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Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses.
Biochem Biophys Res Commun. 2020 05 21; 526(1):135-140.BB

Abstract

The new coronavirus (SARS-CoV-2) outbreak from December 2019 in Wuhan, Hubei, China, has been declared a global public health emergency. Angiotensin I converting enzyme 2 (ACE2), is the host receptor by SARS-CoV-2 to infect human cells. Although ACE2 is reported to be expressed in lung, liver, stomach, ileum, kidney and colon, its expressing levels are rather low, especially in the lung. SARS-CoV-2 may use co-receptors/auxiliary proteins as ACE2 partner to facilitate the virus entry. To identify the potential candidates, we explored the single cell gene expression atlas including 119 cell types of 13 human tissues and analyzed the single cell co-expression spectrum of 51 reported RNA virus receptors and 400 other membrane proteins. Consistent with other recent reports, we confirmed that ACE2 was mainly expressed in lung AT2, liver cholangiocyte, colon colonocytes, esophagus keratinocytes, ileum ECs, rectum ECs, stomach epithelial cells, and kidney proximal tubules. Intriguingly, we found that the candidate co-receptors, manifesting the most similar expression patterns with ACE2 across 13 human tissues, are all peptidases, including ANPEP, DPP4 and ENPEP. Among them, ANPEP and DPP4 are the known receptors for human CoVs, suggesting ENPEP as another potential receptor for human CoVs. We also conducted "CellPhoneDB" analysis to understand the cell crosstalk between CoV-targets and their surrounding cells across different tissues. We found that macrophages frequently communicate with the CoVs targets through chemokine and phagocytosis signaling, highlighting the importance of tissue macrophages in immune defense and immune pathogenesis.

Authors+Show Affiliations

Institute of Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong Province, 518100, China.Institute of Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong Province, 518100, China.Shanghai Public Health Clinical Center and Institute of Biomedical Sciences, Fudan University, Shanghai, China. Electronic address: zhangshuye@shphc.org.cn.Institute of Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong Province, 518100, China; The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen, Guangdong Province, 518112, China. Electronic address: zhangzheng1975@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32199615

Citation

Qi, Furong, et al. "Single Cell RNA Sequencing of 13 Human Tissues Identify Cell Types and Receptors of Human Coronaviruses." Biochemical and Biophysical Research Communications, vol. 526, no. 1, 2020, pp. 135-140.
Qi F, Qian S, Zhang S, et al. Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses. Biochem Biophys Res Commun. 2020;526(1):135-140.
Qi, F., Qian, S., Zhang, S., & Zhang, Z. (2020). Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses. Biochemical and Biophysical Research Communications, 526(1), 135-140. https://doi.org/10.1016/j.bbrc.2020.03.044
Qi F, et al. Single Cell RNA Sequencing of 13 Human Tissues Identify Cell Types and Receptors of Human Coronaviruses. Biochem Biophys Res Commun. 2020 05 21;526(1):135-140. PubMed PMID: 32199615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses. AU - Qi,Furong, AU - Qian,Shen, AU - Zhang,Shuye, AU - Zhang,Zheng, Y1 - 2020/03/19/ PY - 2020/03/05/received PY - 2020/03/08/accepted PY - 2020/3/23/pubmed PY - 2020/4/29/medline PY - 2020/3/23/entrez KW - ACE2 KW - Co-receptor KW - Coronaviruses KW - Macrophage KW - SARS-CoV-2 KW - scRNA-seq SP - 135 EP - 140 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 526 IS - 1 N2 - The new coronavirus (SARS-CoV-2) outbreak from December 2019 in Wuhan, Hubei, China, has been declared a global public health emergency. Angiotensin I converting enzyme 2 (ACE2), is the host receptor by SARS-CoV-2 to infect human cells. Although ACE2 is reported to be expressed in lung, liver, stomach, ileum, kidney and colon, its expressing levels are rather low, especially in the lung. SARS-CoV-2 may use co-receptors/auxiliary proteins as ACE2 partner to facilitate the virus entry. To identify the potential candidates, we explored the single cell gene expression atlas including 119 cell types of 13 human tissues and analyzed the single cell co-expression spectrum of 51 reported RNA virus receptors and 400 other membrane proteins. Consistent with other recent reports, we confirmed that ACE2 was mainly expressed in lung AT2, liver cholangiocyte, colon colonocytes, esophagus keratinocytes, ileum ECs, rectum ECs, stomach epithelial cells, and kidney proximal tubules. Intriguingly, we found that the candidate co-receptors, manifesting the most similar expression patterns with ACE2 across 13 human tissues, are all peptidases, including ANPEP, DPP4 and ENPEP. Among them, ANPEP and DPP4 are the known receptors for human CoVs, suggesting ENPEP as another potential receptor for human CoVs. We also conducted "CellPhoneDB" analysis to understand the cell crosstalk between CoV-targets and their surrounding cells across different tissues. We found that macrophages frequently communicate with the CoVs targets through chemokine and phagocytosis signaling, highlighting the importance of tissue macrophages in immune defense and immune pathogenesis. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/32199615/Single_cell_RNA_sequencing_of_13_human_tissues_identify_cell_types_and_receptors_of_human_coronaviruses_ DB - PRIME DP - Unbound Medicine ER -