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Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns.
J Prosthet Dent. 2020 Jul; 124(1):122.e1-122.e9.JP

Abstract

STATEMENT OF PROBLEM

If the components in the acrylic resins used to fabricate interim crows are cytotoxic, they can interfere with the integrity of the adjacent periodontal tissue and the dentin-pulp complex.

PURPOSE

The purpose of this in vitro study was to assess the cytotoxicity of resin-based materials used to prepare interim crowns.

MATERIAL AND METHODS

The following materials were used in this study: CAR, conventional acrylic resin powder and liquid; BR, bis-acrylic resin; and PAR, pressed acrylic resin of the CAD-CAM type. Glass disks were used as the control (Co). Oral epithelial cells (NOK) were seeded on glass disks and standardized disks prepared with the resins under study. After incubation for 24 hours, the cells were analyzed for viability (Alamar Blue and Live or Dead), adhesion, and morphology (SEM and fluorescence), as well as epidermal growth factor synthesis (EGF-ELISA). The surface roughness (Ra) of test specimens was evaluated under a confocal microscope. The data were submitted to ANOVA and the Tukey HSD statistical tests (α=.05).

RESULTS

The highest Ra value was observed in BR in comparison with CAR, PAR, and Co (P<.05). The highest viability, adhesion, and EGF synthesis values were determined for the cells in contact with PAR (P<.001).

CONCLUSIONS

The computer-aided design and computer-aided manufacturing (CAD-CAM)-type resin favored adhesion, metabolism, and epithelial cell proliferation, and it was therefore considered cytocompatible.

Authors+Show Affiliations

Masters student, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.Doctoral student, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.Professor, Department of Dentistry, The University of Ribeirão Preto (UNAERP), Ribeirão Preto, Brazil.Professor, Department of Orthodontics and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.Professor, Department of Physiology and Pathology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil. Electronic address: casouzac@foar.unesp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32220399

Citation

Souza, Isabela R., et al. "Cytotoxicity of Acrylic Resin-based Materials Used to Fabricate Interim Crowns." The Journal of Prosthetic Dentistry, vol. 124, no. 1, 2020, pp. 122.e1-122.e9.
Souza IR, Pansani TN, Basso FG, et al. Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns. J Prosthet Dent. 2020;124(1):122.e1-122.e9.
Souza, I. R., Pansani, T. N., Basso, F. G., Hebling, J., & de Souza Costa, C. A. (2020). Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns. The Journal of Prosthetic Dentistry, 124(1), e1-e9. https://doi.org/10.1016/j.prosdent.2020.01.030
Souza IR, et al. Cytotoxicity of Acrylic Resin-based Materials Used to Fabricate Interim Crowns. J Prosthet Dent. 2020;124(1):122.e1-122.e9. PubMed PMID: 32220399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns. AU - Souza,Isabela R, AU - Pansani,Taisa N, AU - Basso,Fernanda G, AU - Hebling,Josimeri, AU - de Souza Costa,Carlos A, Y1 - 2020/03/25/ PY - 2019/09/13/received PY - 2020/01/15/revised PY - 2020/01/15/accepted PY - 2020/3/30/pubmed PY - 2020/7/8/medline PY - 2020/3/30/entrez SP - 122.e1 EP - 122.e9 JF - The Journal of prosthetic dentistry JO - J Prosthet Dent VL - 124 IS - 1 N2 - STATEMENT OF PROBLEM: If the components in the acrylic resins used to fabricate interim crows are cytotoxic, they can interfere with the integrity of the adjacent periodontal tissue and the dentin-pulp complex. PURPOSE: The purpose of this in vitro study was to assess the cytotoxicity of resin-based materials used to prepare interim crowns. MATERIAL AND METHODS: The following materials were used in this study: CAR, conventional acrylic resin powder and liquid; BR, bis-acrylic resin; and PAR, pressed acrylic resin of the CAD-CAM type. Glass disks were used as the control (Co). Oral epithelial cells (NOK) were seeded on glass disks and standardized disks prepared with the resins under study. After incubation for 24 hours, the cells were analyzed for viability (Alamar Blue and Live or Dead), adhesion, and morphology (SEM and fluorescence), as well as epidermal growth factor synthesis (EGF-ELISA). The surface roughness (Ra) of test specimens was evaluated under a confocal microscope. The data were submitted to ANOVA and the Tukey HSD statistical tests (α=.05). RESULTS: The highest Ra value was observed in BR in comparison with CAR, PAR, and Co (P<.05). The highest viability, adhesion, and EGF synthesis values were determined for the cells in contact with PAR (P<.001). CONCLUSIONS: The computer-aided design and computer-aided manufacturing (CAD-CAM)-type resin favored adhesion, metabolism, and epithelial cell proliferation, and it was therefore considered cytocompatible. SN - 1097-6841 UR - https://www.unboundmedicine.com/medline/citation/32220399/Cytotoxicity_of_acrylic_resin-based_materials_used_to_fabricate_interim_crowns L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-3913(20)30100-1 DB - PRIME DP - Unbound Medicine ER -