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Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats.
Mater Sci Eng C Mater Biol Appl. 2020 May; 110:110709.MS

Abstract

The present study presents a green, cost efficient and easy synthesis method of silver nanoparticles (AgNPs) using an aqueous extract of Cornus sanguinea L. fruits (CS). The phytosynthesized silver nanoparticles were characterized using various analytical techniques such as UV-Vis absorption spectroscopy, which confirmed the formation of AgNPs and FTIR spectroscopy, in order to certify the role of the biomolecules present in the fruit extract as reducing and capping agents of the AgNPs. The UV-Vis absorption spectrum showed a broad band at 407 nm characteristic for colloidal silver. Transmission electron microscopy was conducted to investigate the shape and size of the silver nanoparticles, revealing a spherical shape with an average particle size of 18 nm. The antioxidant and anti-inflammatory activities of the fruit extract and green synthesized silver nanoparticles were assessed in vivo on experimental inflammation. The obtained results showed that CS and AgNPs reduced oxidative stress in parallel with increasing of antioxidant defense and diminished the COX-2 expressions. CS extract had a dual effect on NFkB activation depending on the time of testing while AgNPs increased NFkB phosphorylation at 48 h. These results suggested that both AgNPs and CS extract exhibited antioxidant and anti-inflammatory activities but with a different dynamics of action.

Authors+Show Affiliations

Research Center for Advanced Chemical Analysis, Instrumentation and Chemometrics (ANALYTICA), Faculty of Chemistry and Chemical Engineering, "Babeş-Bolyai" University, 11 Arany Janos Street, Cluj-Napoca 400028, Romania.Research Center for Advanced Chemical Analysis, Instrumentation and Chemometrics (ANALYTICA), Faculty of Chemistry and Chemical Engineering, "Babeş-Bolyai" University, 11 Arany Janos Street, Cluj-Napoca 400028, Romania. Electronic address: bianca@chem.ubbcluj.ro.Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca 400006, Romania.Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca 400006, Romania.Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Street, Cluj-Napoca 400372, Romania; Department of Biomedical Sciences, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre, Saint Kitts and Nevis.Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca 400006, Romania.Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca 400006, Romania. Electronic address: gabriela.filip@umfcluj.ro.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32204021

Citation

David, Luminita, et al. "Modulatory Effects of Cornus Sanguinea L. Mediated Green Synthesized Silver Nanoparticles On Oxidative Stress, COX-2/NOS2 and NFkB/pNFkB Expressions in Experimental Inflammation in Wistar Rats." Materials Science & Engineering. C, Materials for Biological Applications, vol. 110, 2020, p. 110709.
David L, Moldovan B, Baldea I, et al. Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats. Mater Sci Eng C Mater Biol Appl. 2020;110:110709.
David, L., Moldovan, B., Baldea, I., Olteanu, D., Bolfa, P., Clichici, S., & Filip, G. A. (2020). Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats. Materials Science & Engineering. C, Materials for Biological Applications, 110, 110709. https://doi.org/10.1016/j.msec.2020.110709
David L, et al. Modulatory Effects of Cornus Sanguinea L. Mediated Green Synthesized Silver Nanoparticles On Oxidative Stress, COX-2/NOS2 and NFkB/pNFkB Expressions in Experimental Inflammation in Wistar Rats. Mater Sci Eng C Mater Biol Appl. 2020;110:110709. PubMed PMID: 32204021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats. AU - David,Luminita, AU - Moldovan,Bianca, AU - Baldea,Ioana, AU - Olteanu,Diana, AU - Bolfa,Pompei, AU - Clichici,Simona, AU - Filip,Gabriela Adriana, Y1 - 2020/01/30/ PY - 2019/11/14/received PY - 2020/01/27/revised PY - 2020/01/29/accepted PY - 2020/3/25/entrez PY - 2020/3/25/pubmed PY - 2020/12/17/medline KW - Anti-inflammatory activity KW - Antioxidant capacity KW - Cornus sanguinea KW - Silver nanoparticles SP - 110709 EP - 110709 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 110 N2 - The present study presents a green, cost efficient and easy synthesis method of silver nanoparticles (AgNPs) using an aqueous extract of Cornus sanguinea L. fruits (CS). The phytosynthesized silver nanoparticles were characterized using various analytical techniques such as UV-Vis absorption spectroscopy, which confirmed the formation of AgNPs and FTIR spectroscopy, in order to certify the role of the biomolecules present in the fruit extract as reducing and capping agents of the AgNPs. The UV-Vis absorption spectrum showed a broad band at 407 nm characteristic for colloidal silver. Transmission electron microscopy was conducted to investigate the shape and size of the silver nanoparticles, revealing a spherical shape with an average particle size of 18 nm. The antioxidant and anti-inflammatory activities of the fruit extract and green synthesized silver nanoparticles were assessed in vivo on experimental inflammation. The obtained results showed that CS and AgNPs reduced oxidative stress in parallel with increasing of antioxidant defense and diminished the COX-2 expressions. CS extract had a dual effect on NFkB activation depending on the time of testing while AgNPs increased NFkB phosphorylation at 48 h. These results suggested that both AgNPs and CS extract exhibited antioxidant and anti-inflammatory activities but with a different dynamics of action. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/32204021/Modulatory_effects_of_Cornus_sanguinea_L._mediated_green_synthesized_silver_nanoparticles_on_oxidative_stress,_COX-2/NOS2_and_NFkB/pNFkB_expressions_in_experimental_inflammation_in_Wistar_rats. L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(19)34258-4 DB - PRIME DP - Unbound Medicine ER -