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Dual inoculation of alfalfa (Medicago sativa L.) with Funnelliformis mosseae and Sinorhizobium medicae can reduce Fusarium wilt.
J Appl Microbiol. 2020 Sep; 129(3):665-679.JA

Abstract

AIMS

This study was designed to evaluate the biocontrol of the arbuscular mycorrhizal fungus (AMF) Funnelliformis mosseae and the rhizobium Sinorhizobium medicae on alfalfa (Medicago sativa) wilt caused by Fusarium oxysporum, a severe soil-borne fungal pathogen.

METHODS AND RESULTS

The effects of co-inoculation of F. mosseae and S. medicae on alfalfa growth, nitrogen, phosphorus uptake and wilt caused by F. oxysporum were tested. Plant defence-related chemicals were measured to reveal the biochemical mechanism by which alfalfa responds to pathogen infection and how it is regulated by AMF and rhizobium. Pathogen infection caused typical yellowing of alfalfa leaflets and significantly reduced plant AMF colonization. AMF or rhizobium alone and the co-inoculation reduced the plant disease index by 83·2, 48·4 and 81·8% respectively. Inoculation with AMF or rhizobium alone increased the dry weight of alfalfa by more than 13 and 3 times respectively; it also increased plant chlorophyll content by 65·6 and 16·6% respectively. Co-inoculation of AMF and rhizobium induced the plant to accumulate more disease-related antioxidant enzymes, plant hydrolase and plant hormones, such as superoxide dismutase, β-1,3-glucanase, chitinase, and phenylalanine ammonialyase, abscisic acid, ethylene and H2 O2 , under pathogen stress.

CONCLUSIONS

Co-inoculation with F. mosseae and S. medicae offered complementarily improved alfalfa nutrient uptake and growth, which increased plant health. The co-inoculation of AMF and rhizobium regulated plant physiological and biochemical processes and induced plants to produce defence-related compounds, thus decreasing the severity of disease. The simultaneous application of F. mosseae and S. medicae is a potential biocontrol strategy to increase the systemic defence responses of alfalfa to Fusarium wilt.

SIGNIFICANCE AND IMPACT OF THE STUDY

This research showed that complex plant-pathogen interactions are affected by rhizobium and AMF, providing insight into plant-microbiome interactions in the rhizosphere as well as the application of the microbiome in agriculture production.

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Authors+Show Affiliations

Wang X
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China. MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, P. R. China.
Ding T
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China.
Li Y
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China.
Guo Y
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China.
Li Y
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China.
Duan T
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China.

MeSH

FusariumMedicago sativaMycorrhizaeNutrientsPest Control, BiologicalPlant DiseasesRhizosphereSinorhizobiumSoil Microbiology

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32215998

Citation

Wang, X, et al. "Dual Inoculation of Alfalfa (Medicago Sativa L.) With Funnelliformis Mosseae and Sinorhizobium Medicae Can Reduce Fusarium Wilt." Journal of Applied Microbiology, vol. 129, no. 3, 2020, pp. 665-679.
Wang X, Ding T, Li Y, et al. Dual inoculation of alfalfa (Medicago sativa L.) with Funnelliformis mosseae and Sinorhizobium medicae can reduce Fusarium wilt. J Appl Microbiol. 2020;129(3):665-679.
Wang, X., Ding, T., Li, Y., Guo, Y., Li, Y., & Duan, T. (2020). Dual inoculation of alfalfa (Medicago sativa L.) with Funnelliformis mosseae and Sinorhizobium medicae can reduce Fusarium wilt. Journal of Applied Microbiology, 129(3), 665-679. https://doi.org/10.1111/jam.14645
Wang X, et al. Dual Inoculation of Alfalfa (Medicago Sativa L.) With Funnelliformis Mosseae and Sinorhizobium Medicae Can Reduce Fusarium Wilt. J Appl Microbiol. 2020;129(3):665-679. PubMed PMID: 32215998.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual inoculation of alfalfa (Medicago sativa L.) with Funnelliformis mosseae and Sinorhizobium medicae can reduce Fusarium wilt. AU - Wang,X, AU - Ding,T, AU - Li,Y, AU - Guo,Y, AU - Li,Y, AU - Duan,T, Y1 - 2020/04/13/ PY - 2019/10/20/received PY - 2020/03/02/revised PY - 2020/03/18/accepted PY - 2020/3/28/pubmed PY - 2020/10/21/medline PY - 2020/3/28/entrez KW - biocontrol KW - enzymes KW - fungi KW - plant disease KW - rhizosphere SP - 665 EP - 679 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 129 IS - 3 N2 - AIMS: This study was designed to evaluate the biocontrol of the arbuscular mycorrhizal fungus (AMF) Funnelliformis mosseae and the rhizobium Sinorhizobium medicae on alfalfa (Medicago sativa) wilt caused by Fusarium oxysporum, a severe soil-borne fungal pathogen. METHODS AND RESULTS: The effects of co-inoculation of F. mosseae and S. medicae on alfalfa growth, nitrogen, phosphorus uptake and wilt caused by F. oxysporum were tested. Plant defence-related chemicals were measured to reveal the biochemical mechanism by which alfalfa responds to pathogen infection and how it is regulated by AMF and rhizobium. Pathogen infection caused typical yellowing of alfalfa leaflets and significantly reduced plant AMF colonization. AMF or rhizobium alone and the co-inoculation reduced the plant disease index by 83·2, 48·4 and 81·8% respectively. Inoculation with AMF or rhizobium alone increased the dry weight of alfalfa by more than 13 and 3 times respectively; it also increased plant chlorophyll content by 65·6 and 16·6% respectively. Co-inoculation of AMF and rhizobium induced the plant to accumulate more disease-related antioxidant enzymes, plant hydrolase and plant hormones, such as superoxide dismutase, β-1,3-glucanase, chitinase, and phenylalanine ammonialyase, abscisic acid, ethylene and H2 O2 , under pathogen stress. CONCLUSIONS: Co-inoculation with F. mosseae and S. medicae offered complementarily improved alfalfa nutrient uptake and growth, which increased plant health. The co-inoculation of AMF and rhizobium regulated plant physiological and biochemical processes and induced plants to produce defence-related compounds, thus decreasing the severity of disease. The simultaneous application of F. mosseae and S. medicae is a potential biocontrol strategy to increase the systemic defence responses of alfalfa to Fusarium wilt. SIGNIFICANCE AND IMPACT OF THE STUDY: This research showed that complex plant-pathogen interactions are affected by rhizobium and AMF, providing insight into plant-microbiome interactions in the rhizosphere as well as the application of the microbiome in agriculture production. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/32215998/Dual_inoculation_of_alfalfa__Medicago_sativa_L___with_Funnelliformis_mosseae_and_Sinorhizobium_medicae_can_reduce_Fusarium_wilt_ DB - PRIME DP - Unbound Medicine ER -