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Pseudomonas PS01 Isolated from Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth.
Microorganisms. 2020 Mar 26; 8(4)M

Abstract

The objectives of this study were to evaluate the plant growth promoting effects on Arabidopsis by Pseudomonas sp. strains associated with rhizosphere of crop plants grown in Mekong Delta, Vietnam. Out of all the screened isolates, Pseudomonas PS01 isolated from maize rhizosphere showed the most prominent plant growth promoting effects on Arabidopsis and maize (Zea mays). We also found that PS01 altered root system architecture (RSA). The full genome of PS01 was resolved using high-throughput sequencing. Phylogenetic analysis identified PS01 as a member of the Pseudomonas putida subclade, which is closely related to Pseudomonas taiwanensis.. PS01 genome size is 5.3 Mb, assembled in 71 scaffolds comprising of 4820 putative coding sequence. PS01 encodes genes for the indole-3-acetic acid (IAA), acetoin and 2,3-butanediol biosynthesis pathways. PS01 promoted the growth of Arabidopsis and altered the root system architecture by inhibiting primary root elongation and promoting lateral root and root hair formation. By employing gene expression analysis, genetic screening and pharmacological approaches, we suggested that the plant-growth promoting effects of PS01 and the alteration of RSA might be independent of bacterial auxin and could be caused by a combination of different diffusible compounds and volatile organic compounds (VOCs). Taken together, our results suggest that PS01 is a potential candidate to be used as bio-fertilizer agent for enhancing plant growth.

Authors+Show Affiliations

Faculty of Biology and Biotechnology, University of Science-Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. Vietnam National University, Ho Chi Minh City 700000, Vietnam.Faculty of Biology and Biotechnology, University of Science-Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. Vietnam National University, Ho Chi Minh City 700000, Vietnam.Faculty of Biology and Biotechnology, University of Science-Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. Vietnam National University, Ho Chi Minh City 700000, Vietnam.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32224990

Citation

Chu, Thanh Nguyen, et al. "Pseudomonas PS01 Isolated From Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth." Microorganisms, vol. 8, no. 4, 2020.
Chu TN, Bui LV, Hoang MTT. Pseudomonas PS01 Isolated from Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth. Microorganisms. 2020;8(4).
Chu, T. N., Bui, L. V., & Hoang, M. T. T. (2020). Pseudomonas PS01 Isolated from Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth. Microorganisms, 8(4). https://doi.org/10.3390/microorganisms8040471
Chu TN, Bui LV, Hoang MTT. Pseudomonas PS01 Isolated From Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth. Microorganisms. 2020 Mar 26;8(4) PubMed PMID: 32224990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pseudomonas PS01 Isolated from Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth. AU - Chu,Thanh Nguyen, AU - Bui,Le Van, AU - Hoang,Minh Thi Thanh, Y1 - 2020/03/26/ PY - 2020/01/31/received PY - 2020/03/14/revised PY - 2020/03/23/accepted PY - 2020/4/1/entrez PY - 2020/4/1/pubmed PY - 2020/4/1/medline KW - Indole-3-acetic acid (IAA) KW - Plant growth-promoting rhizobacteria (PGPR) KW - Pseudomonas KW - Root system architecture (RSA) KW - volatile organic compounds (VOCs) KW - whole-genome sequencing JF - Microorganisms JO - Microorganisms VL - 8 IS - 4 N2 - The objectives of this study were to evaluate the plant growth promoting effects on Arabidopsis by Pseudomonas sp. strains associated with rhizosphere of crop plants grown in Mekong Delta, Vietnam. Out of all the screened isolates, Pseudomonas PS01 isolated from maize rhizosphere showed the most prominent plant growth promoting effects on Arabidopsis and maize (Zea mays). We also found that PS01 altered root system architecture (RSA). The full genome of PS01 was resolved using high-throughput sequencing. Phylogenetic analysis identified PS01 as a member of the Pseudomonas putida subclade, which is closely related to Pseudomonas taiwanensis.. PS01 genome size is 5.3 Mb, assembled in 71 scaffolds comprising of 4820 putative coding sequence. PS01 encodes genes for the indole-3-acetic acid (IAA), acetoin and 2,3-butanediol biosynthesis pathways. PS01 promoted the growth of Arabidopsis and altered the root system architecture by inhibiting primary root elongation and promoting lateral root and root hair formation. By employing gene expression analysis, genetic screening and pharmacological approaches, we suggested that the plant-growth promoting effects of PS01 and the alteration of RSA might be independent of bacterial auxin and could be caused by a combination of different diffusible compounds and volatile organic compounds (VOCs). Taken together, our results suggest that PS01 is a potential candidate to be used as bio-fertilizer agent for enhancing plant growth. SN - 2076-2607 UR - https://www.unboundmedicine.com/medline/citation/32224990/Pseudomonas_PS01_Isolated_from_Maize_Rhizosphere_Alters_Root_System_Architecture_and_Promotes_Plant_Growth L2 - https://www.mdpi.com/resolver?pii=microorganisms8040471 DB - PRIME DP - Unbound Medicine ER -
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