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Expanding a Portfolio of (FO-) SPR Surface Chemistries with the Co(III)-NTA Oriented Immobilization of His6-Tagged Bioreceptors for Applications in Complex Matrices.
ACS Sens. 2020 04 24; 5(4):960-969.AS

Abstract

Cobalt-nitrilotriacetic acid (Co(III)-NTA) chemistry is a recognized approach for oriented patterning of His6-tagged bioreceptors. We have applied the matching strategy for the first time on a surface plasmon resonance (SPR) platform, namely, the commercialized fiber optic (FO)-SPR. To accomplish this, His6-tagged bioreceptor (scFv-33H1F7) and its target PAI-1 were used as a model system, after scrutinizing the specificity of their interaction. When benchmarked to traditional carboxyl-based self-assembled monolayers (SAM), NTA allowed (1) more efficient FO-SPR surface coverage with bioreceptors compared with the former and (2) realization of thus far difficult-to-attain label-free bioassays on the FO-SPR platform in both buffer and 20-fold diluted human plasma. Moreover, Co(III)-NTA surface proved to be compatible with traditional gold nanoparticle-mediated signal amplification in the buffer as well as in 10-fold diluted human plasma, thus expanding the dynamic detection range to low ng/mL. Both types of bioassays revealed that scFv-33H1F7 immobilized on the FO-SPR surface using different concentrations (20, 10, or 5 μg/mL) had no impact on the bioassay sensitivity, accuracy, or reproducibility despite the lowest concentration effectively resulting in close to 20% fewer bioreceptors. Collectively, these results highlight the importance of Co(III)-NTA promoting the oriented patterning of bioreceptors on the FO-SPR sensor surface for securing robust and sensitive bioassays in complex matrices, both in label-free and labeled formats.

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Authors+Show Affiliations

Qu JH
Department of Biosystems, Biosensors Group, KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.
Horta S
Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak, 8500 Kortrijk, Belgium.
Delport F
FOx Biosystems, Bioville, Agoralaan Abis, 3590 Diepenbeek, Belgium.
Sillen M
Department of Pharmaceutical and Pharmacological Sciences, Laboratory for Therapeutic and Diagnostic Antibodies, KU Leuven, O&N II Herestraat 49, 3000 Leuven, Belgium.
Geukens N
PharmAbs, KU Leuven, Herestraat 49, Box 820, B 3000 Leuven, Belgium.
Sun DW
School of Food Science and Engineering, South China University of Technology, 510641 Guangzhou, China.
Vanhoorelbeke K
Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak, 8500 Kortrijk, Belgium.
Declerck P
Department of Pharmaceutical and Pharmacological Sciences, Laboratory for Therapeutic and Diagnostic Antibodies, KU Leuven, O&N II Herestraat 49, 3000 Leuven, Belgium.
Lammertyn J
Department of Biosystems, Biosensors Group, KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.
Spasic D
Department of Biosystems, Biosensors Group, KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.

MeSH

Biosensing TechniquesCobaltHumansMetal NanoparticlesSurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32216277

Citation

Qu, Jia-Huan, et al. "Expanding a Portfolio of (FO-) SPR Surface Chemistries With the Co(III)-NTA Oriented Immobilization of His6-Tagged Bioreceptors for Applications in Complex Matrices." ACS Sensors, vol. 5, no. 4, 2020, pp. 960-969.
Qu JH, Horta S, Delport F, et al. Expanding a Portfolio of (FO-) SPR Surface Chemistries with the Co(III)-NTA Oriented Immobilization of His6-Tagged Bioreceptors for Applications in Complex Matrices. ACS Sens. 2020;5(4):960-969.
Qu, J. H., Horta, S., Delport, F., Sillen, M., Geukens, N., Sun, D. W., Vanhoorelbeke, K., Declerck, P., Lammertyn, J., & Spasic, D. (2020). Expanding a Portfolio of (FO-) SPR Surface Chemistries with the Co(III)-NTA Oriented Immobilization of His6-Tagged Bioreceptors for Applications in Complex Matrices. ACS Sensors, 5(4), 960-969. https://doi.org/10.1021/acssensors.9b02227
Qu JH, et al. Expanding a Portfolio of (FO-) SPR Surface Chemistries With the Co(III)-NTA Oriented Immobilization of His6-Tagged Bioreceptors for Applications in Complex Matrices. ACS Sens. 2020 04 24;5(4):960-969. PubMed PMID: 32216277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expanding a Portfolio of (FO-) SPR Surface Chemistries with the Co(III)-NTA Oriented Immobilization of His6-Tagged Bioreceptors for Applications in Complex Matrices. AU - Qu,Jia-Huan, AU - Horta,Sara, AU - Delport,Filip, AU - Sillen,Machteld, AU - Geukens,Nick, AU - Sun,Da-Wen, AU - Vanhoorelbeke,Karen, AU - Declerck,Paul, AU - Lammertyn,Jeroen, AU - Spasic,Dragana, Y1 - 2020/04/08/ PY - 2020/3/29/pubmed PY - 2021/1/12/medline PY - 2020/3/29/entrez KW - bioreceptor orientation KW - fiber optic surface plasmon resonance (FO-SPR) KW - label-free bioassay KW - nitrilotriacetic acid (NTA) KW - plasminogen activator inhibitor-1 (PAI-1) KW - sandwich bioassay SP - 960 EP - 969 JF - ACS sensors JO - ACS Sens VL - 5 IS - 4 N2 - Cobalt-nitrilotriacetic acid (Co(III)-NTA) chemistry is a recognized approach for oriented patterning of His6-tagged bioreceptors. We have applied the matching strategy for the first time on a surface plasmon resonance (SPR) platform, namely, the commercialized fiber optic (FO)-SPR. To accomplish this, His6-tagged bioreceptor (scFv-33H1F7) and its target PAI-1 were used as a model system, after scrutinizing the specificity of their interaction. When benchmarked to traditional carboxyl-based self-assembled monolayers (SAM), NTA allowed (1) more efficient FO-SPR surface coverage with bioreceptors compared with the former and (2) realization of thus far difficult-to-attain label-free bioassays on the FO-SPR platform in both buffer and 20-fold diluted human plasma. Moreover, Co(III)-NTA surface proved to be compatible with traditional gold nanoparticle-mediated signal amplification in the buffer as well as in 10-fold diluted human plasma, thus expanding the dynamic detection range to low ng/mL. Both types of bioassays revealed that scFv-33H1F7 immobilized on the FO-SPR surface using different concentrations (20, 10, or 5 μg/mL) had no impact on the bioassay sensitivity, accuracy, or reproducibility despite the lowest concentration effectively resulting in close to 20% fewer bioreceptors. Collectively, these results highlight the importance of Co(III)-NTA promoting the oriented patterning of bioreceptors on the FO-SPR sensor surface for securing robust and sensitive bioassays in complex matrices, both in label-free and labeled formats. SN - 2379-3694 UR - https://www.unboundmedicine.com/medline/citation/32216277/Expanding_a_Portfolio_of__FO___SPR_Surface_Chemistries_with_the_Co_III__NTA_Oriented_Immobilization_of_His6_Tagged_Bioreceptors_for_Applications_in_Complex_Matrices_ DB - PRIME DP - Unbound Medicine ER -