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Super-kdr mutation M918L and multiple cytochrome P450s associated with the resistance of Rhopalosiphum padi to pyrethroid.
Pest Manag Sci. 2020 Aug; 76(8):2809-2817.PM

Abstract

BACKGROUND

Rhopalosiphum padi is an important pest affecting cereal crops worldwide. Pyrethroid, including lambda-cyhalothrin, has been widely used to control R. padi in the field. This work investigated the resistance levels of R. padi field populations to lambda-cyhalothrin, and analysed biochemical and molecular mechanisms of aphid resistance to the insecticide pyrethroid.

RESULTS

A lambda-cyhalothrin-resistant field population (JY) was sampled, and a super-kdr mutation, M918L, in the voltage-gated sodium channel (VGSC) was identified in the population. The lambda-cyhalothrin-resistant strain (LC-R) was subsequently established by selecting the field population with lambda-cyhalothrin. All individuals of the R. padi LC-R strain showed the M918L heterozygous mutation in the VGSC IIS4-IIS6 region. Cross-resistance profiles of the LC-R strain to nine insecticides were detected. Both synergistic and enzyme activity studies indicated that cytochrome P450 monooxygenase played an important role in this resistance. Further gene expression analysis showed that seven P450 genes were significantly upregulated in the LC-R strain compared with the susceptible strain.

CONCLUSION

Field-evolved resistance to pyrethroid insecticides has been found in R. padi. The M918L (super-kdr) mutation in the VGSC was documented for the first time in field samples obtained from an important wheat-growing area. The super-kdr mutation, as well as metabolic resistance mediated by P450 genes, was determined to contribute to the lambda-cyhalothrin resistance in R. padi. © 2020 Society of Chemical Industry.

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Authors+Show Affiliations

Wang K
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China. Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture and Rural Affairs, Nanjing, China.
Bai J
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
Zhao J
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
Su S
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
Liu L
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.
Han Z
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture and Rural Affairs, Nanjing, China.
Chen M
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, China.

MeSH

AnimalsAphidsCytochrome P-450 Enzyme SystemInsecticide ResistanceMutationPyrethrins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32222020

Citation

Wang, Kang, et al. "Super-kdr Mutation M918L and Multiple Cytochrome P450s Associated With the Resistance of Rhopalosiphum Padi to Pyrethroid." Pest Management Science, vol. 76, no. 8, 2020, pp. 2809-2817.
Wang K, Bai J, Zhao J, et al. Super-kdr mutation M918L and multiple cytochrome P450s associated with the resistance of Rhopalosiphum padi to pyrethroid. Pest Manag Sci. 2020;76(8):2809-2817.
Wang, K., Bai, J., Zhao, J., Su, S., Liu, L., Han, Z., & Chen, M. (2020). Super-kdr mutation M918L and multiple cytochrome P450s associated with the resistance of Rhopalosiphum padi to pyrethroid. Pest Management Science, 76(8), 2809-2817. https://doi.org/10.1002/ps.5829
Wang K, et al. Super-kdr Mutation M918L and Multiple Cytochrome P450s Associated With the Resistance of Rhopalosiphum Padi to Pyrethroid. Pest Manag Sci. 2020;76(8):2809-2817. PubMed PMID: 32222020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Super-kdr mutation M918L and multiple cytochrome P450s associated with the resistance of Rhopalosiphum padi to pyrethroid. AU - Wang,Kang, AU - Bai,Jiaoyang, AU - Zhao,Junning, AU - Su,Sha, AU - Liu,Lang, AU - Han,Zhaojun, AU - Chen,Maohua, Y1 - 2020/04/08/ PY - 2019/12/19/received PY - 2020/02/27/revised PY - 2020/03/28/accepted PY - 2020/3/30/pubmed PY - 2020/9/9/medline PY - 2020/3/30/entrez KW - Rhopalosiphum padi KW - M918L KW - cytochrome P450 KW - lambda-cyhalothrin KW - pyrethroid resistance KW - voltage-gated sodium channel SP - 2809 EP - 2817 JF - Pest management science JO - Pest Manag Sci VL - 76 IS - 8 N2 - BACKGROUND: Rhopalosiphum padi is an important pest affecting cereal crops worldwide. Pyrethroid, including lambda-cyhalothrin, has been widely used to control R. padi in the field. This work investigated the resistance levels of R. padi field populations to lambda-cyhalothrin, and analysed biochemical and molecular mechanisms of aphid resistance to the insecticide pyrethroid. RESULTS: A lambda-cyhalothrin-resistant field population (JY) was sampled, and a super-kdr mutation, M918L, in the voltage-gated sodium channel (VGSC) was identified in the population. The lambda-cyhalothrin-resistant strain (LC-R) was subsequently established by selecting the field population with lambda-cyhalothrin. All individuals of the R. padi LC-R strain showed the M918L heterozygous mutation in the VGSC IIS4-IIS6 region. Cross-resistance profiles of the LC-R strain to nine insecticides were detected. Both synergistic and enzyme activity studies indicated that cytochrome P450 monooxygenase played an important role in this resistance. Further gene expression analysis showed that seven P450 genes were significantly upregulated in the LC-R strain compared with the susceptible strain. CONCLUSION: Field-evolved resistance to pyrethroid insecticides has been found in R. padi. The M918L (super-kdr) mutation in the VGSC was documented for the first time in field samples obtained from an important wheat-growing area. The super-kdr mutation, as well as metabolic resistance mediated by P450 genes, was determined to contribute to the lambda-cyhalothrin resistance in R. padi. © 2020 Society of Chemical Industry. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/32222020/Super_kdr_mutation_M918L_and_multiple_cytochrome_P450s_associated_with_the_resistance_of_Rhopalosiphum_padi_to_pyrethroid_ DB - PRIME DP - Unbound Medicine ER -