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[Effect of medicated thread moxibustion of Chinese Zhuang Minority medicine on colonic inflammatory impairment and Th17/IL-17F signaling in rats with ulcerative colitis].
Zhen Ci Yan Jiu. 2020 Mar 25; 45(3):188-93.ZC

Abstract

OBJECTIVE

To observe the effect of medicated thread moxibustion of Zhuang Minority medicine on helper T cell 17 (Th17)/ Interleukin-17F(IL-17F) signaling pathway in ulcerative colitis (UC) rats, so as to explore its mechanisms underlying improvement of UC.

METHODS

Forty male SD rats were randomly divided into normal control, model, medication and medicated thread moxibustion (MTM) groups, with 10 rats in each group. The UC model was induced by free drinking of 4% dextran sulfate sodium (DSS) for 10 d. After successful modeling, rats of the medication group were treated by gavage of salazosulfapyridine (SASP). Medicated thread moxibustion was applied to unilateral "Tianshu" (ST25) and "Qihai" (CV6) alternatively for rats of the MTM group, once daily for 14 d. The body mass, stool shape, and fecal occult test were recorded and conducted daily to perform disease activity index (DAI) score. H.E. staining was used to display pathological changes of the colon tissue. The Th17 cells and IL-17F and retinoic acid related orphan receptor γ t (ROR-γt) in the colon tissue were detected by flow cytometry, and enzyme-linked immunosorbent assay (ELISA), respectively, and the expression levels of RORγt and IL-17F mRNA in colon tissue were detected by quantitive real-time PCR.

RESULTS

After modeling, the DAI score, colonic Th17 percentage, RORγt and IL-17F contents, and RORγt and IL-17F mRNA expression were significantly increased in the model group in contrast to the normal control group (P<0.01, P<0.05). Following the intervention, all the aforementioned indexes were reversed in both medication and MTM groups (P<0.01, P<0.05). No significant differences were found between the medication and MTM groups in the levels of the above mentioned indexes (P>0.05, except RORγt and IL-17F mRNA expression). H.E. staining showed disappearance of goblet cells, infiltration of a large number of inflammatory cells, exfoliation of the epithelial tissue and edema of colonic mucosal in rats of the model group, which was relatively milder in both medication and MTM groups.

CONCLUSION

Medicated thread moxibustion of Zhuang Minority medicine can reduce the inflammatory damage of colon tissue in UC rats, which is associated with its effects in suppressing the expression of RORγt, production of Th17 cells, and secretion of pro-inflammatory factor IL-17F in colon tissue.

Authors+Show Affiliations

Department of Anorectal, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.College of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004.College of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004.Department of Anorectal, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.College of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004.Department of Anorectal, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.Department of Anorectal, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.Department of Anorectal, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32202709

Citation

Zhang, Lei Chang, et al. "[Effect of Medicated Thread Moxibustion of Chinese Zhuang Minority Medicine On Colonic Inflammatory Impairment and Th17/IL-17F Signaling in Rats With Ulcerative Colitis]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 3, 2020, pp. 188-93.
Zhang LC, Hu LX, Xiao AJ, et al. [Effect of medicated thread moxibustion of Chinese Zhuang Minority medicine on colonic inflammatory impairment and Th17/IL-17F signaling in rats with ulcerative colitis]. Zhen Ci Yan Jiu. 2020;45(3):188-93.
Zhang, L. C., Hu, L. X., Xiao, A. J., Wang, X., Pei, X. Y., Ma, X. F., Ge, W., & Xiao, H. R. (2020). [Effect of medicated thread moxibustion of Chinese Zhuang Minority medicine on colonic inflammatory impairment and Th17/IL-17F signaling in rats with ulcerative colitis]. Zhen Ci Yan Jiu = Acupuncture Research, 45(3), 188-93. https://doi.org/10.13702/j.1000-0607.190051
Zhang LC, et al. [Effect of Medicated Thread Moxibustion of Chinese Zhuang Minority Medicine On Colonic Inflammatory Impairment and Th17/IL-17F Signaling in Rats With Ulcerative Colitis]. Zhen Ci Yan Jiu. 2020 Mar 25;45(3):188-93. PubMed PMID: 32202709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of medicated thread moxibustion of Chinese Zhuang Minority medicine on colonic inflammatory impairment and Th17/IL-17F signaling in rats with ulcerative colitis]. AU - Zhang,Lei Chang, AU - Hu,Li Xia, AU - Xiao,Ai Jiao, AU - Wang,Xue, AU - Pei,Xin Yi, AU - Ma,Xiao Fei, AU - Ge,Wei, AU - Xiao,Hui Rong, PY - 2020/3/24/entrez PY - 2020/3/24/pubmed PY - 2020/3/24/medline KW - Disease activity index KW - Helper T cell 17 KW - Interleukin­17Fa KW - Medicated thread moxibustion of Zhuang Minority medicine KW - Ulcerative colitis SP - 188 EP - 93 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 3 N2 - OBJECTIVE: To observe the effect of medicated thread moxibustion of Zhuang Minority medicine on helper T cell 17 (Th17)/ Interleukin-17F(IL-17F) signaling pathway in ulcerative colitis (UC) rats, so as to explore its mechanisms underlying improvement of UC. METHODS: Forty male SD rats were randomly divided into normal control, model, medication and medicated thread moxibustion (MTM) groups, with 10 rats in each group. The UC model was induced by free drinking of 4% dextran sulfate sodium (DSS) for 10 d. After successful modeling, rats of the medication group were treated by gavage of salazosulfapyridine (SASP). Medicated thread moxibustion was applied to unilateral "Tianshu" (ST25) and "Qihai" (CV6) alternatively for rats of the MTM group, once daily for 14 d. The body mass, stool shape, and fecal occult test were recorded and conducted daily to perform disease activity index (DAI) score. H.E. staining was used to display pathological changes of the colon tissue. The Th17 cells and IL-17F and retinoic acid related orphan receptor γ t (ROR-γt) in the colon tissue were detected by flow cytometry, and enzyme-linked immunosorbent assay (ELISA), respectively, and the expression levels of RORγt and IL-17F mRNA in colon tissue were detected by quantitive real-time PCR. RESULTS: After modeling, the DAI score, colonic Th17 percentage, RORγt and IL-17F contents, and RORγt and IL-17F mRNA expression were significantly increased in the model group in contrast to the normal control group (P<0.01, P<0.05). Following the intervention, all the aforementioned indexes were reversed in both medication and MTM groups (P<0.01, P<0.05). No significant differences were found between the medication and MTM groups in the levels of the above mentioned indexes (P>0.05, except RORγt and IL-17F mRNA expression). H.E. staining showed disappearance of goblet cells, infiltration of a large number of inflammatory cells, exfoliation of the epithelial tissue and edema of colonic mucosal in rats of the model group, which was relatively milder in both medication and MTM groups. CONCLUSION: Medicated thread moxibustion of Zhuang Minority medicine can reduce the inflammatory damage of colon tissue in UC rats, which is associated with its effects in suppressing the expression of RORγt, production of Th17 cells, and secretion of pro-inflammatory factor IL-17F in colon tissue. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32202709/[Effect_of_medicated_thread_moxibustion_of_Chinese_Zhuang_Minority_medicine_on_colonic_inflammatory_impairment_and_Th17/IL_17F_signaling_in_rats_with_ulcerative_colitis]_ DB - PRIME DP - Unbound Medicine ER -