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Identification of Arylphorin interacting with the insecticidal protein PirAB from Xenorhabdus nematophila by yeast two-hybrid system.
World J Microbiol Biotechnol. 2020 Mar 25; 36(4):56.WJ

Abstract

PirAB toxin was initially found in the Photorhabdus luminescens TT01 strain and is a demonstrated binary toxin with high insecticidal activity. In this paper, we co-expressed the pirAB gene of Xenorhabdus nematophila HB310 in a prokaryotic expression system, and we found that the PirAB protein showed high hemocoel insecticidal activity against Galleria mellonella, Helicoverpa armigera and Spodoptera exigua. LD50 values were 1.562, 2.003 and 2.17 μg/larvae for G. mellonella, H. armigera, and S. exigua, respectively (p > 0.05). Additionally, PirAB-interaction proteins were identified from G. mellonella by 6 × His Protein Pulldown combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Of which, arylphorin of G. mellonella showed the highest matching rate. A protein domain conservative structure analysis indicated that arylphorin has three domains including Hemocyanin-N, Hemocyanin-M, and Hemocyanin-C. Among these protein domains, Hemocyanin-C has immune and recognition functions. Further, Hemocyanin-C domain of arylphorin was identified to interact with PirA but not PirB by Yeast two-hybrid system. These findings reveal, for the first time, new host protein interacting with PirAB. The identification of interaction protein may serve as the foundation for further study on the function and insecticidal mechanism of this binary toxin from Xenorhabdus.

Authors+Show Affiliations

Plant Protection College, Hebei Agricultural University, Baoding, 071000, China. ngzyheb@163.com.Plant Protection College, Hebei Agricultural University, Baoding, 071000, China.College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.Plant Protection College, Hebei Agricultural University, Baoding, 071000, China.Plant Protection College, Hebei Agricultural University, Baoding, 071000, China.Texas A&M AgriLife Research and Extension Center, Lubbock, TX, 79403, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32211973

Citation

NanGong, Ziyan, et al. "Identification of Arylphorin Interacting With the Insecticidal Protein PirAB From Xenorhabdus Nematophila By Yeast Two-hybrid System." World Journal of Microbiology & Biotechnology, vol. 36, no. 4, 2020, p. 56.
NanGong Z, Guo X, Yang Q, et al. Identification of Arylphorin interacting with the insecticidal protein PirAB from Xenorhabdus nematophila by yeast two-hybrid system. World J Microbiol Biotechnol. 2020;36(4):56.
NanGong, Z., Guo, X., Yang, Q., Song, P., Wang, Q., & Parajulee, M. N. (2020). Identification of Arylphorin interacting with the insecticidal protein PirAB from Xenorhabdus nematophila by yeast two-hybrid system. World Journal of Microbiology & Biotechnology, 36(4), 56. https://doi.org/10.1007/s11274-020-02833-2
NanGong Z, et al. Identification of Arylphorin Interacting With the Insecticidal Protein PirAB From Xenorhabdus Nematophila By Yeast Two-hybrid System. World J Microbiol Biotechnol. 2020 Mar 25;36(4):56. PubMed PMID: 32211973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Arylphorin interacting with the insecticidal protein PirAB from Xenorhabdus nematophila by yeast two-hybrid system. AU - NanGong,Ziyan, AU - Guo,Xiaoxiao, AU - Yang,Qing, AU - Song,Ping, AU - Wang,Qinying, AU - Parajulee,Megha N, Y1 - 2020/03/25/ PY - 2019/10/30/received PY - 2020/03/18/accepted PY - 2020/3/27/entrez PY - 2020/3/27/pubmed PY - 2020/9/24/medline KW - His pull-down·arylphorin KW - PirAB toxin KW - Xenorhabdus nematophila KW - Yeast two-hybrid SP - 56 EP - 56 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 36 IS - 4 N2 - PirAB toxin was initially found in the Photorhabdus luminescens TT01 strain and is a demonstrated binary toxin with high insecticidal activity. In this paper, we co-expressed the pirAB gene of Xenorhabdus nematophila HB310 in a prokaryotic expression system, and we found that the PirAB protein showed high hemocoel insecticidal activity against Galleria mellonella, Helicoverpa armigera and Spodoptera exigua. LD50 values were 1.562, 2.003 and 2.17 μg/larvae for G. mellonella, H. armigera, and S. exigua, respectively (p > 0.05). Additionally, PirAB-interaction proteins were identified from G. mellonella by 6 × His Protein Pulldown combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Of which, arylphorin of G. mellonella showed the highest matching rate. A protein domain conservative structure analysis indicated that arylphorin has three domains including Hemocyanin-N, Hemocyanin-M, and Hemocyanin-C. Among these protein domains, Hemocyanin-C has immune and recognition functions. Further, Hemocyanin-C domain of arylphorin was identified to interact with PirA but not PirB by Yeast two-hybrid system. These findings reveal, for the first time, new host protein interacting with PirAB. The identification of interaction protein may serve as the foundation for further study on the function and insecticidal mechanism of this binary toxin from Xenorhabdus. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/32211973/Identification_of_Arylphorin_interacting_with_the_insecticidal_protein_PirAB_from_Xenorhabdus_nematophila_by_yeast_two_hybrid_system_ L2 - https://dx.doi.org/10.1007/s11274-020-02833-2 DB - PRIME DP - Unbound Medicine ER -