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Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo verbana.
Front Immunol. 2020; 11:370.FI

Abstract

The innate immune response represents a first-line defense against pathogen infection that has been widely conserved throughout evolution. Using the invertebrate Hirudo verbana (Annelida, Hirudinea) as an experimental model, we show here that the RNASET2 ribonuclease is directly involved in the immune response against Gram-positive bacteria. Injection of lipoteichoic acid (LTA), a key component of Gram-positive bacteria cell wall, into the leech body wall induced a massive migration of granulocytes and macrophages expressing TLR2 (the key receptor involved in the response to Gram-positive bacteria) toward the challenged/inoculated area. We hypothesized that the endogenous leech RNASET2 protein (HvRNASET2) might be involved in the antimicrobial response, as already described for other vertebrate ribonucleases, such as RNase3 and RNase7. In support of our hypothesis, HvRNASET2 was mainly localized in the granules of granulocytes, and its release in the extracellular matrix triggered the recruitment of macrophages toward the area stimulated with LTA. The activity of HvRNASET2 was also evaluated on Staphylococcus aureus living cells by means of light, transmission, and scanning electron microscopy analysis. HvRNASET2 injection triggered the formation of S. aureus clumps following a direct interaction with the bacterial cell wall, as demonstrated by immunogold assay. Taken together, our data support the notion that, during the early phase of leech immune response, granulocyte-released HvRNASET2 triggers bacterial clumps formation and, at the same time, actively recruits phagocytic macrophages in order to elicit a rapid and effective eradication of the infecting microorganisms from inoculated area.

Authors+Show Affiliations

Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Medicine and Surgery, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32210967

Citation

Baranzini, Nicolò, et al. "Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo Verbana." Frontiers in Immunology, vol. 11, 2020, p. 370.
Baranzini N, De Vito A, Orlandi VT, et al. Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo verbana. Front Immunol. 2020;11:370.
Baranzini, N., De Vito, A., Orlandi, V. T., Reguzzoni, M., Monti, L., de Eguileor, M., Rosini, E., Pollegioni, L., Tettamanti, G., Acquati, F., & Grimaldi, A. (2020). Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo verbana. Frontiers in Immunology, 11, 370. https://doi.org/10.3389/fimmu.2020.00370
Baranzini N, et al. Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo Verbana. Front Immunol. 2020;11:370. PubMed PMID: 32210967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo verbana. AU - Baranzini,Nicolò, AU - De Vito,Annarosaria, AU - Orlandi,Viviana Teresa, AU - Reguzzoni,Marcella, AU - Monti,Laura, AU - de Eguileor,Magda, AU - Rosini,Elena, AU - Pollegioni,Loredano, AU - Tettamanti,Gianluca, AU - Acquati,Francesco, AU - Grimaldi,Annalisa, Y1 - 2020/03/06/ PY - 2019/10/14/received PY - 2020/02/17/accepted PY - 2020/3/27/entrez PY - 2020/3/27/pubmed PY - 2020/3/27/medline KW - LTA KW - RNASET2 KW - antimicrobial activity KW - innate immunity KW - medicinal leech SP - 370 EP - 370 JF - Frontiers in immunology JO - Front Immunol VL - 11 N2 - The innate immune response represents a first-line defense against pathogen infection that has been widely conserved throughout evolution. Using the invertebrate Hirudo verbana (Annelida, Hirudinea) as an experimental model, we show here that the RNASET2 ribonuclease is directly involved in the immune response against Gram-positive bacteria. Injection of lipoteichoic acid (LTA), a key component of Gram-positive bacteria cell wall, into the leech body wall induced a massive migration of granulocytes and macrophages expressing TLR2 (the key receptor involved in the response to Gram-positive bacteria) toward the challenged/inoculated area. We hypothesized that the endogenous leech RNASET2 protein (HvRNASET2) might be involved in the antimicrobial response, as already described for other vertebrate ribonucleases, such as RNase3 and RNase7. In support of our hypothesis, HvRNASET2 was mainly localized in the granules of granulocytes, and its release in the extracellular matrix triggered the recruitment of macrophages toward the area stimulated with LTA. The activity of HvRNASET2 was also evaluated on Staphylococcus aureus living cells by means of light, transmission, and scanning electron microscopy analysis. HvRNASET2 injection triggered the formation of S. aureus clumps following a direct interaction with the bacterial cell wall, as demonstrated by immunogold assay. Taken together, our data support the notion that, during the early phase of leech immune response, granulocyte-released HvRNASET2 triggers bacterial clumps formation and, at the same time, actively recruits phagocytic macrophages in order to elicit a rapid and effective eradication of the infecting microorganisms from inoculated area. SN - 1664-3224 UR - https://www.unboundmedicine.com/medline/citation/32210967/Antimicrobial_Role_of_RNASET2_Protein_During_Innate_Immune_Response_in_the_Medicinal_Leech_Hirudo_verbana L2 - https://doi.org/10.3389/fimmu.2020.00370 DB - PRIME DP - Unbound Medicine ER -
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