) are robust techniques to visualize the size of alien introgressions and breakpoints. We identified a complete set of monosomic addition lines into both bread wheat and durum wheat, except for 7J in durum wheat, by sequential ND-FISH and GISH. We also characterized alien derivatives belonging to various classes including mono-telosomic additions, disomic additions, monosomic substitutions, double monosomic substitutions, monosomic substitution-monosomic additions, double monosomic additions, and multiple monosomic additions into both bread and durum wheats. In addition, various wheat-Th. bessarabicum recombinant chromosomes were also detected in six alien derivatives. These wheat-Th. bessarabicum derivatives will provide useful cytogenetic resources for improvement of both hexaploid and tetraploid wheats. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/32198597/Development_and_molecular_cytogenetic_characterization_of_Thinopyrum_bessarabicum_introgression_lines_in_hexaploid_and_tetraploid_wheats_ DB - PRIME DP - Unbound Medicine ER -