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Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19.
J Med Virol. 2020 07; 92(7):903-908.JM

Abstract

In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real-time reverse-transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 in the 610 hospitalized patients clinically diagnosed with COVID-19 during the 2019 outbreak. We also found that the RT-PCR results from several tests at different points were variable from the same patients during the course of diagnosis and treatment of these patients. Our results indicate that in addition to the emphasis on RT-PCR testing, clinical indicators such as computed tomography images should also be used not only for diagnosis and treatment but also for isolation, recovery/discharge, and transferring for hospitalized patients clinically diagnosed with COVID-19 during the current epidemic. These results suggested the urgent needs for the standard of procedures of sampling from different anatomic sites, sample transportation, optimization of RT-PCR, serology diagnosis/screening for SARS-CoV-2 infection, and distinct diagnosis from other respiratory diseases such as fluenza infections as well.

Authors+Show Affiliations

Department of Intensive Care Unit, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.Department of Operation and Management, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.Natural Language Processing Group, iFLYTEK Research South China, Guangzhou, China.Department of Intensive Care Unit, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.Department of Clinical Medicine, Zhongshan School of Medicine, Guangzhou, China.Department of Respiratory, Hankou Hospital of Wuhan, Wuhan, China.Department of Intensive Care Unit, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32219885

Citation

Li, Yafang, et al. "Stability Issues of RT-PCR Testing of SARS-CoV-2 for Hospitalized Patients Clinically Diagnosed With COVID-19." Journal of Medical Virology, vol. 92, no. 7, 2020, pp. 903-908.
Li Y, Yao L, Li J, et al. Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19. J Med Virol. 2020;92(7):903-908.
Li, Y., Yao, L., Li, J., Chen, L., Song, Y., Cai, Z., & Yang, C. (2020). Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19. Journal of Medical Virology, 92(7), 903-908. https://doi.org/10.1002/jmv.25786
Li Y, et al. Stability Issues of RT-PCR Testing of SARS-CoV-2 for Hospitalized Patients Clinically Diagnosed With COVID-19. J Med Virol. 2020;92(7):903-908. PubMed PMID: 32219885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19. AU - Li,Yafang, AU - Yao,Lin, AU - Li,Jiawei, AU - Chen,Lei, AU - Song,Yiyan, AU - Cai,Zhifang, AU - Yang,Chunhua, Y1 - 2020/04/05/ PY - 2020/03/10/received PY - 2020/03/21/revised PY - 2020/03/22/accepted PY - 2020/3/29/pubmed PY - 2020/6/25/medline PY - 2020/3/29/entrez KW - COVID-19 KW - RT-PCR SP - 903 EP - 908 JF - Journal of medical virology JO - J Med Virol VL - 92 IS - 7 N2 - In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real-time reverse-transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 in the 610 hospitalized patients clinically diagnosed with COVID-19 during the 2019 outbreak. We also found that the RT-PCR results from several tests at different points were variable from the same patients during the course of diagnosis and treatment of these patients. Our results indicate that in addition to the emphasis on RT-PCR testing, clinical indicators such as computed tomography images should also be used not only for diagnosis and treatment but also for isolation, recovery/discharge, and transferring for hospitalized patients clinically diagnosed with COVID-19 during the current epidemic. These results suggested the urgent needs for the standard of procedures of sampling from different anatomic sites, sample transportation, optimization of RT-PCR, serology diagnosis/screening for SARS-CoV-2 infection, and distinct diagnosis from other respiratory diseases such as fluenza infections as well. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/32219885/Stability_issues_of_RT_PCR_testing_of_SARS_CoV_2_for_hospitalized_patients_clinically_diagnosed_with_COVID_19_ L2 - https://doi.org/10.1002/jmv.25786 DB - PRIME DP - Unbound Medicine ER -